Current challenges in designer cellulosome engineering.
Biomass
Designer cellulosome
In vitro assembly
Lignocellulose
Protein engineering
Yeast cell surface display
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
May 2023
May 2023
Historique:
received:
27
01
2023
accepted:
03
03
2023
revised:
28
02
2023
medline:
18
4
2023
pubmed:
22
3
2023
entrez:
21
3
2023
Statut:
ppublish
Résumé
Designer cellulosomes (DCs) are engineered multi-enzyme complexes, comprising carbohydrate-active enzymes attached to a common backbone, the scaffoldin, via high-affinity cohesin-dockerin interactions. The use of DCs in the degradation of renewable biomass polymers is a promising approach for biorefineries. Indeed, DCs have shown significant hydrolytic activities due to the enhanced enzyme-substrate proximity and inter-enzyme synergies, but technical hurdles in DC engineering have hindered further progress towards industrial application. The challenge in DC engineering lies in the large diversity of possible building blocks and architectures, resulting in a multivariate and immense design space. Simultaneously, the precise DC composition affects many relevant parameters such as activity, stability, and manufacturability. Since protein engineers face a lack of high-throughput approaches to explore this vast design space, DC engineering may result in an unsatisfying outcome. This review provides a roadmap to guide researchers through the process of DC engineering. Each step, starting from concept to evaluation, is described and provided with its challenges, along with possible solutions, both for DCs that are assembled in vitro or are displayed on the yeast cell surface. KEY POINTS: • Construction of designer cellulosomes is a multi-step process. • Designer cellulosome research deals with multivariate construction challenges. • Boosting designer cellulosome efficiency requires exploring a vast design space.
Identifiants
pubmed: 36941434
doi: 10.1007/s00253-023-12474-8
pii: 10.1007/s00253-023-12474-8
doi:
Substances chimiques
Cellulose
9004-34-6
Cell Cycle Proteins
0
Multienzyme Complexes
0
Bacterial Proteins
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
2755-2770Subventions
Organisme : Fonds Wetenschappelijk Onderzoek
ID : 1SE2623N
Organisme : Fonds Wetenschappelijk Onderzoek
ID : 1S20622N
Organisme : Bijzonder Onderzoeksfonds UGent
ID : BOF17/DOC/086
Organisme : Bijzonder Onderzoeksfonds UGent
ID : BOF16/STA/024
Organisme : Industrieel Onderzoeksfonds UGent
ID : F2020/IOF-STARTT/067
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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