A novel 3D culture model for human primary mammary adipocytes to study their metabolic crosstalk with breast cancer in lean and obese conditions.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
22 03 2023
22 03 2023
Historique:
received:
04
01
2023
accepted:
15
03
2023
entrez:
23
3
2023
pubmed:
24
3
2023
medline:
25
3
2023
Statut:
epublish
Résumé
Obesity is a negative prognosis factor for breast cancer. Yet, the biological mechanisms underlying this effect are still largely unknown. An emerging hypothesis is that the transfer of free fatty acids (FFA) between adipocytes and tumor cells might be altered under obese conditions, contributing to tumor progression. Currently there is a paucity of models to study human mammary adipocytes (M-Ads)-cancer crosstalk. As for other types of isolated white adipocytes, herein, we showed that human M-Ads die within 2-3 days by necrosis when grown in 2D. As an alternative, M-Ads were grown in a fibrin matrix, a 3D model that preserve their distribution, integrity and metabolic function for up to 5 days at physiological glucose concentrations (5 mM). Higher glucose concentrations frequently used in in vitro models promote lipogenesis during M-Ads culture, impairing their lipolytic function. Using transwell inserts, the matrix embedded adipocytes were cocultured with breast cancer cells. FFA transfer between M-Ads and cancer cells was observed, and this event was amplified by obesity. Together these data show that our 3D model is a new tool for studying the effect of M-Ads on tumor cells and beyond with all the components of the tumor microenvironment including the immune cells.
Identifiants
pubmed: 36949082
doi: 10.1038/s41598-023-31673-x
pii: 10.1038/s41598-023-31673-x
pmc: PMC10033714
doi:
Substances chimiques
Fatty Acids, Nonesterified
0
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4707Informations de copyright
© 2023. The Author(s).
Références
J Physiol. 2022 Feb;600(4):869-883
pubmed: 34387376
Am J Physiol Cell Physiol. 2021 May 1;320(5):C822-C841
pubmed: 33439778
JCI Insight. 2017 Feb 23;2(4):e87489
pubmed: 28239646
NPJ Breast Cancer. 2017 May 3;3:19
pubmed: 28649659
Cancer Prev Res (Phila). 2011 Jul;4(7):1021-9
pubmed: 21622727
J Lipid Res. 1999 Nov;40(11):2059-66
pubmed: 10553009
Mol Cell Biol. 2015 May;35(10):1686-99
pubmed: 25733684
EMBO J. 2020 Feb 3;39(3):e102525
pubmed: 31919869
CA Cancer J Clin. 2018 Nov;68(6):394-424
pubmed: 30207593
Cell Rep. 2020 Jan 28;30(4):949-958.e6
pubmed: 31995765
J Gerontol A Biol Sci Med Sci. 1999 Feb;54(2):B71-7
pubmed: 10051853
Cell Rep Med. 2021 Sep 22;2(10):100407
pubmed: 34755127
JCI Insight. 2018 Oct 4;3(19):
pubmed: 30282827
Nat Rev Endocrinol. 2019 Mar;15(3):139-154
pubmed: 30459447
Cancer Res. 2005 Dec 1;65(23):10862-71
pubmed: 16322233
Int J Obes (Lond). 2021 Jan;45(1):195-205
pubmed: 32934318
Ann Oncol. 2014 Oct;25(10):1901-1914
pubmed: 24769692
J Biol Chem. 1964 Feb;239:375-80
pubmed: 14169133
Cancer Lett. 2012 Nov 28;324(2):142-51
pubmed: 22643115
Proteomics. 2010 May;10(9):1886-90
pubmed: 20162561
J Lipid Res. 1990 Aug;31(8):1351-9
pubmed: 2280178
STAR Protoc. 2020 Dec 11;1(3):100197
pubmed: 33377091
Trends Cancer. 2018 May;4(5):374-384
pubmed: 29709261
Cold Spring Harb Perspect Biol. 2011 Dec 01;3(12):
pubmed: 21917992
J Pathol. 2014 Jun;233(2):183-95
pubmed: 24623048
Am J Physiol Cell Physiol. 2017 May 1;312(5):C624-C626
pubmed: 28228375
Am J Physiol Cell Physiol. 2021 Mar 1;320(3):C375-C391
pubmed: 33356944
J Clin Invest. 2019 Oct 1;129(10):3990-4000
pubmed: 31573548
J Biol Chem. 1960 Feb;235:333-6
pubmed: 13796287
Oncotarget. 2017 May 20;8(34):57622-57641
pubmed: 28915700
JAMA Oncol. 2015 Aug;1(5):611-21
pubmed: 26182172
Cell Rep. 2019 Apr 2;27(1):213-225.e5
pubmed: 30943403
J Lipid Res. 1995 Apr;36(4):868-75
pubmed: 7616129
Trends Cancer. 2020 Jul;6(7):593-604
pubmed: 32610069
Annu Rev Nutr. 2007;27:79-101
pubmed: 17313320
Cancer Res. 2011 Apr 1;71(7):2455-65
pubmed: 21459803
Biochem Pharmacol. 1979 Mar 15;28(6):733-6
pubmed: 222292
J Clin Invest. 2012 Nov;122(11):4243-56
pubmed: 23041627
Am J Physiol Cell Physiol. 2012 Jan 15;302(2):C327-59
pubmed: 21900692
Cell Metab. 2018 Jan 9;27(1):68-83
pubmed: 29320711
Diabetes. 2004 May;53(5):1285-92
pubmed: 15111498
Proc Natl Acad Sci U S A. 1980 Jan;77(1):285-9
pubmed: 6928620
Mol Cancer Res. 2019 Mar;17(3):821-835
pubmed: 30606769
Clin Sci (Lond). 1995 Oct;89(4):421-9
pubmed: 7493443
J Lipid Res. 1988 May;29(5):691-7
pubmed: 3411243
J Biol Chem. 2020 Sep 18;295(38):13250-13266
pubmed: 32723868