Proteoliposomes reconstituted with human aquaporin-1 reveal novel single-ion-channel properties.


Journal

Biophysical reports
ISSN: 2667-0747
Titre abrégé: Biophys Rep (N Y)
Pays: United States
ID NLM: 9918266001106676

Informations de publication

Date de publication:
08 Mar 2023
Historique:
received: 04 09 2022
accepted: 11 01 2023
entrez: 23 3 2023
pubmed: 24 3 2023
medline: 24 3 2023
Statut: epublish

Résumé

Human aquaporin 1 (hAQP1) forms homotetrameric channels that facilitate fluxes of water and small solutes across cell membranes. In addition to water channel activity, hAQP1 displays non-selective monovalent cation-channel activity gated by intracellular cyclic GMP. Dual water and ion-channel activity of hAQP1, thought to regulate cell shape and volume, could offer a target for novel therapeutics relevant to controlling cancer cell invasiveness. This study probed properties of hAQP1 ion channels using proteoliposomes, which, unlike conventional cell-based systems such as

Identifiants

pubmed: 36949749
doi: 10.1016/j.bpr.2023.100100
pii: S2667-0747(23)00001-0
pmc: PMC10025285
doi:

Types de publication

Journal Article

Langues

eng

Pagination

100100

Informations de copyright

© 2023 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

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Auteurs

Sam W Henderson (SW)

School of Biomedicine, University of Adelaide, Adelaide, SA 5005, Australia.

Yoshitaka Nakayama (Y)

Victor Chang Cardiac Research Institute, Lowy Packer Building, Darlinghurst, NSW 2010, Australia.
School of Clinical Medicine, UNSW Medicine & Health, St Vincent's Healthcare Clinical Campus, Faculty of Medicine and Health, University of New South Wales, Sydney, NSW Australia.

Murray L Whitelaw (ML)

Institute of Photonics and Advanced Sensing, The School of Biological Sciences, University of Adelaide, Adelaide, SA 5005, Australia.

John B Bruning (JB)

Institute of Photonics and Advanced Sensing, The School of Biological Sciences, University of Adelaide, Adelaide, SA 5005, Australia.

Peter A Anderson (PA)

School of Biological Sciences, Faculty of Science and Engineering, Flinders University, Adelaide, SA 5001, Australia.

Stephen D Tyerman (SD)

ARC Centre of Excellence in Plant Energy Biology, School of Agriculture, Food and Wine & Waite Research Institute, University of Adelaide, Glen Osmond, SA 5064, Australia.

Sunita A Ramesh (SA)

School of Biological Sciences, Faculty of Science and Engineering, Flinders University, Adelaide, SA 5001, Australia.

Boris Martinac (B)

Victor Chang Cardiac Research Institute, Lowy Packer Building, Darlinghurst, NSW 2010, Australia.
School of Clinical Medicine, UNSW Medicine & Health, St Vincent's Healthcare Clinical Campus, Faculty of Medicine and Health, University of New South Wales, Sydney, NSW Australia.

Andrea J Yool (AJ)

School of Biomedicine, University of Adelaide, Adelaide, SA 5005, Australia.

Classifications MeSH