Increased lentivirus titer using ultra expression vectors.

Lentivirus / genetics Transduction, Genetic Genetic Vectors / genetics Base Sequence
Lentivirus Packaging system Transcriptional factors Ultra-expression vectors

Journal

Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535

Informations de publication

Date de publication:
15 05 2023
Historique:
received: 08 02 2023
revised: 13 03 2023
accepted: 14 03 2023
medline: 10 4 2023
pubmed: 24 3 2023
entrez: 23 3 2023
Statut: ppublish

Résumé

Lentivirus is an efficient gene transfer system that is widely used in basic science. We aimed to improve viral titer by applying an ultra-expression vectors to lentiviral packaging. Application of the ultra-expression vectors increased biological titer 4 times for standard preparation. We also evaluated the efficacy of the ultra-expression vectors to relatively longer insert fragments, such as CSII-CMV-CNROE containing 5 genes in multiple cloning sites. Packaging of the ultra-expression vectors showed 3.5 times higher biological titer compared with the original method. Our improved packaging system could be applied to lentivirus to produce higher titers.

Identifiants

DOI: 10.1016/j.ab.2023.115119 PMID: 36958509
pubmed: 36958509
pii: S0003-2697(23)00084-2
doi: 10.1016/j.ab.2023.115119
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

115119

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest All authors do not have any conflict of interest and competing interest to disclose.

Auteurs

So Fujibayashi (S)

Graduate School of Science and Engineering, Iwate University, 4-3-5, Ueda, Morioka, Iwate, 020-8551, Japan.

Tohru Kiyono (T)

Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, 6-5-1, Kashiwanoha, Kashiwa, Chiba, 277-8577, Japan. Electronic address: tkiyono@east.ncc.go.jp.

Yuka Endo (Y)

Graduate School of Science and Engineering, Iwate University, 4-3-5, Ueda, Morioka, Iwate, 020-8551, Japan.

Tetsuya Tani (T)

Laboratory of Animal Reproduction, Department of Agriculture, Kindai University, Nara, Japan.

Haruka Tate (H)

Graduate School of Science and Engineering, Iwate University, 4-3-5, Ueda, Morioka, Iwate, 020-8551, Japan.

Lanlan Bai (L)

Graduate School of Science and Engineering, Iwate University, 4-3-5, Ueda, Morioka, Iwate, 020-8551, Japan.

Eriko Sugano (E)

Graduate School of Science and Engineering, Iwate University, 4-3-5, Ueda, Morioka, Iwate, 020-8551, Japan.

Hiroshi Tomita (H)

Graduate School of Science and Engineering, Iwate University, 4-3-5, Ueda, Morioka, Iwate, 020-8551, Japan.

Tomokazu Fukuda (T)

Graduate School of Science and Engineering, Iwate University, 4-3-5, Ueda, Morioka, Iwate, 020-8551, Japan. Electronic address: tomofukuda009@gmail.com.

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Classifications MeSH

questionsmedicales.fr › Virus › Virus à ARN › Retroviridae › Lentivirus › Increased lentivirus titer using ultra...
questionsmedicales.fr › Phénomènes génétiques › Recombinaison génétique › Transduction génétique › Increased lentivirus titer using ultra...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Vecteurs génétiques › Increased lentivirus titer using ultra...
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence nucléotidique › Increased lentivirus titer using ultra...