Monkeypox virus isolation from longitudinal samples from four patients to infer risk of onwards transmission: an interim analysis.

Animals Chlorocebus aethiops Mpox (monkeypox) / diagnosis Monkeypox virus / genetics Polymerase Chain Reaction
Infectivity Monkeypox Mpox Transmission Viral culture

Journal

The Journal of hospital infection
ISSN: 1532-2939
Titre abrégé: J Hosp Infect
Pays: England
ID NLM: 8007166

Informations de publication

Date de publication:
May 2023
Historique:
received: 03 01 2023
revised: 10 03 2023
accepted: 14 03 2023
medline: 23 5 2023
pubmed: 24 3 2023
entrez: 23 3 2023
Statut: ppublish

Résumé

Monkeypox virus (mpxv) is a DNA virus in the Orthopoxvirus genus which causes Mpox (previously monkeypox). Symptoms include fever, lymphadenopathy and vesicular lesions. There is limited evidence for the duration of mpxv infectivity. This study used cell culture as a proxy for infectivity. Clinical samples from four patients with Mpox were inoculated into African green monkey kidney (Vero E6) cells and monitored for cytopathic effects (CPE). From one patient, infectious mpxv was recovered 25 days after illness onset. Infectious virus was not isolated from samples with an Orthopoxvirus polymerase chain reaction (PCR) Ct value over 31.0, nor from urine.

Identifiants

DOI: 10.1016/j.jhin.2023.03.009 PMID: 36958701 PMC: PMC10029343
pubmed: 36958701
pii: S0195-6701(23)00095-6
doi: 10.1016/j.jhin.2023.03.009
pmc: PMC10029343
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

139-144

Informations de copyright

Crown Copyright © 2023. Published by Elsevier Ltd. All rights reserved.

Références

Emerg Infect Dis. 2020 Apr;26(4):782-785
pubmed: 32023204
Lancet Microbe. 2023 Jan;4(1):e21-e28
pubmed: 36436538
Emerg Infect Dis. 2022 Dec;28(12):2520-2523
pubmed: 36178142
J Clin Med. 2022 Oct 28;11(21):
pubmed: 36362616
J Virol Methods. 2010 Oct;169(1):223-7
pubmed: 20643162
Lancet Infect Dis. 2023 Apr;23(4):445-453
pubmed: 36521505
Lancet Infect Dis. 2022 Aug;22(8):1153-1162
pubmed: 35623380
Environ Microbiol. 2022 Oct;24(10):4561-4569
pubmed: 35837859
Euro Surveill. 2022 Sep;27(35):
pubmed: 36052723

Auteurs

H Callaby (H)

Rare and Imported Pathogens Laboratory, UKHSA, Porton Down, UK; Aberdeen Royal Infirmary, Aberdeen, UK. Electronic address: helen.callaby@nhs.scot.

K Emery (K)

High Containment & Biological Services, Salisbury, UK.

M Killip (M)

High Containment & Biological Services, Salisbury, UK.

T Rampling (T)

Rare and Imported Pathogens Laboratory, UKHSA, Porton Down, UK; The Hospital for Tropical Diseases, University College London Hospital, London, UK; NIHR University College London Hospitals BRC, London, UK.

K S Richards (KS)

High Containment & Biological Services, Salisbury, UK; Faculty of Health and Life Sciences, Oxford Brookes University, Headington Campus, Oxford, UK.

C F Houlihan (CF)

Rare and Imported Pathogens Laboratory, UKHSA, Porton Down, UK; Clinical Virology, University College London Hospital, London, UK; Department of Infection and Immunity, University College London, London, UK.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Monkeypox virus isolation from longitudinal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Cercopithecidae › Cercopithecinae › Cercopithecus › Cercopithecus aethiops › Monkeypox virus isolation from longitudinal...
questionsmedicales.fr › Virus › Virus à ADN › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Virus de la variole simienne › Monkeypox virus isolation from longitudinal...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Monkeypox virus isolation from longitudinal...