Weakly supervised deep learning to predict recurrence in low-grade endometrial cancer from multiplexed immunofluorescence images.


Journal

NPJ digital medicine
ISSN: 2398-6352
Titre abrégé: NPJ Digit Med
Pays: England
ID NLM: 101731738

Informations de publication

Date de publication:
23 Mar 2023
Historique:
received: 05 08 2022
accepted: 10 03 2023
entrez: 24 3 2023
pubmed: 25 3 2023
medline: 25 3 2023
Statut: epublish

Résumé

Predicting recurrence in low-grade, early-stage endometrial cancer (EC) is both challenging and clinically relevant. We present a weakly-supervised deep learning framework, NaroNet, that can learn, without manual expert annotation, the complex tumor-immune interrelations at three levels: local phenotypes, cellular neighborhoods, and tissue areas. It uses multiplexed immunofluorescence for the simultaneous visualization and quantification of CD68 + macrophages, CD8 + T cells, FOXP3 + regulatory T cells, PD-L1/PD-1 protein expression, and tumor cells. We used 489 tumor cores from 250 patients to train a multilevel deep-learning model to predict tumor recurrence. Using a tenfold cross-validation strategy, our model achieved an area under the curve of 0.90 with a 95% confidence interval of 0.83-0.95. Our model predictions resulted in concordance for 96,8% of cases (κ = 0.88). This method could accurately assess the risk of recurrence in EC, outperforming current prognostic factors, including molecular subtyping.

Identifiants

pubmed: 36959234
doi: 10.1038/s41746-023-00795-x
pii: 10.1038/s41746-023-00795-x
pmc: PMC10036616
doi:

Types de publication

Journal Article

Langues

eng

Pagination

48

Subventions

Organisme : Ministry of Economy and Competitiveness | Instituto de Salud Carlos III (Institute of Health Carlos III)
ID : PI17/01723 and PI21/00920

Informations de copyright

© 2023. The Author(s).

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Auteurs

Daniel Jiménez-Sánchez (D)

Program of Solid Tumors and Biomarkers, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain.
Department of Pathology, Clínica Universidad de Navarra, Pamplona, Spain.

Álvaro López-Janeiro (Á)

Department of Pathology, Clínica Universidad de Navarra, Pamplona, Spain.
Department of Pathology, Hospital Universitario La Paz, IdiPAZ, Madrid, Spain.

María Villalba-Esparza (M)

Department of Pathology, Clínica Universidad de Navarra, Pamplona, Spain.
Navarra Institute for Health Research (IdISNA), Pamplona, Spain.

Mikel Ariz (M)

Program of Solid Tumors and Biomarkers, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain.
Navarra Institute for Health Research (IdISNA), Pamplona, Spain.

Ece Kadioglu (E)

Lunaphore Technologies SA, Tolochenaz, Switzerland.

Ivan Masetto (I)

Akoya Biosciences, Marlborough, MA, USA.

Virginie Goubert (V)

Akoya Biosciences, Marlborough, MA, USA.

Maria D Lozano (MD)

Department of Pathology, Clínica Universidad de Navarra, Pamplona, Spain.
Navarra Institute for Health Research (IdISNA), Pamplona, Spain.
Center for Biomedical Research in the Cancer Network (CIBERONC), Madrid, Spain.

Ignacio Melero (I)

Navarra Institute for Health Research (IdISNA), Pamplona, Spain.
Center for Biomedical Research in the Cancer Network (CIBERONC), Madrid, Spain.
Department of Immunology and Immunotherapy, Clínica Universidad de Navarra, Pamplona, Spain.
Program of Immunology and Immunotherapy, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain.

David Hardisson (D)

Department of Pathology, Hospital Universitario La Paz, IdiPAZ, Madrid, Spain.
Center for Biomedical Research in the Cancer Network (CIBERONC), Madrid, Spain.
Molecular Pathology and Therapeutic Targets Group, La Paz University Hospital, IdiPAZ, Madrid, Spain.
Faculty of Medicine, Universidad Autónoma de Madrid, Madrid, Spain.

Carlos Ortiz-de-Solórzano (C)

Program of Solid Tumors and Biomarkers, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain.
Navarra Institute for Health Research (IdISNA), Pamplona, Spain.
Center for Biomedical Research in the Cancer Network (CIBERONC), Madrid, Spain.

Carlos E de Andrea (CE)

Department of Pathology, Clínica Universidad de Navarra, Pamplona, Spain. ceandrea@unav.es.
Navarra Institute for Health Research (IdISNA), Pamplona, Spain. ceandrea@unav.es.
Center for Biomedical Research in the Cancer Network (CIBERONC), Madrid, Spain. ceandrea@unav.es.

Classifications MeSH