Stress Response of European Common Frog (Rana temporaria) Tadpoles to Bti Exposure in an Outdoor Pond Mesocosm.
Amphibians
Bacillus thuringiensis israelensis
GST
Mosquito control
Protein carbonyl
Journal
Bulletin of environmental contamination and toxicology
ISSN: 1432-0800
Titre abrégé: Bull Environ Contam Toxicol
Pays: United States
ID NLM: 0046021
Informations de publication
Date de publication:
24 Mar 2023
24 Mar 2023
Historique:
received:
23
01
2023
accepted:
25
02
2023
entrez:
24
3
2023
pubmed:
25
3
2023
medline:
28
3
2023
Statut:
epublish
Résumé
The biocide Bacillus thuringiensis var. israelensis (Bti) is applied to wetlands to control nuisance by mosquitoes. Amphibians inhabiting these wetlands can be exposed to Bti multiple times, potentially inducing oxidative stress in developing tadpoles. For biochemical stress responses, ambient water temperature plays a key role. Therefore, we exposed tadpoles of the European common frog (Rana temporaria) three times to field-relevant doses of Bti in outdoor floodplain pond mesocosms (FPM) under natural environmental conditions. We sampled tadpoles after each Bti application over the course of a 51-day experiment (April to June 2021) and investigated the activity of the glutathione-S-transferase (GST) and protein carbonyl content as a measure for detoxification activity and oxidative damage. GST activity increased over the course of the experiment likely due to a general increase of water temperature. We did not observe an effect of Bti on either of the investigated biomarkers under natural ambient temperatures. However, Bti-induced effects may be concealed by the generally low water temperatures in our FPMs, particularly at the first application in April, when we expected the highest effect on the most sensitive early stage tadpoles. In light of the global climate change, temperature-related effects of pesticides and biocides on tadpoles should be carefully monitored - in particular since they are known as one of the factors driving the worldwide decline of amphibian populations.
Identifiants
pubmed: 36959482
doi: 10.1007/s00128-023-03708-6
pii: 10.1007/s00128-023-03708-6
pmc: PMC10036417
doi:
Substances chimiques
Disinfectants
0
Glutathione Transferase
EC 2.5.1.18
Water
059QF0KO0R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
70Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : 326210499/GRK2360
Informations de copyright
© 2023. The Author(s).
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