The CB1 cannabinoid receptor regulates autophagy in the tibialis anterior skeletal muscle in mice.
Autophagy
Endocannabinoid receptor
High-fat diet
Skeletal muscle
Journal
Biological research
ISSN: 0717-6287
Titre abrégé: Biol Res
Pays: England
ID NLM: 9308271
Informations de publication
Date de publication:
25 Mar 2023
25 Mar 2023
Historique:
received:
26
08
2022
accepted:
15
03
2023
medline:
28
3
2023
entrez:
25
3
2023
pubmed:
26
3
2023
Statut:
epublish
Résumé
The endocannabinoid system (ECS) regulates energy metabolism, has been implicated in the pathogenesis of metabolic diseases and exerts its actions mainly through the type 1 cannabinoid receptor (CB1). Likewise, autophagy is involved in several cellular processes. It is required for the normal development of muscle mass and metabolism, and its deregulation is associated with diseases. It is known that the CB1 regulates signaling pathways that control autophagy, however, it is currently unknown whether the ECS could regulate autophagy in the skeletal muscle of obese mice. This study aimed to investigate the role of the CB1 in regulating autophagy in skeletal muscle. We found concomitant deregulation in the ECS and autophagy markers in high-fat diet-induced obesity. In obese CB1-KO mice, the autophagy-associated protein LC3 II does not accumulate when mTOR and AMPK phosphorylation levels do not change. Acute inhibition of the CB1 with JD-5037 decreased LC3 II protein accumulation and autophagic flux. Our results suggest that the CB1 regulates autophagy in the tibialis anterior skeletal muscle in both lean and obese mice.
Identifiants
pubmed: 36964619
doi: 10.1186/s40659-023-00426-5
pii: 10.1186/s40659-023-00426-5
pmc: PMC10039507
doi:
Substances chimiques
Cannabinoids
0
Receptor, Cannabinoid, CB1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
14Subventions
Organisme : Agencia Nacional de Investigación y Desarrollo
ID : FONDECYT 1191078
Informations de copyright
© 2023. The Author(s).
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