The challenges of defining the human nasopharyngeal resistome.
antimicrobial resistance
metagenomics
nasopharyngeal
resistome
Journal
Trends in microbiology
ISSN: 1878-4380
Titre abrégé: Trends Microbiol
Pays: England
ID NLM: 9310916
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
received:
06
11
2022
revised:
16
02
2023
accepted:
20
02
2023
medline:
13
7
2023
pubmed:
27
3
2023
entrez:
26
3
2023
Statut:
ppublish
Résumé
The nasopharynx is an important microbial reservoir for the emergence and spread of antibiotic-resistant organisms. The nasopharyngeal resistome is an extensive, adaptable reservoir of antibiotic-resistance genes (ARGs) within this niche. Metagenomic sequencing decodes the genetic material of all organisms within a sample using next-generation technologies, permitting unbiased discovery of novel ARGs and associated mobile genetic elements (MGEs). The challenges of sequencing a low-biomass bacterial sample have limited exploration of the nasopharyngeal resistome. Here, we explore the current understanding of the nasopharyngeal resistome, particularly the role of MGEs in propagating antimicrobial resistance (AMR), explore the advantages and limitations of metagenomic sequencing technologies and bioinformatic pipelines for nasopharyngeal resistome analysis, and highlight the key outstanding questions for future research.
Identifiants
pubmed: 36967247
pii: S0966-842X(23)00056-2
doi: 10.1016/j.tim.2023.02.008
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Review
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
816-831Subventions
Organisme : Department of Health
Pays : United Kingdom
Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests No interests are declared.