A Cold-Active Flavin-Dependent Monooxygenase from


Journal

ACS catalysis
ISSN: 2155-5435
Titre abrégé: ACS Catal
Pays: United States
ID NLM: 101562209

Informations de publication

Date de publication:
17 Mar 2023
Historique:
received: 19 10 2022
revised: 31 01 2023
entrez: 27 3 2023
pubmed: 28 3 2023
medline: 28 3 2023
Statut: epublish

Résumé

Cold-active enzymes maintain a large part of their optimal activity at low temperatures. Therefore, they can be used to avoid side reactions and preserve heat-sensitive compounds. Baeyer-Villiger monooxygenases (BVMO) utilize molecular oxygen as a co-substrate to catalyze reactions widely employed for steroid, agrochemical, antibiotic, and pheromone production. Oxygen has been described as the rate-limiting factor for some BVMO applications, thereby hindering their efficient utilization. Considering that oxygen solubility in water increases by 40% when the temperature is decreased from 30 to 10 °C, we set out to identify and characterize a cold-active BVMO. Using genome mining in the Antarctic organism

Identifiants

pubmed: 36970468
doi: 10.1021/acscatal.2c05160
pmc: PMC10028610
doi:

Types de publication

Journal Article

Langues

eng

Pagination

3549-3562

Informations de copyright

© 2023 The Authors. Published by American Chemical Society.

Déclaration de conflit d'intérêts

The authors declare no competing financial interest.

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Auteurs

Andrea M Chánique (AM)

NAWI Graz, BioTechMed-Graz, Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, Graz 8010, Austria.
Department of Chemical and Bioprocesses Engineering, School of Engineering, Pontificia Universidad Católica de Chile, Vicuña Mackenna 4860, Santiago 7810000, Chile.

Nakia Polidori (N)

NAWI Graz, BioTechMed Graz, Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50, Graz 8010, Austria.

Lucija Sovic (L)

NAWI Graz, BioTechMed-Graz, Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, Graz 8010, Austria.

Daniel Kracher (D)

NAWI Graz, BioTechMed-Graz, Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, Graz 8010, Austria.

Leen Assil-Companioni (L)

NAWI Graz, BioTechMed-Graz, Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, Graz 8010, Austria.
ACIB GmbH, Petersgasse 14/1, Graz 8010, Austria.

Philipp Galuska (P)

NAWI Graz, BioTechMed Graz, Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50, Graz 8010, Austria.

Loreto P Parra (LP)

Schools of Engineering, Medicine and Biological Sciences, Institute for Biological and Medical Engineering, Pontificia Universidad Católica de Chile, Vicuña Mackenna 4860, Santiago 7810000, Chile.

Karl Gruber (K)

NAWI Graz, BioTechMed Graz, Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50, Graz 8010, Austria.

Robert Kourist (R)

NAWI Graz, BioTechMed-Graz, Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, Graz 8010, Austria.
ACIB GmbH, Petersgasse 14/1, Graz 8010, Austria.

Classifications MeSH