Increased Yield of Extracellular Vesicles after Cytochalasin B Treatment and Vortexing.
cytochalasin B-induced membrane vesicles
delivery system
extracellular vesicles
mesenchymal stem cells
Journal
Current issues in molecular biology
ISSN: 1467-3045
Titre abrégé: Curr Issues Mol Biol
Pays: Switzerland
ID NLM: 100931761
Informations de publication
Date de publication:
15 Mar 2023
15 Mar 2023
Historique:
received:
23
11
2022
revised:
07
03
2023
accepted:
13
03
2023
medline:
29
3
2023
entrez:
28
3
2023
pubmed:
29
3
2023
Statut:
epublish
Résumé
Extracellular vesicles (EVs) are promising therapeutic instruments and vectors for therapeutics delivery. In order to increase the yield of EVs, a method of inducing EVs release using cytochalasin B is being actively developed. In this work, we compared the yield of naturally occurring extracellular vesicles and cytochalasin B-induced membrane vesicles (CIMVs) from mesenchymal stem cells (MSCs). In order to maintain accuracy in the comparative analysis, the same culture was used for the isolation of EVs and CIMVs: conditioned medium was used for EVs isolation and cells were harvested for CIMVs production. The pellets obtained after centrifugation 2300×
Identifiants
pubmed: 36975528
pii: cimb45030158
doi: 10.3390/cimb45030158
pmc: PMC10047179
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2431-2443Subventions
Organisme : Russian Science Foundation
ID : №21-75-10035
Organisme : Russian Government Program "Recruitment of the Leading Scientists into the Russian Institutions of Higher Education"
ID : №075-15-2021-600
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