A rabbit model for outer retinal atrophy caused by surgical RPE removal.
Animal model
Cell therapy
Mechanical debridement
RPE degeneration
Rabbit
Subretinal surgery
Journal
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
ISSN: 1435-702X
Titre abrégé: Graefes Arch Clin Exp Ophthalmol
Pays: Germany
ID NLM: 8205248
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
received:
12
01
2023
accepted:
10
02
2023
revised:
12
01
2023
medline:
26
7
2023
pubmed:
29
3
2023
entrez:
28
3
2023
Statut:
ppublish
Résumé
We aimed to establish a rabbit model with retinal atrophy induced by an iatrogenic retinal pigment epithelium (RPE) removal, for future testing of the efficacy and safety of cell therapy strategies. A localized detachment of the retina from the RPE/choroid layer was created in 18 pigmented rabbits. The RPE was removed by scraping with a custom-made extendable loop instrument. The resulting RPE wound was observed over a time course of 12 weeks with optical coherence tomography and angiography. After 4 days (group 1) and 12 weeks (group 2), histology was done and staining with hematoxylin and eosin, as well as immunofluorescence performed to further investigate the effects of debridement on the RPE and the overlying retina. Already after 4 days, we observed a closure of the RPE wound by proliferating RPE and microglia/macrophage cells forming a multilayered clump. This pattern continued over the observation time course of 12 weeks, whereby the inner and outer nuclear layer of the retina became atrophic. No neovascularization was observed in the angiograms or histology. The observed changes were limited to the site of the former RPE wound. Localized surgical RPE removal induced an adjacent progressive retinal atrophy. Altering the natural course of this model may serve as a basis to test RPE cell therapeutics.
Identifiants
pubmed: 36976356
doi: 10.1007/s00417-023-06014-3
pii: 10.1007/s00417-023-06014-3
pmc: PMC10368565
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2265-2280Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : STA 1135/2-1
Organisme : Bundesministerium für Bildung und Forschung
ID : 01EK1613A
Informations de copyright
© 2023. The Author(s).
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