Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms.

antimicrobial activity diclofenac endodontic biofilms ibuprofen non-steroidal anti-inflammatory drugs

Journal

Antibiotics (Basel, Switzerland)
ISSN: 2079-6382
Titre abrégé: Antibiotics (Basel)
Pays: Switzerland
ID NLM: 101637404

Informations de publication

Date de publication:
23 Feb 2023
Historique:
received: 11 01 2023
revised: 14 02 2023
accepted: 20 02 2023
medline: 30 3 2023
entrez: 29 3 2023
pubmed: 30 3 2023
Statut: epublish

Résumé

Persistent infections have become a challenge in dentistry because of growing antibiotic resistance. Nonsteroidal anti-inflammatory drugs (NSAIDs) appear to be a therapeutic alternative to control biofilm infection. The objective of this work is to evaluate the antimicrobial activity and cytotoxicity of sodium diclofenac (DCS), ibuprofen (IBP) and ibuprofen arginine (IBP-arginine) solutions against endodontic polymicrobial biofilms. Sterile radicular dentin blocks of 4 mm × 4 mm × 0.7 mm were used as substrate to grow biofilm. The dentin blocks were submerged into solutions for 5 min. The antimicrobial activity was evaluated by means of the adenosine triphosphate (ATP) assay and confocal laser scanning microscopy (CLSM). Fibroblasts 3T3-L1 (ECACC 86052701) were used to test the cytotoxicity of irrigating solutions. The antibiofilm effects determined by the ATP assay showed that 4% IBP-arginine solution exerted the highest antibiofilm activity, followed by 4% DCS and 4% IBP, with statistical differences among groups (

Identifiants

pubmed: 36978317
pii: antibiotics12030450
doi: 10.3390/antibiotics12030450
pmc: PMC10044545
pii:
doi:

Types de publication

Journal Article

Langues

eng

Références

Curr Med Res Opin. 2010 Jul;26(7):1715-31
pubmed: 20470236
J Endod. 2017 Jan;43(1):131-134
pubmed: 27939738
Pharmaceutics. 2022 Aug 12;14(8):
pubmed: 36015305
J Rheumatol Suppl. 1997 Jul;49:20-4
pubmed: 9249647
Molecules. 2021 Oct 31;26(21):
pubmed: 34771014
Antibiotics (Basel). 2021 Nov 18;10(11):
pubmed: 34827350
Front Cell Infect Microbiol. 2022 Jul 22;12:908859
pubmed: 35937695
Int Endod J. 2022 May;55 Suppl 3:512-530
pubmed: 34958494
BMC Pharmacol Toxicol. 2021 Feb 4;22(1):10
pubmed: 33541432
J Bacteriol. 2016 Sep 09;198(19):2651-61
pubmed: 27161116
Clin Oral Investig. 2020 Feb;24(2):757-763
pubmed: 31139978
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005 Feb;99(2):231-52
pubmed: 15660098
Appl Environ Microbiol. 2009 Mar;75(6):1734-9
pubmed: 19139239
Arch Oral Biol. 2017 Oct;82:256-262
pubmed: 28668766
Biomimetics (Basel). 2022 Sep 25;7(4):
pubmed: 36278700
J Endod. 2021 Jul;47(7):1138-1143
pubmed: 33864882
J Oral Sci. 2020 Jun 23;62(3):285-287
pubmed: 32475864
Dent Mater J. 2019 Oct 2;38(5):701-706
pubmed: 31257302
J Dent (Tehran). 2013 Jan;10(1):16-22
pubmed: 23724199
J Endod. 2022 Jul;48(7):855-863
pubmed: 35381276
J Dent Res. 2017 Jul;96(8):924-930
pubmed: 28486080
J Dent. 2022 Jul;122:104096
pubmed: 35307476
Antibiotics (Basel). 2020 Mar 02;9(3):
pubmed: 32131427
Front Microbiol. 2021 Jul 27;12:707629
pubmed: 34385992
Int Endod J. 2021 Sep;54(9):1482-1490
pubmed: 33938010
Dent Mater J. 2017 May 31;36(3):319-324
pubmed: 28228624
Braz Dent J. 2022 May-Jun;33(3):47-54
pubmed: 35766716
Pharmaceutics. 2023 Jan 05;15(1):
pubmed: 36678827
Antibiotics (Basel). 2022 Jun 17;11(6):
pubmed: 35740222
Eur J Clin Microbiol Infect Dis. 2009 Aug;28(8):881-91
pubmed: 19399540
Drugs. 2015 May;75(8):859-77
pubmed: 25963327
Int Endod J. 2020 Jun;53(6):742-753
pubmed: 32034789
Eur J Med Chem. 2021 Apr 15;216:113293
pubmed: 33640673
J Endod. 2012 Jun;38(6):826-8
pubmed: 22595119
Eur J Med Chem. 2019 Mar 15;166:197-205
pubmed: 30711830
Caries Res. 2019;53(5):514-526
pubmed: 30947169
Int Endod J. 2023 Jan;56(1):103-117
pubmed: 36169591
Int Endod J. 2010 May;43(5):430-5
pubmed: 20518937
Commun Biol. 2022 Nov 12;5(1):1234
pubmed: 36371541
Int J Mol Sci. 2017 Aug 11;18(8):
pubmed: 28800075
Antibiotics (Basel). 2020 Sep 10;9(9):
pubmed: 32927675
Clin Oral Investig. 2022 Mar;26(3):2661-2669
pubmed: 34713359
Int J Environ Res Public Health. 2022 Dec 08;19(24):
pubmed: 36554332

Auteurs

Carmen María Ferrer-Luque (CM)

Department of Stomatology, University of Granada, 18071 Granada, Spain.
Instituto de Investigación Biosanitaria ibs.GRANADA, 18012 Granada, Spain.

Carmen Solana (C)

Instituto de Investigación Biosanitaria ibs.GRANADA, 18012 Granada, Spain.

Beatriz Aguado (B)

Independent Researcher, 18194 Granada, Spain.

Matilde Ruiz-Linares (M)

Department of Stomatology, University of Granada, 18071 Granada, Spain.
Instituto de Investigación Biosanitaria ibs.GRANADA, 18012 Granada, Spain.

Classifications MeSH