Plasma and Synovial Fluid Cell-Free DNA Concentrations Following Induction of Osteoarthritis in Horses.
biomarker
carpus
cell-free DNA
horse
osteoarthritis
plasma
synovial fluid
Journal
Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614
Informations de publication
Date de publication:
14 Mar 2023
14 Mar 2023
Historique:
received:
05
02
2023
revised:
03
03
2023
accepted:
11
03
2023
medline:
30
3
2023
entrez:
29
3
2023
pubmed:
30
3
2023
Statut:
epublish
Résumé
Biomarkers for osteoarthritis (OA) in horses have been extensively investigated, but translation into clinical use has been limited due to cost, limited sensitivity, and practicality. Identifying novel biomarkers that overcome these limitations could facilitate early diagnosis and therapy. This study aimed to compare the concentrations of synovial fluid (SF) and plasma cell-free DNA (cfDNA) over time in control horses with those with induced carpal OA. Following an established model, unilateral carpal OA was induced in 9 of 17 healthy Thoroughbred fillies, while the remainder were sham-operated controls. Synovial fluid and plasma samples were obtained before induction of OA (Day 0) and weekly thereafter until Day 63, and cfDNA concentrations were determined using fluorometry. The SF cfDNA concentrations were significantly higher for OA joints than for sham-operated joints on Days 28 (median 1430 μg/L and 631 μg/L, respectively,
Identifiants
pubmed: 36978592
pii: ani13061053
doi: 10.3390/ani13061053
pmc: PMC10044647
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : New Zealand Equine Trust, Massey University
ID : N/A
Organisme : Lewis Fitch Veterinary Research Fund, Massey University
ID : N/A
Références
Equine Vet J Suppl. 2012 Dec;(43):73-7
pubmed: 23447882
Osteoarthr Cartil Open. 2022 Aug 08;4(4):100297
pubmed: 36474792
Osteoarthritis Cartilage. 2011 May;19(5):515-42
pubmed: 21396468
Equine Vet J. 2010 Oct;42(7):643-51
pubmed: 20840580
Sports Med. 2012 Jul 1;42(7):565-86
pubmed: 22694348
Osteoarthritis Cartilage. 2008 Oct;16(10):1196-204
pubmed: 18442931
Eur J Appl Physiol. 2010 Nov;110(4):695-701
pubmed: 20577758
Equine Vet J. 2005 May;37(3):250-6
pubmed: 15892235
Theriogenology. 2012 Feb;77(3):694-8
pubmed: 22000028
Vet Rec. 1985 Jan 19;116(3):66-9
pubmed: 3976145
Equine Vet J. 1997 Sep;29(5):349-59
pubmed: 9306060
J Vet Diagn Invest. 2022 May;34(3):402-406
pubmed: 35168428
Ann Hematol. 2007 Feb;86(2):139-43
pubmed: 17024502
PLoS One. 2018 Jan 25;13(1):e0191915
pubmed: 29370268
Clin Exp Immunol. 1984 Apr;56(1):185-92
pubmed: 6609032
Cancer Treat Rev. 2002 Oct;28(5):255-71
pubmed: 12435372
Am J Vet Res. 1998 Dec;59(12):1619-28
pubmed: 9858417
Vet Surg. 2007 Jan;36(1):57-63
pubmed: 17214821
Vet Surg. 2001 Nov-Dec;30(6):528-38
pubmed: 11704948
Clin Chim Acta. 2009 Nov;409(1-2):112-6
pubmed: 19751713
Equine Vet J. 2001 Sep;33(5):478-86
pubmed: 11558743
Equine Vet J. 1982 Jul;14(3):185-98
pubmed: 7106081
Am J Vet Res. 2001 Sep;62(9):1467-77
pubmed: 11560279
Clin Epigenetics. 2019 Jun 24;11(1):93
pubmed: 31234922
Geroscience. 2023 Feb;45(1):85-103
pubmed: 35864375
Cancer Res. 1977 Mar;37(3):646-50
pubmed: 837366
Am J Vet Res. 1999 Mar;60(3):306-9
pubmed: 10188811
Equine Vet J. 2005 Sep;37(5):473-82
pubmed: 16163952
Postgrad Med J. 2014 Mar;90(1061):171-8
pubmed: 24534711
Int J Genomics. 2019 Jun 2;2019:8173630
pubmed: 31281830
Ann Clin Biochem. 2009 Nov;46(Pt 6):488-94
pubmed: 19729503
Equine Vet J. 2007 Mar;39(2):143-8
pubmed: 17378443
Eur J Appl Physiol. 2008 Nov;104(4):711-7
pubmed: 18651163
Vet Clin North Am Equine Pract. 2008 Apr;24(1):153-75
pubmed: 18314041
Cancer Res. 2001 Feb 15;61(4):1659-65
pubmed: 11245480
J Vet Intern Med. 2018 Jan;32(1):157-164
pubmed: 29230875
Equine Vet J. 2004 May;36(4):346-50
pubmed: 15163043
J Rheumatol Suppl. 2004 Apr;70:28-35
pubmed: 15132352
Vet Q. 2016 Sep;36(3):122-9
pubmed: 27103480
Clin Biochem. 2010 Nov;43(16-17):1368-70
pubmed: 20800058
PLoS One. 2021 Apr 26;16(4):e0242635
pubmed: 33901192
Vet Clin North Am Equine Pract. 2008 Aug;24(2):437-54, viii
pubmed: 18652964
C R Seances Soc Biol Fil. 1948 Feb;142(3-4):241-3
pubmed: 18875018
Anal Biochem. 2013 Feb 15;433(2):227-34
pubmed: 22750103
Vet Comp Oncol. 2007 Sep;5(3):145-55
pubmed: 19754786
Cancer Biol Ther. 2019;20(8):1057-1067
pubmed: 30990132
Int J Mol Sci. 2021 Aug 19;22(16):
pubmed: 34445645
Arthritis Rheum. 1981 Sep;24(9):1142-50
pubmed: 7306236
Clin Chem. 2007 Sep;53(9):1609-14
pubmed: 17712000
Eur J Appl Physiol. 2015 Nov;115(11):2271-80
pubmed: 26126838
Acta Vet Scand. 2019 May 30;61(1):24
pubmed: 31146775
Osteoarthritis Cartilage. 2011 Apr;19(4):346-53
pubmed: 21320614
Best Pract Res Clin Rheumatol. 2014 Feb;28(1):61-71
pubmed: 24792945
Clin Chem. 2004 Sep;50(9):1668-70
pubmed: 15331502
Am J Vet Res. 2000 Oct;61(10):1252-8
pubmed: 11039557
Vet J. 2017 Apr;222:60-67
pubmed: 28392152
Clin Chem. 2006 Sep;52(9):1820-4
pubmed: 16840584
Biol Rev Camb Philos Soc. 2018 Aug;93(3):1649-1683
pubmed: 29654714
Clin Chim Acta. 2003 Jan;327(1-2):95-101
pubmed: 12482623
Nat Commun. 2018 Nov 29;9(1):5068
pubmed: 30498206
PLoS One. 2013 Sep 30;8(9):e75485
pubmed: 24098698
Ann N Y Acad Sci. 2008 Aug;1137:18-26
pubmed: 18837919
Aging Cell. 2013 Jun;12(3):388-97
pubmed: 23438186
Clin Chem. 2011 Apr;57(4):633-6
pubmed: 21296972
J Clin Invest. 1973 Jan;52(1):198-204
pubmed: 4629907
Gene Ther. 2002 Jan;9(1):12-20
pubmed: 11850718
J Vet Intern Med. 2018 Jan;32(1):135-141
pubmed: 29197094
Osteoarthritis Cartilage. 2008 Dec;16(12):1519-25
pubmed: 18504148
Vet Clin North Am Equine Pract. 2001 Apr;17(1):115-30, vii
pubmed: 11488039
Nat Rev Clin Oncol. 2013 Jul;10(7):377-89
pubmed: 23712187
Acta Orthop Scand. 1991 Dec;62(6):623-32
pubmed: 1767663
Vet Clin North Am Equine Pract. 2001 Apr;17(1):75-94
pubmed: 11488047