Evidence of Nrf2/Keap1 Signaling Regulation by Mitochodria-Generated Reactive Oxygen Species in RGK1 Cells.

Humans Reactive Oxygen Species / metabolism NF-E2-Related Factor 2 / genetics Kelch-Like ECH-Associated Protein 1 / genetics Signal Transduction Superoxide Dismutase / genetics Apoptosis
Nrf2/Keap1 cell signaling gastric mucosal cells mitochondria reactive oxygen species signal transduction tumorized cells

Journal

Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414

Informations de publication

Date de publication:
27 02 2023
Historique:
received: 15 01 2023
revised: 15 02 2023
accepted: 16 02 2023
medline: 30 3 2023
entrez: 29 3 2023
pubmed: 30 3 2023
Statut: epublish

Résumé

It has been known that reactive oxygen species (ROS) are generated from the mitochondrial electron transport chain (ETC). Majima et al. proved that mitochondrial ROS (mtROS) caused apoptosis for the first time in 1998 (Majima et al. J Biol Chem, 1998). It is speculated that mtROS can move out of the mitochondria and initiate cellular signals in the nucleus. This paper aims to prove this phenomenon by assessing the change in the amount of manganese superoxide dismutase (MnSOD) by MnSOD transfection. Two cell lines of the same genetic background, of which generation of mtROS are different, i.e., the mtROS are more produced in RGK1, than in that of RGM1, were compared to analyze the cellular signals. The results of immunocytochemistry staining showed increase of Nrf2, Keap1, HO-1 and 2, MnSOD, GCL, GST, NQO1, GATA1, GATA3, GATA4, and GATA5 in RGK1 compared to those in RGM1. Transfection of human MnSOD in RGK1 cells showed a decrease of those signal proteins, suggesting mtROS play a role in cellular signals in nucleus.

Identifiants

DOI: 10.3390/biom13030445 PMID: 36979380 PMC: PMC10046053
pubmed: 36979380
pii: biom13030445
doi: 10.3390/biom13030445
pmc: PMC10046053
pii:
doi:

Substances chimiques

Reactive Oxygen Species 0
NF-E2-Related Factor 2 0
Kelch-Like ECH-Associated Protein 1 0
Superoxide Dismutase EC 1.15.1.1
KEAP1 protein, human 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Références

Mitochondrion. 2007 Feb-Apr;7(1-2):106-18
pubmed: 17307400
Curr Pharm Des. 2011;17(21):2190-5
pubmed: 21774784
Handb Exp Pharmacol. 2017;240:439-456
pubmed: 28176043
Biochem Cell Biol. 1992 Jun;70(6):390-403
pubmed: 1333230
Nat Immunol. 2001 Jan;2(1):45-50
pubmed: 11135577
Free Radic Biol Med. 1998 Mar 1;24(4):586-93
pubmed: 9559871
Biochem Pharmacol. 2000 Jan 1;59(1):13-23
pubmed: 10605930
Arch Biochem Biophys. 2004 Mar 1;423(1):47-56
pubmed: 14989264
FEBS Lett. 1991 Nov 18;293(1-2):199-203
pubmed: 1959661
J Clin Invest. 1996 Sep 1;98(5):1253-60
pubmed: 8787689
Sci Am. 1997 Aug;277(2):40-7
pubmed: 9245840
Science. 1956 Feb 24;123(3191):309-14
pubmed: 13298683
Int J Mol Sci. 2012;13(9):10697-10721
pubmed: 23109817
In Vitro Cell Dev Biol Anim. 2008 Jan-Feb;44(1-2):26-30
pubmed: 18034283
Mol Carcinog. 1992;6(4):238-42
pubmed: 1485915
Anal Biochem. 1971 Nov;44(1):276-87
pubmed: 4943714
J Clin Biochem Nutr. 2015 Mar;56(2):91-7
pubmed: 25759513
Antioxid Redox Signal. 2004 Jun;6(3):523-35
pubmed: 15130279
Free Radic Res. 2011 Jun;45(6):707-16
pubmed: 21486114
Biochem Pharmacol. 2000 Oct 15;60(8):1075-83
pubmed: 11007944
In Vitro Cell Dev Biol Anim. 1996 May;32(5):259-61
pubmed: 8792153
Cell Res. 2011 Jan;21(1):103-15
pubmed: 21187859
Blood. 1993 Aug 15;82(4):1212-20
pubmed: 8353285
Biochem J. 1979 Apr 15;180(1):129-35
pubmed: 39543
Biochem Biophys Res Commun. 2008 Feb 29;367(1):103-8
pubmed: 18164680
Cell. 1989 Sep 8;58(5):923-31
pubmed: 2476237
Biochim Biophys Acta. 2006 May-Jun;1757(5-6):553-61
pubmed: 16678117
FASEB J. 1993 Feb 1;7(2):361-8
pubmed: 8440412
Cell Metab. 2015 Aug 4;22(2):204-6
pubmed: 26073494
BMC Biol. 2014 May 27;12:34
pubmed: 24884669
Am J Physiol Heart Circ Physiol. 2007 May;292(5):H2023-31
pubmed: 17237240
Radiat Res. 2003 Nov;160(5):568-78
pubmed: 14565825
Cancer Res. 2001 Jul 15;61(14):5382-8
pubmed: 11454680
J Biol Chem. 2004 Nov 19;279(47):49064-73
pubmed: 15317809
Clin Breast Cancer. 2015 Feb;15(1):e55-62
pubmed: 25445418
Antioxid Redox Signal. 2009 Sep;11(9):2209-22
pubmed: 19203223
FASEB J. 1999 Jul;13(10):1137-43
pubmed: 10385605
Cancer Res. 2001 May 15;61(10):3894-901
pubmed: 11358802
J Biol Chem. 2012 Feb 10;287(7):4434-40
pubmed: 21832045
Nat Rev Mol Cell Biol. 2014 Jun;15(6):411-21
pubmed: 24854789
Cell Signal. 2007 Jul;19(7):1419-33
pubmed: 17317104
J Biol Chem. 2003 Jan 31;278(5):3170-5
pubmed: 12419811
J Biol Chem. 1992 Nov 25;267(33):23937-41
pubmed: 1385428
Free Radic Res. 2012 Aug;46(8):1029-43
pubmed: 22656864
Int J Clin Exp Pathol. 2018 Jun 01;11(6):3084-3090
pubmed: 31938435
FEBS Lett. 2002 May 22;519(1-3):231-7
pubmed: 12023051
Mol Immunol. 2008 Sep;45(15):3984-9
pubmed: 18657320
Arch Biochem Biophys. 2016 Apr 1;595:54-60
pubmed: 27095216
Cell. 2015 Oct 22;163(3):560-9
pubmed: 26496603
Radiother Oncol. 1993 Feb;26(2):177-9
pubmed: 8465019
N Engl J Med. 2003 Jun 26;348(26):2656-68
pubmed: 12826641
J Biol Chem. 1995 Jun 9;270(23):14214-9
pubmed: 7775482
Biochem J. 1973 Jul;134(3):707-16
pubmed: 4749271
J Physiol. 2021 Mar;599(6):1745-1757
pubmed: 33347611
Proc Natl Acad Sci U S A. 1997 May 13;94(10):5361-6
pubmed: 9144242
Nat Rev Drug Discov. 2018 Dec;17(12):865-886
pubmed: 30393373
Exp Ther Med. 2017 Oct;14(4):3868-3873
pubmed: 29042994
Biochem Pharmacol. 2006 Nov 30;72(11):1493-505
pubmed: 16723122
J Toxicol Environ Health. 1994 Oct;43(2):239-49
pubmed: 7932852
J Clin Biochem Nutr. 2015 Jan;56(1):1-7
pubmed: 25834301
Biomolecules. 2020 Feb 17;10(2):
pubmed: 32079324
Mitochondrion. 2007 Jun;7 Suppl:S51-61
pubmed: 17482887
J Biol Chem. 2003 Jun 27;278(26):24233-41
pubmed: 12711606
J Biol Chem. 1998 Apr 3;273(14):8217-24
pubmed: 9525927

Auteurs

Hiroko P Indo (HP)

Department of Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan.
Amanogawa Galactic Astronomy Research Center (AGARC), Graduate School of Sciences, Kagoshima University, Kagoshima 890-0065, Japan.

Daisuke Masuda (D)

Department of Space Environmental Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan.
Utilization & Engineer Department, Japan Manned Space Systems Corporation, 21-6 Tsukuba, Tsukuba 305-0047, Japan.

Sompong Sriburee (S)

Department of Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan.
Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.

Hiromu Ito (H)

Department of Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan.
Quantum RedOx Chemistry Team, Institute for Quantum Life Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, Chiba 263-8555, Japan.

Ikuo Nakanishi (I)

Quantum RedOx Chemistry Team, Institute for Quantum Life Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, Chiba 263-8555, Japan.
ORCID: 0000-0001-9204-0664

Ken-Ichiro Matsumoto (KI)

Quantitative RedOx Sensing Group, Department of Radiation Regulatory Science Research, National Institute of Radiological Sciences, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology (QST), Chiba 263-8555, Japan.
ORCID: 0000-0002-8151-4067

Samlee Mankhetkorn (S)

Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.

Moragot Chatatikun (M)

School of Allied Health Sciences, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand.
Center of Excellence Research for Melioidosis and Microorganisms, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand.
ORCID: 0000-0003-3264-1155

Sirirat Surinkaew (S)

School of Allied Health Sciences, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand.
Research Excellence Center for Innovation and Health Products (RECIHP), School of Allied Health Sciences, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand.
ORCID: 0000-0001-9161-8567

Lunla Udomwech (L)

School of Medicine, Walailak University, Thasala, Nakhon Si Thammarat 80161, Thailand.

Fumitaka Kawakami (F)

Department of Regulation Biochemistry, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara 252-0373, Japan.
Department of Health Administration, School of Allied Health Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara 252-0373, Japan.
Regenerative Medicine and Cell Design Research Facility, School of Allied Health Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara 252-0373, Japan.
ORCID: 0000-0002-8781-2516

Takafumi Ichikawa (T)

Department of Regulation Biochemistry, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara 252-0373, Japan.
Department of Health Administration, School of Allied Health Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara 252-0373, Japan.

Hirofumi Matsui (H)

Division of Gastroenterology, Graduate School of Comprehensive Human Sciences, University Tsukuba, Tsukuba 305-8575, Japan.
ORCID: 0000-0002-7659-5093

Jitbanjong Tangpong (J)

School of Allied Health Sciences, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand.
Research Excellence Center for Innovation and Health Products (RECIHP), School of Allied Health Sciences, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand.

Hideyuki J Majima (HJ)

Department of Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan.
Amanogawa Galactic Astronomy Research Center (AGARC), Graduate School of Sciences, Kagoshima University, Kagoshima 890-0065, Japan.
Department of Space Environmental Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan.
School of Allied Health Sciences, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand.
Center of Excellence Research for Melioidosis and Microorganisms, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand.
Research Excellence Center for Innovation and Health Products (RECIHP), School of Allied Health Sciences, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Evidence of Nrf2/Keap1 Signaling Regulation by...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Espèces réactives de l'oxygène › Evidence of Nrf2/Keap1 Signaling Regulation by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription à motif basique et à glissière à leucines › Facteur-2 apparenté à NF-E2 › Evidence of Nrf2/Keap1 Signaling Regulation by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines adaptatrices de la transduction du signal › Protéine-1 de type kelch associée à ECH › Evidence of Nrf2/Keap1 Signaling Regulation by...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › Evidence of Nrf2/Keap1 Signaling Regulation by...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Superoxide dismutase › Evidence of Nrf2/Keap1 Signaling Regulation by...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mort cellulaire › Mort cellulaire régulée › Apoptose › Evidence of Nrf2/Keap1 Signaling Regulation by...