Morpho-Functional Effect of a New Collagen-Based Medical Device on Human Gingival Fibroblasts: An In Vitro Study.
FAK
Hippo pathway
YAP/TAZ
collagen
gingival fibroblasts
mechanotransduction
oral soft tissue regeneration
parodontology
regenerative medicine
wound healing
Journal
Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304
Informations de publication
Date de publication:
05 Mar 2023
05 Mar 2023
Historique:
received:
03
02
2023
revised:
28
02
2023
accepted:
03
03
2023
medline:
30
3
2023
entrez:
29
3
2023
pubmed:
30
3
2023
Statut:
epublish
Résumé
Maintaining periodontal and peri-implant soft tissues health is crucial for the long-term health of teeth and dental implants. New biomedical strategies aimed at avoiding connective tissue alterations and related diseases (e.g., periodontitis and peri-implantitis) are constantly evolving. Among these, collagen-based medical products have proven to be safe and effective. Accordingly, the aim of the present study was to evaluate the effects of Dental SKIN BioRegulation (Guna S.p.a., Milan, Italy), a new injectable medical device composed of type I collagen of porcine origin, on primary cultures of human gingival fibroblasts (hGF). To this end, hGF were cultured on collagen-coated (COL, 100 µg/2 mL) or uncoated plates (CTRL) before evaluating cell viability (24 h, 48 h, 72 h, and 7 d), wound healing properties (3 h, 6 h, 12 h, 24 h, and 48 h), and the activation of mechanotransduction markers, such as FAK, YAP, and TAZ (48 h). The results proved a significant increase in cell viability at 48 h (
Identifiants
pubmed: 36979765
pii: biomedicines11030786
doi: 10.3390/biomedicines11030786
pmc: PMC10045070
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
J Funct Biomater. 2022 Aug 18;13(3):
pubmed: 35997459
J Clin Med. 2020 May 11;9(5):
pubmed: 32403382
Adv Drug Deliv Rev. 2007 Nov 10;59(13):1299-305
pubmed: 17825456
J Periodontol. 2013 Dec;84(12):1755-67
pubmed: 23451989
J Funct Biomater. 2022 Nov 16;13(4):
pubmed: 36412890
J Biol Phys. 2020 Dec;46(4):351-369
pubmed: 32860547
Ann Maxillofac Surg. 2018 Jul-Dec;8(2):218-223
pubmed: 30693235
Int J Oral Maxillofac Implants. 2013 Nov-Dec;28(6):1536-45
pubmed: 24278922
J Clin Periodontol. 2016 Mar;43(3):305-10
pubmed: 26718112
Clin Implant Dent Relat Res. 2022 Nov 29;:
pubmed: 36444772
Clin Oral Investig. 2018 Jun;22(5):2121-2128
pubmed: 29275490
Adv Drug Deliv Rev. 2018 Apr;129:407-419
pubmed: 29425770
J Biosci Bioeng. 2011 Mar;111(3):255-8
pubmed: 21288768
ACS Appl Mater Interfaces. 2015 Sep 23;7(37):20667-77
pubmed: 26322620
Nature. 1995 Oct 12;377(6549):539-44
pubmed: 7566154
Eur J Cell Biol. 2020 Sep;99(7):151123
pubmed: 33070040
Biomolecules. 2021 May 31;11(6):
pubmed: 34073044
Clin Oral Implants Res. 2012 Mar;23(3):316-24
pubmed: 22092380
J Biomed Mater Res B Appl Biomater. 2014 Apr;102(3):643-50
pubmed: 24124021
Int J Dent. 2022 Feb 24;2022:8332631
pubmed: 35251183
BMC Musculoskelet Disord. 2016 Feb 22;17:94
pubmed: 26905565
J Biomed Mater Res B Appl Biomater. 2016 May;104(4):665-75
pubmed: 26448097
Transl Res. 2021 Oct;236:17-34
pubmed: 34161876
N Engl J Med. 2015 Mar 19;372(12):1138-49
pubmed: 25785971
Nature. 2008 May 15;453(7193):314-21
pubmed: 18480812
FASEB J. 2017 Mar;31(3):937-953
pubmed: 27881487
Exp Cell Res. 2016 Apr 10;343(1):14-20
pubmed: 26519907
J Periodontal Res. 2018 Oct;53(5):727-735
pubmed: 29732558
J Clin Med. 2019 Jul 31;8(8):
pubmed: 31370168
Clin Oral Investig. 2017 Jan;21(1):255-265
pubmed: 26969500
Cells. 2018 Dec 06;7(12):
pubmed: 30563214
Oxf Med Case Reports. 2020 Nov 24;2020(11):omaa103
pubmed: 33269086
J Periodontal Res. 2014 Aug;49(4):425-36
pubmed: 23919718
Nat Protoc. 2007;2(2):329-33
pubmed: 17406593
Clin Implant Dent Relat Res. 2016 Jun;18(3):618-34
pubmed: 25873299
Materials (Basel). 2021 Sep 11;14(18):
pubmed: 34576450
Anaerobe. 2021 Aug;70:102404
pubmed: 34146701
J Biochem. 2021 Jul 3;169(5):565-573
pubmed: 33630058
Curr Med Chem. 2019;26(6):1019-1026
pubmed: 29756567
Cells. 2020 Dec 08;9(12):
pubmed: 33302563
Adv Healthc Mater. 2022 Nov 14;:e2202042
pubmed: 36377336
Life Sci. 2022 Feb 1;290:120096
pubmed: 34715138
Int J Mol Sci. 2018 Sep 11;19(9):
pubmed: 30208663
Regen Med. 2018 Jul 1;13(5):491-495
pubmed: 30062921
Biochim Biophys Acta. 2014 Jun;1838(6):1586-93
pubmed: 24480409
Compend Contin Educ Dent. 2011 Oct;32(8):24-31; quiz 32, 34
pubmed: 22073807
Physiol Rev. 2014 Oct;94(4):1287-312
pubmed: 25287865
Bioengineering (Basel). 2021 May 11;8(5):
pubmed: 34064689
Cytotherapy. 2014 Sep;16(9):1171-86
pubmed: 24934304
Clin Oral Implants Res. 2018 Mar;29 Suppl 15:32-49
pubmed: 29498129
J Cell Biol. 2014 Oct 27;207(2):283-97
pubmed: 25332161
Materials (Basel). 2017 Sep 15;10(9):
pubmed: 28914792
Int J Oral Maxillofac Implants. 2008 Mar-Apr;23(2):323-6
pubmed: 18548930
Postepy Dermatol Alergol. 2021 Jun;38(3):440-445
pubmed: 34377125
J Tissue Eng Regen Med. 2018 Feb;12(2):e1088-e1097
pubmed: 28388010
J Biomed Mater Res A. 2020 Jun;108(6):1408-1418
pubmed: 32108993
J Cell Physiol. 2020 Mar;235(3):3096-3111
pubmed: 31556111
Biomater Adv. 2022 Nov;142:213152
pubmed: 36270159
Int J Biol Sci. 2021 Sep 24;17(14):4060-4072
pubmed: 34671220
Front Physiol. 2019 Apr 24;10:270
pubmed: 31068825
Mech Ageing Dev. 2020 Jul;189:111280
pubmed: 32512018
Cell Adh Migr. 2014;8(5):440-51
pubmed: 25482647