Characterization of the Lower Airways and Oral Microbiota in Healthy Young Persons in the Community.
16S rRNA gene sequencing
bronchial brushing
bronchoalveolar lavage
healthy subjects
microbiome
microbiota
oral wash
Journal
Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304
Informations de publication
Date de publication:
10 Mar 2023
10 Mar 2023
Historique:
received:
18
01
2023
revised:
26
02
2023
accepted:
07
03
2023
medline:
30
3
2023
entrez:
29
3
2023
pubmed:
30
3
2023
Statut:
epublish
Résumé
Lower airway dysbiosis contributes to disease pathogenesis in respiratory diseases. However, little is known regarding the microbiota of lower airways or the oral cavity of healthy young persons. To address this gap, 25 healthy persons (24.3 ± 3.3 years; 52% females; no current smokers) underwent bronchoscopy during which bronchial brushing (BB) and bronchoalveolar lavage (BAL) fluid were collected. Prior to the procedure, an oral wash (OW) sample was also obtained. Microbiome analyses (16S rRNA locus) were performed (alpha- and beta-diversity, taxa annotations, and predicted functional metagenomic profiles) according to the airway compartment (BB, BAL, and OW). The greatest microbial richness was observed in OW and the lowest in BB (
Identifiants
pubmed: 36979819
pii: biomedicines11030841
doi: 10.3390/biomedicines11030841
pmc: PMC10045431
pii:
doi:
Types de publication
Journal Article
Langues
eng
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