Dissection of Antibody Responses of Gam-COVID-Vac-Vaccinated Subjects Suggests Involvement of Epitopes Outside RBD in SARS-CoV-2 Neutralization.
COVID-19
Gam-COVID-Vac
Omicron
SARS-CoV-2
antibody
epitope
molecular interaction assay
virus-neutralization
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
07 Mar 2023
07 Mar 2023
Historique:
received:
01
02
2023
revised:
28
02
2023
accepted:
01
03
2023
medline:
30
3
2023
entrez:
29
3
2023
pubmed:
30
3
2023
Statut:
epublish
Résumé
Millions of people have been vaccinated with Gam-COVID-Vac but fine specificities of induced antibodies have not been fully studied. Plasma from 12 naïve and 10 coronavirus disease 2019 (COVID-19) convalescent subjects was obtained before and after two immunizations with Gam-COVID-Vac. Antibody reactivity in the plasma samples (n = 44) was studied on a panel of micro-arrayed recombinant folded and unfolded severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins and 46 peptides spanning the spike protein (S) and by immunoglobulin G (IgG) subclass enzyme-linked immunosorbent assay (ELISA). The ability of Gam-COVID-Vac-induced antibodies to inhibit binding of the receptor-binding domain (RBD) to its receptor angiotensin converting enzyme 2 (ACE2) was investigated in a molecular interaction assay (MIA). The virus-neutralizing capacity of antibodies was studied by the pseudo-typed virus neutralization test (pVNT) for Wuhan-Hu-1 and Omicron. We found that Gam-COVID-Vac vaccination induced significant increases of IgG
Identifiants
pubmed: 36982183
pii: ijms24065104
doi: 10.3390/ijms24065104
pmc: PMC10049224
pii:
doi:
Substances chimiques
Epitopes
0
Antibodies, Neutralizing
0
Antibodies, Viral
0
Protein Subunits
0
Spike Glycoprotein, Coronavirus
0
Immunoglobulin G
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Viravaxx
ID : AG
Organisme : Megagrant of the Government of the Russian Federation
ID : 14.W03.31.0024
Organisme : Russian Science Foundation
ID : 21-15-00286
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