Effect of Ovocystatin on Amyloid β 1-42 Aggregation-In Vitro Studies.
Alzheimer’s disease
CD
TEM
ThT
amyloid beta peptide
ovocystatin
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
12 Mar 2023
12 Mar 2023
Historique:
received:
20
01
2023
revised:
07
03
2023
accepted:
08
03
2023
medline:
30
3
2023
entrez:
29
3
2023
pubmed:
30
3
2023
Statut:
epublish
Résumé
Amyloid β peptides (Aβ) aggregating in the brain have a potential neurotoxic effect and are believed to be a major cause of Alzheimer's disease (AD) development. Thus, inhibiting amyloid polypeptide aggregation seems to be a promising approach to the therapy and prevention of this neurodegenerative disease. The research presented here is directed at the determination of the inhibitory activity of ovocystatin, the cysteine protease inhibitor isolated from egg white, on Aβ42 fibril genesis in vitro. Thioflavin-T (ThT) assays, which determine the degree of aggregation of amyloid peptides based on fluorescence measurement, circular dichroism spectroscopy (CD), and transmission electron microscopy (TEM) have been used to assess the inhibition of amyloid fibril formation by ovocystatin. Amyloid beta 42 oligomer toxicity was measured using the MTT test. The results have shown that ovocystatin possesses Aβ42 anti-aggregation activity and inhibits Aβ42 oligomer toxicity in PC12 cells. The results of this work may help in the development of potential substances able to prevent or delay the process of beta-amyloid aggregation-one of the main reasons for Alzheimer's disease.
Identifiants
pubmed: 36982505
pii: ijms24065433
doi: 10.3390/ijms24065433
pmc: PMC10049317
pii:
doi:
Substances chimiques
Amyloid beta-Peptides
0
ovocystatin
0
Peptide Fragments
0
Amyloid
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministry of Education and Science in the "Regional Initiative of Excellence" program for the years 2019-2022
ID : 016/RID/2018/19
Références
J Neurochem. 2016 Oct;139 Suppl 2:237-252
pubmed: 27255958
J Struct Biol. 2005 Sep;151(3):229-38
pubmed: 16125973
Science. 2002 Jul 19;297(5580):353-6
pubmed: 12130773
Int J Mol Med. 2017 Dec;40(6):1971-1982
pubmed: 29039468
Neurogenetics. 2005 May;6(2):85-9
pubmed: 15776278
Neurosci Biobehav Rev. 2016 Sep;68:547-562
pubmed: 27328788
Biophys Chem. 2010 Jun;149(1-2):12-21
pubmed: 20456856
J Med Chem. 2009 Apr 23;52(8):2420-8
pubmed: 19317448
ACS Chem Biol. 2014 Mar 21;9(3):777-84
pubmed: 24397822
Nat Rev Neurol. 2013 Jan;9(1):25-34
pubmed: 23183882
Biochem J. 1983 Apr 1;211(1):129-38
pubmed: 6409085
J Biol Chem. 2017 Dec 22;292(51):21071-21082
pubmed: 29046353
Acta Biochim Pol. 2014;61(4):753-8
pubmed: 25180217
Front Biosci (Schol Ed). 2011 Jan 01;3(2):541-54
pubmed: 21196395
Science. 1997 Jan 31;275(5300):630-1
pubmed: 9019820
ACS Omega. 2018 Jul 31;3(7):8401-8407
pubmed: 30087945
J Mol Biol. 2006 Jul 21;360(4):882-92
pubmed: 16797587
Adv Clin Exp Med. 2017 Jul;26(4):563-569
pubmed: 28691424
J Biol Chem. 2010 Jul 30;285(31):24228-37
pubmed: 20511235
Int J Biol Macromol. 2017 Sep;102:674-693
pubmed: 28445699
Folia Biol (Krakow). 2014;62(3):187-92
pubmed: 25403072
Clin Immunol. 2000 Dec;97(3):211-20
pubmed: 11112360
J Med Food. 2010 Apr;13(2):270-7
pubmed: 20132044
Free Radic Biol Med. 2012 Feb 1;52(3):593-600
pubmed: 22172527
Nature. 1970 Aug 15;227(5259):680-5
pubmed: 5432063
Ageing Res Rev. 2016 Dec;32:22-37
pubmed: 27125852
J Mol Biol. 1993 Dec 20;234(4):1060-9
pubmed: 8263913
Mol Neurobiol. 2012 Aug;46(1):136-50
pubmed: 22552779
Scand J Clin Lab Invest. 2007;67(2):179-90
pubmed: 17365997
Eur Cytokine Netw. 2001 Jul-Sep;12(3):462-7
pubmed: 11566627
R Soc Open Sci. 2017 Jan 4;4(1):160696
pubmed: 28280572
Nat Genet. 2007 Dec;39(12):1440-2
pubmed: 18026100
Mar Drugs. 2019 Oct 23;17(11):
pubmed: 31652867
J Immunol Methods. 1983 Dec 16;65(1-2):55-63
pubmed: 6606682
Expert Rev Neurother. 2008 May;8(5):687-9
pubmed: 18457524
Molecules. 2020 Mar 06;25(5):
pubmed: 32155822
Ageing Res Rev. 2016 Dec;32:38-50
pubmed: 27333827
J Mol Recognit. 2017 Feb;30(2):
pubmed: 27714883
Dev Cell. 2015 Jun 22;33(6):690-702
pubmed: 26004511
Brain Res Rev. 2008 Nov;59(1):201-20
pubmed: 18708092
Adv Med Sci. 2019 Mar;64(1):65-71
pubmed: 30504006
Nat Rev Dis Primers. 2015 Oct 15;1:15056
pubmed: 27188934
Front Neurosci. 2020 Dec 22;14:619667
pubmed: 33414705
Cell Mol Neurobiol. 2018 Apr;38(3):579-593
pubmed: 28623429
Commun Biol. 2020 Sep 2;3(1):484
pubmed: 32879439
J Biol Chem. 2017 Jul 7;292(27):11485-11498
pubmed: 28487367
Neurobiol Aging. 2004 Sep;25(8):1033-43
pubmed: 15212828
Br J Pharmacol. 2011 Mar;162(6):1364-79
pubmed: 21133890
Anal Biochem. 1972 May;47(1):280-93
pubmed: 4624156
Acta Pharmacol Sin. 2011 May;32(5):545-51
pubmed: 21499284
Neurochem Int. 2019 Mar;124:215-224
pubmed: 30639263
Nature. 2017 Jul 12;547(7662):153-155
pubmed: 28703214
J Biol Chem. 2005 Feb 18;280(7):5892-901
pubmed: 15590663
Nucleic Acids Res. 2018 Jul 2;46(W1):W315-W322
pubmed: 29893907
Front Mol Neurosci. 2012 Jul 06;5:79
pubmed: 22783166
Rev Neurosci. 2018 Aug 28;29(6):613-627
pubmed: 29447116
J Clin Med. 2022 Apr 23;11(9):
pubmed: 35566501