Analysis of a Combined HBHA and ESAT-6-Interferon-γ-Release Assay for the Diagnosis of Tuberculous Lymphadenopathies.

ESAT-6 HBHA IGRA tuberculous lymphadenopathy

Journal

Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588

Informations de publication

Date de publication:
08 Mar 2023
Historique:
received: 20 01 2023
revised: 10 02 2023
accepted: 06 03 2023
medline: 30 3 2023
entrez: 29 3 2023
pubmed: 30 3 2023
Statut: epublish

Résumé

The incidence of tuberculosis lymphadenopathy (TBLA) is increasing, and diagnostic procedures lack sensitivity and are often highly invasive. TBLA may be asymptomatic, and differential diagnosis with other adenopathies (ADPs) is difficult. We evaluated a blood-cell interferon-γ release assay (IGRA) with two different stage-specific mycobacterial antigens for the differential diagnosis of ADP suspected of mycobacterial origin. Twenty-one patients were included and divided into three groups: (1) cervical/axillar ADP ( An IGRA profile highly suggestive of active TB (higher IFN-γ response to ESAT-6 compared to HBHA) was found for 3/6 TBLA patients from group 1, and for all the TBLA patients from groups 2 and 3, whereas this profile was not noticed in patients with a final alternative diagnosis. These results highlight the potential value of this combined HBHA/ESAT-6 IGRA as a triage test for the differential diagnosis of ADP.

Sections du résumé

BACKGROUND AND OBJECTIVES OBJECTIVE
The incidence of tuberculosis lymphadenopathy (TBLA) is increasing, and diagnostic procedures lack sensitivity and are often highly invasive. TBLA may be asymptomatic, and differential diagnosis with other adenopathies (ADPs) is difficult. We evaluated a blood-cell interferon-γ release assay (IGRA) with two different stage-specific mycobacterial antigens for the differential diagnosis of ADP suspected of mycobacterial origin.
METHODS METHODS
Twenty-one patients were included and divided into three groups: (1) cervical/axillar ADP (
RESULTS RESULTS
An IGRA profile highly suggestive of active TB (higher IFN-γ response to ESAT-6 compared to HBHA) was found for 3/6 TBLA patients from group 1, and for all the TBLA patients from groups 2 and 3, whereas this profile was not noticed in patients with a final alternative diagnosis.
CONCLUSION CONCLUSIONS
These results highlight the potential value of this combined HBHA/ESAT-6 IGRA as a triage test for the differential diagnosis of ADP.

Identifiants

pubmed: 36983128
pii: jcm12062127
doi: 10.3390/jcm12062127
pmc: PMC10052338
pii:
doi:

Types de publication

Journal Article

Langues

eng

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Auteurs

Françoise Mascart (F)

Laboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles (ULB), 1070 Brussels, Belgium.

Maya Hites (M)

Clinic of Infectious and Tropical Diseases, Hôpital Universitaire de Bruxelles (HUB)-Hôpital Erasme, Université Libre de Bruxelles (ULB), 1070 Brussels, Belgium.

Emmanuelle Watelet (E)

Department of Pneumology, Clinique St-Anne/St-Remi-Chirec, 1070 Brussels, Belgium.

Gil Verschelden (G)

Department of Internal Medicine, Universitair Ziekenhuis Brussel-UZ Brussel, Vrije Universiteit Brussel (VUB), 1090 Brussels, Belgium.

Christelle Meuris (C)

Department of Infectious Diseases, Liège University Hospital, 4000 Liège, Belgium.

Jean-Luc Doyen (JL)

Department of Pneumology, Clinique St-Anne/St-Remi-Chirec, 1070 Brussels, Belgium.

Anne Van Praet (A)

Laboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles (ULB), 1070 Brussels, Belgium.

Audrey Godefroid (A)

Laboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles (ULB), 1070 Brussels, Belgium.

Emmanuelle Petit (E)

U-1019-UMR8204, Center for Infection and Immunity of Lille (CIIL), CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, University of Lille, 59000 Lille, France.

Mahavir Singh (M)

Lionex Diagnostics and Therapeutics, 38126 Braunschweig, Germany.

Camille Locht (C)

U-1019-UMR8204, Center for Infection and Immunity of Lille (CIIL), CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, University of Lille, 59000 Lille, France.

Véronique Corbière (V)

Laboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles (ULB), 1070 Brussels, Belgium.

Classifications MeSH