Structural and Functional Characterization of the Newly Designed Antimicrobial Peptide Crabrolin21.

CD spectroscopy NMR spectroscopy antimicrobial resistance membrane specificity

Journal

Membranes
ISSN: 2077-0375
Titre abrégé: Membranes (Basel)
Pays: Switzerland
ID NLM: 101577807

Informations de publication

Date de publication:
22 Mar 2023
Historique:
received: 02 02 2023
revised: 16 03 2023
accepted: 17 03 2023
medline: 30 3 2023
entrez: 29 3 2023
pubmed: 30 3 2023
Statut: epublish

Résumé

(1) Background: antimicrobial resistance is becoming a dramatic problem for public health, and the design of new antimicrobial agents is an active research area. (2) Methods: based on our previous work, we designed an improved version of the crabrolin peptide and characterized its functional and structural properties with a wide range of techniques. (3) Results: the newly designed peptide, crabrolin21, is much more active than the previous ones and shows specific selectivity towards bacterial cells. (4) Conclusions: crabrolin21 shows interesting properties and deserves further studies.

Identifiants

pubmed: 36984752
pii: membranes13030365
doi: 10.3390/membranes13030365
pmc: PMC10053045
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : iNEXT-Discovery in Horizon 2020
ID : 871037
Organisme : Italian Ministry of University and Research
ID : PRIN project 2020833Y75
Organisme : Regione Lazio
ID : Contributo premiale per i ricercatori 2022', DE G05411

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Auteurs

Francesca Cantini (F)

Magnetic Resonance Center (CERM), University of Florence, Sesto Fiorentino, 50019 Firenze, Italy.
Department of Chemistry, University of Florence, Sesto Fiorentino, 50019 Firenze, Italy.

Paola Giannì (P)

Department of Chemical Sciences and Technology, University of Rome Tor Vergata, 00133 Rome, Italy.
Fondazione G.I.M.EM.A.-Franco Mandelli Onlus, 00182 Rome, Italy.

Sara Bobone (S)

Department of Chemical Sciences and Technology, University of Rome Tor Vergata, 00133 Rome, Italy.

Cassandra Troiano (C)

Department of Chemical Sciences and Technology, University of Rome Tor Vergata, 00133 Rome, Italy.

Hugo van Ingen (H)

NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.

Renato Massoud (R)

Department of Experimental Medicine, University of Rome Tor Vergata, Viale della Ricerca Scientifica, 00133 Rome, Italy.

Nicoletta Perini (N)

Department of Biology, University of Rome Tor Vergata, 00133 Rome, Italy.

Luciana Migliore (L)

Department of Biology, University of Rome Tor Vergata, 00133 Rome, Italy.
eCampus University, 22060 Novedrate, Italy.

Philippe Savarin (P)

Chemistry Structures Properties of Biomaterials and Therapeutic Agents Laboratory (CSPBAT), Nanomédecine Biomarqueurs Détection Team (NBD), Sorbonne Paris Nord University, The National Center for Scientific Research (CNRS), UMR 7244, 74 Rue Marcel Cachin, CEDEX, 93017 Bobigny, France.

Charles Sanders (C)

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Center for Structural Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.

Lorenzo Stella (L)

Department of Chemical Sciences and Technology, University of Rome Tor Vergata, 00133 Rome, Italy.

Marco Sette (M)

Department of Chemical Sciences and Technology, University of Rome Tor Vergata, 00133 Rome, Italy.
Chemistry Structures Properties of Biomaterials and Therapeutic Agents Laboratory (CSPBAT), Nanomédecine Biomarqueurs Détection Team (NBD), Sorbonne Paris Nord University, The National Center for Scientific Research (CNRS), UMR 7244, 74 Rue Marcel Cachin, CEDEX, 93017 Bobigny, France.

Classifications MeSH