Protein Synthesis Determined from Non-Radioactive Phenylalanine Incorporated by Antarctic Fish.
13C-phenylalanine
isotopic labelling
polar fish
slow metabolism
somatic growth
Journal
Metabolites
ISSN: 2218-1989
Titre abrégé: Metabolites
Pays: Switzerland
ID NLM: 101578790
Informations de publication
Date de publication:
24 Feb 2023
24 Feb 2023
Historique:
received:
08
02
2023
revised:
22
02
2023
accepted:
23
02
2023
medline:
30
3
2023
entrez:
29
3
2023
pubmed:
30
3
2023
Statut:
epublish
Résumé
Direct measurements of temperature-dependent weight gains are experimentally challenging and time-consuming in long-lived/slow-growing organisms such as Antarctic fish. Here, we reassess methodology to quantify the in vivo protein synthesis rate from amino acids, as a key component of growth. We tested whether it is possible to avoid hazardous radioactive materials and whether the analytical pathway chosen is robust against analytical errors. In the eelpout,
Identifiants
pubmed: 36984778
pii: metabo13030338
doi: 10.3390/metabo13030338
pmc: PMC10051348
pii:
doi:
Types de publication
Journal Article
Langues
eng
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