Productive HIV-1 infection of tissue macrophages by fusion with infected CD4+ T cells.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
01 05 2023
01 05 2023
Historique:
received:
20
05
2022
revised:
05
12
2022
accepted:
02
02
2023
medline:
31
3
2023
entrez:
29
3
2023
pubmed:
30
3
2023
Statut:
ppublish
Résumé
Macrophages are essential for HIV-1 pathogenesis and represent major viral reservoirs. Therefore, it is critical to understand macrophage infection, especially in tissue macrophages, which are widely infected in vivo, but poorly permissive to cell-free infection. Although cell-to-cell transmission of HIV-1 is a determinant mode of macrophage infection in vivo, how HIV-1 transfers toward macrophages remains elusive. Here, we demonstrate that fusion of infected CD4+ T lymphocytes with human macrophages leads to their efficient and productive infection. Importantly, several tissue macrophage populations undergo this heterotypic cell fusion, including synovial, placental, lung alveolar, and tonsil macrophages. We also find that this mode of infection is modulated by the macrophage polarization state. This fusion process engages a specific short-lived adhesion structure and is controlled by the CD81 tetraspanin, which activates RhoA/ROCK-dependent actomyosin contractility in macrophages. Our study provides important insights into the mechanisms underlying infection of tissue-resident macrophages, and establishment of persistent cellular reservoirs in patients.
Identifiants
pubmed: 36988579
pii: 213978
doi: 10.1083/jcb.202205103
pmc: PMC10067447
pii:
doi:
Substances chimiques
CD81 protein, human
0
Actomyosin
9013-26-7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2023 Mascarau et al.
Références
Viruses. 2020 Apr 28;12(5):
pubmed: 32354203
Mucosal Immunol. 2016 May;9(3):634-46
pubmed: 26349662
PLoS Biol. 2012;10(12):e1001448
pubmed: 23271952
J Vis Exp. 2019 Jun 23;(148):
pubmed: 31282892
Sci Rep. 2021 May 11;11(1):10011
pubmed: 33976357
Sci Rep. 2012;2:763
pubmed: 23094138
J Leukoc Biol. 2022 Nov;112(5):1329-1342
pubmed: 35588259
Front Immunol. 2011 Sep 15;2:43
pubmed: 22566833
Nat Med. 1995 Dec;1(12):1320-2
pubmed: 7489416
Curr Opin Infect Dis. 2016 Jun;29(3):248-55
pubmed: 27027245
Viruses. 2011 Sep;3(9):1757-76
pubmed: 21994805
Cell Host Microbe. 2014 Dec 10;16(6):711-21
pubmed: 25467409
Cell Rep. 2019 Mar 26;26(13):3586-3599.e7
pubmed: 30917314
Cell Chem Biol. 2019 Nov 21;26(11):1544-1558.e6
pubmed: 31522999
Proc Natl Acad Sci U S A. 2020 May 5;117(18):9981-9990
pubmed: 32300019
Cell Adh Migr. 2014;8(3):179-91
pubmed: 24713854
J Exp Med. 2004 Jan 19;199(2):283-93
pubmed: 14734528
J Cell Sci. 2008 Nov 1;121(Pt 21):3619-28
pubmed: 18946025
Front Immunol. 2018 Feb 19;9:260
pubmed: 29515578
Mol Biol Cell. 2006 Oct;17(10):4435-45
pubmed: 16855019
J Virol. 2007 Dec;81(24):13916-21
pubmed: 17913807
Methods Mol Biol. 2022;2407:205-213
pubmed: 34985667
Methods Mol Biol. 2020;2142:235-249
pubmed: 32367371
Immunity. 2014 Sep 18;41(3):493-502
pubmed: 25238099
Immunity. 2020 Jun 16;52(6):957-970
pubmed: 32553181
Blood. 2011 Nov 3;118(18):4841-52
pubmed: 21562048
Nat Rev Mol Cell Biol. 2005 Oct;6(10):801-11
pubmed: 16314869
J Immunol. 2009 May 15;182(10):6237-46
pubmed: 19414777
Proc Natl Acad Sci U S A. 2018 Mar 13;115(11):E2556-E2565
pubmed: 29463701
J Virol. 2017 Nov 30;91(24):
pubmed: 28978713
Retrovirology. 2017 Mar 9;14(1):17
pubmed: 28279181
J Infect Dis. 2000 Jul;182(1):338-42
pubmed: 10882620
Methods Enzymol. 2006;406:128-40
pubmed: 16472655
EMBO J. 2001 Jan 15;20(1-2):12-8
pubmed: 11226150
Annu Rev Physiol. 2017 Feb 10;79:541-566
pubmed: 27813830
Nat Rev Microbiol. 2008 Nov;6(11):815-26
pubmed: 18923409
Cell Mol Life Sci. 2021 Sep;78(17-18):6087-6104
pubmed: 34296319
PLoS Pathog. 2022 May 27;18(5):e1010335
pubmed: 35622876
J Leukoc Biol. 2018 Mar;103(3):421-431
pubmed: 29389021
Small GTPases. 2014;5:e29846
pubmed: 25010901
J Virol. 2014 Mar;88(6):3202-12
pubmed: 24390339
PLoS Pathog. 2017 Feb 2;13(2):e1006099
pubmed: 28152024
Sci Rep. 2016 Oct 19;6:35296
pubmed: 27759009
J Vis Exp. 2020 Jun 14;(160):
pubmed: 32597837
Nat Med. 2017 May;23(5):638-643
pubmed: 28414330
ACS Nano. 2017 Apr 25;11(4):4028-4040
pubmed: 28355484
Cell Host Microbe. 2021 Mar 10;29(3):435-447.e9
pubmed: 33571449
Nat Microbiol. 2019 Apr;4(4):633-644
pubmed: 30718846
J Leukoc Biol. 2010 Apr;87(4):599-608
pubmed: 20042468
J Cell Biol. 2003 Jun 9;161(5):945-56
pubmed: 12796480
Cell. 2016 Feb 11;164(4):695-709
pubmed: 26830877
Cell Host Microbe. 2009 Aug 20;6(2):174-86
pubmed: 19683683
J Virol. 2009 Aug;83(15):7467-74
pubmed: 19458002
JCI Insight. 2017 Feb 23;2(4):e91214
pubmed: 28239657
J Virol. 2016 Aug 12;90(17):7596-606
pubmed: 27307568
Elife. 2020 Mar 30;9:
pubmed: 32223897
Mucosal Immunol. 2014 Sep;7(5):1116-26
pubmed: 24472847
J Biol Chem. 2008 Sep 19;283(38):26089-97
pubmed: 18662991
J Virol. 2007 Jun;81(11):5547-60
pubmed: 17360745
Sci Rep. 2021 Feb 16;11(1):3890
pubmed: 33594125
Blood. 2015 Mar 5;125(10):1611-22
pubmed: 25527710
Nat Commun. 2012;3:1320
pubmed: 23271654
Front Microbiol. 2021 Apr 07;12:646447
pubmed: 33897659
Proc Natl Acad Sci U S A. 2017 Jan 24;114(4):E540-E549
pubmed: 28069953
J Virol. 2006 Jul;80(13):6487-96
pubmed: 16775336
J Virol. 2007 Aug;81(15):7873-84
pubmed: 17522207
Exp Cell Res. 2017 Mar 1;352(1):9-19
pubmed: 28132881
mBio. 2019 Nov 19;10(6):
pubmed: 31744918
Nat Cell Biol. 2008 Feb;10(2):211-9
pubmed: 18193035
J Immunol. 2006 Oct 15;177(8):5129-37
pubmed: 17015697
Cytokine. 2020 Mar;127:154939
pubmed: 31786501
J Virol. 2015 Jan 15;89(2):1329-39
pubmed: 25392215
Vaccine. 2013 Dec 2;31(49):5789-97
pubmed: 24140477
Proc Natl Acad Sci U S A. 2010 Dec 7;107(49):21016-21
pubmed: 21081699
Front Immunol. 2017 Nov 30;8:1698
pubmed: 29250073
Nature. 2012 Oct 11;490(7419):283-7
pubmed: 22854780
Nat Immunol. 2018 May;19(5):475-486
pubmed: 29670239
mBio. 2017 Aug 15;8(4):
pubmed: 28811349
Viruses. 2019 Nov 20;11(12):
pubmed: 31757023
Front Med (Lausanne). 2021 Jul 22;8:674645
pubmed: 34368184
Cell Host Microbe. 2016 Mar 9;19(3):304-10
pubmed: 26962941
J Immunol. 2010 Jun 15;184(12):7030-9
pubmed: 20488787