Two induced pluripotent stem cell (iPSC) lines derived from patients affected by Waardenburg syndrome type 1 retain potential to activate neural crest markers.


Journal

Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957

Informations de publication

Date de publication:
06 2023
Historique:
received: 18 08 2022
revised: 27 02 2023
accepted: 16 03 2023
medline: 2 6 2023
pubmed: 30 3 2023
entrez: 29 3 2023
Statut: ppublish

Résumé

Waardenburg syndrome type 1 (WS1), a rare genetic disease characterized by pigmentation defects and mild craniofacial anomalies often associated with congenital deafness is caused by heterozygous mutations in the PAX3 gene (2q36.1). We have generated two induced pluripotent stem cell lines (PCli029-A and PCli031-A) from two patients from the same family both carrying the same heterozygous deletion in PAX3 exon 1 (c.-70_85 + 366del). These cells are pluripotent as they can differentiate into ectoderm, mesoderm and endoderm. They also can activate the early neural crest marker SNAI2. These cells will be useful for studying the human neural crest-derived pigment cells.

Identifiants

pubmed: 36989619
pii: S1873-5061(23)00060-0
doi: 10.1016/j.scr.2023.103074
pmc: PMC10240564
pii:
doi:

Substances chimiques

PAX3 Transcription Factor 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

103074

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Brigitte Ontoniente reports a relationship with Phenocell SAS that includes: board membership and employment.

Références

Hum Mutat. 2010 Apr;31(4):391-406
pubmed: 20127975
Development. 2019 Aug 29;146(16):
pubmed: 31399472

Auteurs

Mansour Alkobtawi (M)

Université Paris-Saclay, CNRS UMR 3347, INSERM U1021, 91405 Orsay, France; Institut Curie Research Division, PSL Research University, rue Henri Becquerel, 91405 Orsay, France.

Patrick Pla (P)

Université Paris-Saclay, CNRS UMR 3347, INSERM U1021, 91405 Orsay, France; Institut Curie Research Division, PSL Research University, rue Henri Becquerel, 91405 Orsay, France.

Brigitte Onteniente (B)

Phenocell SAS, 45 Boulevard Marcel Pagnol, 06130 Grasse, France.

Subham Seal (S)

Université Paris-Saclay, CNRS UMR 3347, INSERM U1021, 91405 Orsay, France; Institut Curie Research Division, PSL Research University, rue Henri Becquerel, 91405 Orsay, France.

Véronique Pingault (V)

Université de Paris, INSERM, Imagine Institute, UMR 1163, Laboratory of Embryology and Genetics of Human Malformation, F-75015 Paris, France; Fédération de Génétique, Service de Génétique des Maladies rares, AP-HP, Hôpital Necker-Enfants-Malades, 149 rue de Sèvres, F-75015 Paris, France.

Sandrine Marlin (S)

Université de Paris, INSERM, Imagine Institute, UMR 1163, Laboratory of Embryology and Genetics of Human Malformation, F-75015 Paris, France; Fédération de Génétique et Médecine Génomique, Service de Médecine Génomique des Maladies Rares, UF Développement Morphogenèse, Centre de référence des surdités génétiques, Hôpital Necker-Enfants Malades, AP-HP, 75015 Paris, France.

Anne H Monsoro-Burq (AH)

Université Paris-Saclay, CNRS UMR 3347, INSERM U1021, 91405 Orsay, France; Institut Curie Research Division, PSL Research University, rue Henri Becquerel, 91405 Orsay, France; Institut Universitaire de France, 75005 Paris, France. Electronic address: anne-helene.monsoro-burq@curie.fr.

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Classifications MeSH