Pathogenic role of Twist-1 protein in hydatidiform molar pregnancies and investigation of its potential diagnostic utility in complete moles.
Complete mole
Diagnosis
Hydatidiform mole
Immunohistochemistry
Partial mole
Twist-1
Journal
Diagnostic pathology
ISSN: 1746-1596
Titre abrégé: Diagn Pathol
Pays: England
ID NLM: 101251558
Informations de publication
Date de publication:
29 Mar 2023
29 Mar 2023
Historique:
received:
31
12
2022
accepted:
21
03
2023
medline:
31
3
2023
entrez:
29
3
2023
pubmed:
30
3
2023
Statut:
epublish
Résumé
Complete and partial moles (PM) are the most common gestational trophoblastic diseases. Due to some overlapping morphological findings, ancillary studies may be necessary. In this cross-sectional study, 47 cases of complete mole (CM) and 40 cases of PM were randomly selected based on histopathological criteria. Only those cases that were agreed upon by two expert gynecological pathologists and confirmed by the P57 IHC study were included. The expression level of the Twist-1 marker in villi stromal cells, as well as syncytiotrophoblasts, was evaluated quantitatively (percentage of positive cells), qualitatively (staining intensity) and as a total comprehensive score. Expression of Twist-1 is higher and more intense in villous stromal cells of CMs (p < 0.001). Moderate to strong staining intensity in more than 50% of villous stromal cells, can differentiate CM and PM with 89.5% sensitivity and 75% specificity. In syncytiotrophoblasts of CM, Twist-1 expression was significantly lower than PM (p < 0.001). Negative or weak staining intensity in less than 10% of syncytiotrophoblasts, can distinguish CM and PM with 82.9% sensitivity and 60% specificity. A higher expression of Twist-1 in villous stromal cells of hydatidiform moles is a sensitive and specific marker for the diagnosis of CMs. An elevated expression of this marker in villous stromal cells suggests another pathogenic mechanism for more aggressiveness of CMs in addition to the characteristics of trophoblast cells. The opposite result was obtained in the expression of Twist-1 in the syncytiotrophoblasts, compatible with defects in the process of formation of these supportive cells in CMs.
Sections du résumé
BACKGROUND
BACKGROUND
Complete and partial moles (PM) are the most common gestational trophoblastic diseases. Due to some overlapping morphological findings, ancillary studies may be necessary.
METHODS
METHODS
In this cross-sectional study, 47 cases of complete mole (CM) and 40 cases of PM were randomly selected based on histopathological criteria. Only those cases that were agreed upon by two expert gynecological pathologists and confirmed by the P57 IHC study were included. The expression level of the Twist-1 marker in villi stromal cells, as well as syncytiotrophoblasts, was evaluated quantitatively (percentage of positive cells), qualitatively (staining intensity) and as a total comprehensive score.
RESULTS
RESULTS
Expression of Twist-1 is higher and more intense in villous stromal cells of CMs (p < 0.001). Moderate to strong staining intensity in more than 50% of villous stromal cells, can differentiate CM and PM with 89.5% sensitivity and 75% specificity. In syncytiotrophoblasts of CM, Twist-1 expression was significantly lower than PM (p < 0.001). Negative or weak staining intensity in less than 10% of syncytiotrophoblasts, can distinguish CM and PM with 82.9% sensitivity and 60% specificity.
CONCLUSION
CONCLUSIONS
A higher expression of Twist-1 in villous stromal cells of hydatidiform moles is a sensitive and specific marker for the diagnosis of CMs. An elevated expression of this marker in villous stromal cells suggests another pathogenic mechanism for more aggressiveness of CMs in addition to the characteristics of trophoblast cells. The opposite result was obtained in the expression of Twist-1 in the syncytiotrophoblasts, compatible with defects in the process of formation of these supportive cells in CMs.
Identifiants
pubmed: 36991485
doi: 10.1186/s13000-023-01329-5
pii: 10.1186/s13000-023-01329-5
pmc: PMC10053139
doi:
Substances chimiques
Cyclin-Dependent Kinase Inhibitor p57
0
TWIST1 protein, human
0
Twist-Related Protein 1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
40Informations de copyright
© 2023. The Author(s).
Références
Am J Surg Pathol. 2001 Oct;25(10):1225-30
pubmed: 11688455
Am J Surg Pathol. 2005 Jul;29(7):942-7
pubmed: 15958860
Epigenomics. 2021 Oct;13(19):1571-1585
pubmed: 34607487
J Reprod Med. 2004 Aug;49(8):630-6
pubmed: 15457853
Mod Pathol. 2014 Feb;27(2):238-54
pubmed: 23887308
Int J Clin Oncol. 2018 Apr;23(2):321-328
pubmed: 29101499
Methods Mol Biol. 2018;1710:165-171
pubmed: 29197002
Reprod Sci. 2008 Apr;15(4):382-93
pubmed: 18497345
Ann Diagn Pathol. 2021 Aug;53:151769
pubmed: 34146830
APMIS. 2018 Jul;126(7):647-654
pubmed: 30129126
Placenta. 2015 Nov;36(11):1318-24
pubmed: 26459371
Placenta. 2016 Mar;39:45-54
pubmed: 26992674
Iran J Reprod Med. 2015 Jul;13(7):413-20
pubmed: 26494988
Expert Rev Mol Diagn. 2022 Aug;22(8):783-796
pubmed: 36017690
Annu Rev Pathol. 2017 Jan 24;12:449-485
pubmed: 28135560
Am J Clin Pathol. 2018 Mar 29;149(5):442-455
pubmed: 29562309
Malays J Pathol. 2019 Apr;41(1):15-24
pubmed: 31025633
Microb Pathog. 2022 Jan;162:105304
pubmed: 34818576
Medicine (Baltimore). 2020 Nov 25;99(48):e23397
pubmed: 33235117
Syst Rev. 2016 Oct 4;5(1):169
pubmed: 27716354
Am J Obstet Gynecol. 2010 Dec;203(6):531-9
pubmed: 20728069
Placenta. 2010 Sep;31(9):747-55
pubmed: 20659767
Placenta. 2020 Aug;97:65-67
pubmed: 32792066
Chang Gung Med J. 2011 May-Jun;34(3):229-38
pubmed: 21733352
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2021 Feb 10;38(2):117-122
pubmed: 33565061
Pathol Res Pract. 2022 Sep;237:154041
pubmed: 35961058
Arch Pathol Lab Med. 2018 Dec;142(12):1485-1502
pubmed: 30500280
Am J Reprod Immunol. 2017 Aug;78(2):
pubmed: 28337825