Can beta-lactamase resistance genes in anaerobic Gram-negative gut bacteria transfer to gut aerobes?
Journal
The Journal of antibiotics
ISSN: 1881-1469
Titre abrégé: J Antibiot (Tokyo)
Pays: England
ID NLM: 0151115
Informations de publication
Date de publication:
06 2023
06 2023
Historique:
received:
10
09
2022
accepted:
26
02
2023
revised:
20
01
2023
medline:
30
5
2023
pubmed:
31
3
2023
entrez:
30
3
2023
Statut:
ppublish
Résumé
The study was conceived with the hypothesis that human aerobic gut flora could act as a reservoir of ß-lactamases and contribute to the emergence of ß-lactam resistance by transferring ß-lactamase genes to resident anaerobes. Thus, we studied the repertoire of ß-lactam resistance determinants (ß-lactamases associated with aerobes and anaerobes) in Gram-negative anaerobes. The phenotypic resistance against ß-lactams and the presence of aerobic and anaerobic ß-lactamases were tested in Gram-negative anaerobic isolates (n = 200) by agar dilution method and targeted PCR, respectively. In addition, whole-genome sequencing (WGS) was used to study the ß-lactam resistance determinants in 4/200 multi-drug resistant (MDR) strains. The resistance to ß-lactams was as follows: imipenem (0.5%), cefoxitin (26.5%), and piperacillin-tazobactam (27.5%). None of the isolates showed the presence of ß-lactamases found in aerobic microorganisms. The presence of anaerobic ß-lactamase genes viz. cfiA, cepA, cfxA, cfiA
Identifiants
pubmed: 36997726
doi: 10.1038/s41429-023-00608-z
pii: 10.1038/s41429-023-00608-z
doi:
Substances chimiques
beta-Lactamases
EC 3.5.2.6
Imipenem
71OTZ9ZE0A
Anti-Bacterial Agents
0
Banques de données
figshare
['10.6084/m9.figshare.20267595']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
355-359Informations de copyright
© 2023. The Author(s), under exclusive licence to the Japan Antibiotics Research Association.
Références
Smillie CS, Smith MB, Friedman J, Cordero OX, David LA, Alm EJ. Ecology drives a global network of gene exchange connecting the human microbiome. Nature. 2011;480:241–4.
doi: 10.1038/nature10571
pubmed: 22037308
Hedberg M, Nord CE. Beta-lactam resistance in anaerobic bacteria: a review. J Chemother. 1996;8:3–16.
doi: 10.1179/joc.1996.8.1.3
pubmed: 8835102
Sood A, Ray P, Angrup A. Phenotypic and genotypic antimicrobial resistance in clinical anaerobic isolates from India. JAC Antimicrob Resist. 2021;3:1–9.
doi: 10.1093/jacamr/dlab044
Angrup A, Sood A, Ray P, Bala K. Clinical anaerobic infections in an Indian tertiary care hospital: a two-year retrospective study. Anaerobe. 2021;73:102482.
doi: 10.1016/j.anaerobe.2021.102482
pubmed: 34861364
Sood A, Angrup A, Ray P, Bala K. Comparative evaluation of agar dilution and broth microdilution by commercial and in-house plates for Bacteroides fragilis group: an economical and expeditious approach for resource-limited settings. Anaerobe. 2021;71:102443.
doi: 10.1016/j.anaerobe.2021.102443
pubmed: 34492368
Sood A, Ray P, Angrup A. Antimicrobial susceptibility testing of anaerobic bacteria: in routine and research. Anaerobe. 2022;75:102559.
doi: 10.1016/j.anaerobe.2022.102559
pubmed: 35417767
Dallenne C, da Costa A, Decré D, Favier C, Arlet G. Development of a set of multiplex PCR assays for the detection of genes encoding important β-lactamases in Enterobacteriaceae. J Antimicrob Chemother. 2010;65:490–5.
doi: 10.1093/jac/dkp498
pubmed: 20071363
Aziz RK, et al. The RAST Server: rapid annotations using subsystems technology. BMC Genomics. 2008;9:75.
doi: 10.1186/1471-2164-9-75
pubmed: 18261238
pmcid: 2265698
Alcock BP, et al. CARD 2020: antibiotic resistome surveillance with the comprehensive antibiotic resistance database. Nucleic Acids Res. 2020;48:D517–25.
pubmed: 31665441
Bortolaia V, et al. ResFinder 4.0 for predictions of phenotypes from genotypes. J Antimicrob Chemother. 2020;75:3491–500.
doi: 10.1093/jac/dkaa345
pubmed: 32780112
pmcid: 7662176
Guiney DG, Hasegawa P, Davis CE. Plasmid transfer from Escherichia coli to Bacteroides fragilis: differential expression of antibiotic resistance phenotypes. Proc Natl Acad Sci USA. 1984;81:7203–6.
doi: 10.1073/pnas.81.22.7203
pubmed: 6095273
pmcid: 392106
SJ G, et al. Genome streamlining in a cosmopolitan oceanic bacterium. Science. 2005;309:1242–5.
doi: 10.1126/science.1114057
Jain R, Rivera MC, Moore JE, Lake JA. Horizontal gene transfer accelerates genome innovation and evolution. Mol Biol Evol. 2003;20:1598–602.
doi: 10.1093/molbev/msg154
pubmed: 12777514
Roux D, et al. Fitness cost of antibiotic susceptibility during bacterial infection. Sci Transl Med. 2015;7:297ra114.
Nakano V, Nascimento e Silva AD, Merino VRC, Wexler HM, Avila-Campos MJ. Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains. Clinics. 2011;66:543–7.
doi: 10.1590/S1807-59322011000400004
pubmed: 21655744
pmcid: 3093783
Kato N, Yamazoe K, Han CG, Ohtsubo E. New insertion sequence elements in the upstream region of cfiA in imipenem-resistant Bacteroides fragilis strains. Antimicrob Agents Chemother. 2003;47:979–85.
doi: 10.1128/AAC.47.3.979-985.2003
Veloo ACM, Baas WH, Haan FJ, Coco J, Rossen JW. Prevalence of antimicrobial resistance genes in Bacteroides spp. and Prevotella spp. Dutch clinical isolates. Clin Microbiol Infect. 2019;25:1156.e9–13.
doi: 10.1016/j.cmi.2019.02.017
pubmed: 30802650