Fc-mediated pan-sarbecovirus protection after alphavirus vector vaccination.

CP: Immunology FcR effector function animal model antibody responses coronavirus correlates of protection cross-protection pan-coronavirus vaccine sarbecovirus systems serology vaccine development

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
25 04 2023
Historique:
received: 18 10 2022
revised: 21 12 2022
accepted: 17 03 2023
medline: 4 10 2023
pubmed: 1 4 2023
entrez: 31 3 2023
Statut: ppublish

Résumé

Group 2B β-coronaviruses (sarbecoviruses) have caused regional and global epidemics in modern history. Here, we evaluate the mechanisms of cross-sarbecovirus protective immunity, currently less clear yet important for pan-sarbecovirus vaccine development, using a panel of alphavirus-vectored vaccines covering bat to human strains. While vaccination does not prevent virus replication, it protects against lethal heterologous disease outcomes in both severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and clade 2 bat sarbecovirus challenge models. The spike vaccines tested primarily elicit a highly S1-specific homologous neutralizing antibody response with no detectable cross-virus neutralization. Rather, non-neutralizing antibody functions, mechanistically linked to FcgR4 and spike S2, mediate cross-protection in wild-type mice. Protection is lost in FcR knockout mice, further supporting a model for non-neutralizing, protective antibodies. These data highlight the importance of FcR-mediated cross-protective immune responses in universal pan-sarbecovirus vaccine designs.

Identifiants

pubmed: 37000623
pii: S2211-1247(23)00337-6
doi: 10.1016/j.celrep.2023.112326
pmc: PMC10063157
pii:
doi:

Substances chimiques

Viral Vaccines 0
Antibodies, Viral 0
Antibodies, Neutralizing 0

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

112326

Subventions

Organisme : NIAID NIH HHS
ID : U19 AI135995
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007419
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI146785
Pays : United States
Organisme : NCI NIH HHS
ID : U54 CA260543
Pays : United States
Organisme : NIAID NIH HHS
ID : 75N93019C00052
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA260476
Pays : United States
Organisme : NIAID NIH HHS
ID : R37 AI080289
Pays : United States
Organisme : NIH HHS
ID : K01 OD026529
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI042790
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI165072
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI158571
Pays : United States

Commentaires et corrections

Type : UpdateOf

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests G.A. is a founder/equity holder in Seroymx Systems and Leyden Labs. G.A. has served as a scientific advisor for Sanofi Vaccines. G.A. has collaborative agreements with GSK, Merck, Abbvie, Sanofi, Medicago, BioNtech, Moderna, BMS, Novavax, SK Biosciences, Gilead, and Sanaria. R.S.B. has served as a consultant for Takeda and Sanofi Pasteur vaccines and is a member of the Scientific Advisory Board of VaxArt and Invivyd. R.S.B. has unrelated collaborations with J&J, Gilead, Ridgeback Biosciences, and Moderna.

Auteurs

Lily E Adams (LE)

Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Sarah R Leist (SR)

Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Kenneth H Dinnon (KH)

Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Ande West (A)

Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Kendra L Gully (KL)

Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Division of Comparative Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Elizabeth J Anderson (EJ)

Division of Comparative Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Jennifer F Loome (JF)

Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Emily A Madden (EA)

Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

John M Powers (JM)

Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Alexandra Schäfer (A)

Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Sanjay Sarkar (S)

Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Izabella N Castillo (IN)

Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Jenny S Maron (JS)

Ragon Institute of MGH, MIT, and Harvard University, Cambridge, MA, USA.

Ryan P McNamara (RP)

Ragon Institute of MGH, MIT, and Harvard University, Cambridge, MA, USA.

Harry L Bertera (HL)

Ragon Institute of MGH, MIT, and Harvard University, Cambridge, MA, USA.

Mark R Zweigert (MR)

Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Jaclyn S Higgins (JS)

Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Brea K Hampton (BK)

Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Lakshmanane Premkumar (L)

Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Galit Alter (G)

Ragon Institute of MGH, MIT, and Harvard University, Cambridge, MA, USA.

Stephanie A Montgomery (SA)

Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Dallas Tissue Research, Dallas, TX, USA.

Victoria K Baxter (VK)

Division of Comparative Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Mark T Heise (MT)

Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Rapidly Emerging Antiviral Drug Discovery Initiative, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. Electronic address: mark_heisem@med.unc.edu.

Ralph S Baric (RS)

Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Rapidly Emerging Antiviral Drug Discovery Initiative, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. Electronic address: rbaric@email.unc.edu.

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Classifications MeSH