Assessment of cecal microbiota modulation from piglet dietary supplementation with copper.
16S rRNA gene
Animal nutrition
Copper
Feed supplement
Microbiota
Swine
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
31 03 2023
31 03 2023
Historique:
received:
27
12
2021
accepted:
15
03
2023
medline:
4
4
2023
entrez:
2
4
2023
pubmed:
3
4
2023
Statut:
epublish
Résumé
Swine production expanded in the last decades. Efforts have been made to improve meat production and to understand its relationship to pig gut microbiota. Copper (Cu) is a usual supplement to growth performance in animal production. Here, two performance studies were conducted to investigate the effects of three different sources of Cu on the microbiota of piglets. A total of 256 weaned piglets were randomly allocated into 4 treatments (10 replicates per treatment of 4 piglets per pen in Trial 1 and 8 replicates of 3 piglets per pen in Trial 2). Treatments included a control group (fed 10 mg/kg of Cu from CuSO A total of 1337 Operational Taxonomic Units (OTUs) were identified. Dominance and Simpson ecological metrics were statistically different between control and treated groups (P < 0.10) showing that different Cu sources altered the gut microbiota composition with the proliferation of some bacteria that improve gut health. A high abundance of Prevotella was observed in all treatments while other genera were enriched and differentially modulated, according to the Cu source and dosage. The supplementation with Cu-MHAC can modify a group of bacteria involved in feed efficiency (FE) and short chain fatty acids (SCFA) production (Clostridium XIVa, Desulfovibrio, and Megasphera). These bacteria are also important players in the activation of ghrelin and growth hormones that were previously reported to correlate with Cu-MHAC supplementation. These results indicated that some genera seem to be directly affected by the Cu source offered to the animals. TBCC and Cu-MHAC (even in low doses) can promote healthy modifications in the gut bacterial composition, being a promising source of supplementation for piglets.
Sections du résumé
BACKGROUND
Swine production expanded in the last decades. Efforts have been made to improve meat production and to understand its relationship to pig gut microbiota. Copper (Cu) is a usual supplement to growth performance in animal production. Here, two performance studies were conducted to investigate the effects of three different sources of Cu on the microbiota of piglets. A total of 256 weaned piglets were randomly allocated into 4 treatments (10 replicates per treatment of 4 piglets per pen in Trial 1 and 8 replicates of 3 piglets per pen in Trial 2). Treatments included a control group (fed 10 mg/kg of Cu from CuSO
RESULTS
A total of 1337 Operational Taxonomic Units (OTUs) were identified. Dominance and Simpson ecological metrics were statistically different between control and treated groups (P < 0.10) showing that different Cu sources altered the gut microbiota composition with the proliferation of some bacteria that improve gut health. A high abundance of Prevotella was observed in all treatments while other genera were enriched and differentially modulated, according to the Cu source and dosage. The supplementation with Cu-MHAC can modify a group of bacteria involved in feed efficiency (FE) and short chain fatty acids (SCFA) production (Clostridium XIVa, Desulfovibrio, and Megasphera). These bacteria are also important players in the activation of ghrelin and growth hormones that were previously reported to correlate with Cu-MHAC supplementation.
CONCLUSIONS
These results indicated that some genera seem to be directly affected by the Cu source offered to the animals. TBCC and Cu-MHAC (even in low doses) can promote healthy modifications in the gut bacterial composition, being a promising source of supplementation for piglets.
Identifiants
pubmed: 37003969
doi: 10.1186/s12866-023-02826-9
pii: 10.1186/s12866-023-02826-9
pmc: PMC10064724
doi:
Substances chimiques
Copper
789U1901C5
Copper Sulfate
LRX7AJ16DT
Types de publication
Journal Article
Randomized Controlled Trial, Veterinary
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
92Informations de copyright
© 2023. The Author(s).
Références
Nat Rev Gastroenterol Hepatol. 2012 Feb 21;9(4):219-30
pubmed: 22349170
Nature. 2012 Sep 13;489(7415):242-9
pubmed: 22972297
Nature. 2019 Apr;568(7753):499-504
pubmed: 30745586
Front Microbiol. 2013 Jul 30;4:217
pubmed: 23908650
J Hazard Mater. 2021 Jan 5;401:123767
pubmed: 33113734
PLoS One. 2014 Mar 11;9(3):e90731
pubmed: 24618913
Front Microbiol. 2017 Oct 10;8:1967
pubmed: 29067020
Anim Nutr. 2018 Jun;4(2):113-125
pubmed: 30140751
Appl Environ Microbiol. 2017 Aug 17;83(17):
pubmed: 28625984
Dev Comp Immunol. 2022 Jun;131:104375
pubmed: 35219758
Trends Biotechnol. 2015 Sep;33(9):496-503
pubmed: 26210164
Front Vet Sci. 2019 Mar 13;6:73
pubmed: 30918894
Front Microbiol. 2011 May 30;2:93
pubmed: 21747801
J Anim Sci. 1998 Jan;76(1):118-23
pubmed: 9464892
Asian-Australas J Anim Sci. 2014 Jul;27(7):965-73
pubmed: 25050038
Proc Natl Acad Sci U S A. 2016 Mar 1;113(9):E1306-15
pubmed: 26884164
Gut Pathog. 2010 Jul 22;2(1):8
pubmed: 20649986
Anim Nutr. 2022 Mar;8(1):321-330
pubmed: 35024469
Obesity (Silver Spring). 2018 May;26(5):801-809
pubmed: 29687647
Int J Syst Evol Microbiol. 2018 Jun;68(6):1825-1829
pubmed: 29724269
Dig Liver Dis. 2018 Jul;50(7):635-639
pubmed: 29650468
Food Funct. 2020 Jan 29;11(1):472-482
pubmed: 31833510
Sci Rep. 2015 Apr 21;5:9938
pubmed: 25898122
Front Vet Sci. 2018 Feb 13;5:13
pubmed: 29487856
Sci Rep. 2019 Jun 19;9(1):8791
pubmed: 31217427
J Biotechnol. 2015 Jan 20;194:124-31
pubmed: 25500375
Appl Environ Microbiol. 2005 May;71(5):2267-77
pubmed: 15870311
Science. 2012 Jun 8;336(6086):1262-7
pubmed: 22674330
Immunology. 2017 Aug;151(4):363-374
pubmed: 28542929
Sci Rep. 2018 Aug 24;8(1):12727
pubmed: 30143657
Sci Rep. 2021 May 21;11(1):10720
pubmed: 34021225
Science. 2011 Jul 29;333(6042):646-8
pubmed: 21719642
Cell Metab. 2015 Dec 1;22(6):971-82
pubmed: 26552345
PLoS One. 2017 Jan 10;12(1):e0169851
pubmed: 28072880
Bioinformatics. 2010 Oct 1;26(19):2460-1
pubmed: 20709691
Front Microbiol. 2017 Sep 04;8:1688
pubmed: 28928724
ISME J. 2013 May;7(5):949-61
pubmed: 23235287
PLoS One. 2018 Apr 6;13(4):e0195643
pubmed: 29624599
Bioresour Technol. 2015 Dec;197:152-60
pubmed: 26340024
Front Microbiol. 2018 Aug 23;9:2011
pubmed: 30210480
Science. 2011 Oct 7;334(6052):105-8
pubmed: 21885731
BMC Microbiol. 2017 May 22;17(1):120
pubmed: 28532414
Anim Nutr. 2018 Jun;4(2):187-196
pubmed: 30140758
Nucleic Acids Res. 2015 Jul 1;43(W1):W566-70
pubmed: 25969447
Curr Microbiol. 2018 Jun;75(6):745-751
pubmed: 29368025
Vet Microbiol. 2015 Jun 12;177(3-4):242-51
pubmed: 25843944
J Anim Sci Biotechnol. 2020 Feb 20;11:24
pubmed: 32099648
PLoS One. 2018 Aug 1;13(8):e0200322
pubmed: 30067768
Future Microbiol. 2017 Feb;12:157-170
pubmed: 28139139
Microbes Environ. 2015;30(3):284-7
pubmed: 26212519
Front Immunol. 2018 Aug 15;9:1830
pubmed: 30158926
Appl Environ Microbiol. 2014 Jan;80(1):193-203
pubmed: 24141125
Proc Natl Acad Sci U S A. 2010 Aug 17;107(33):14691-6
pubmed: 20679230
mSystems. 2019 May 7;4(3):
pubmed: 31219789
J Water Health. 2010 Mar;8(1):147-57
pubmed: 20009257
Trends Microbiol. 2017 Oct;25(10):851-873
pubmed: 28602521
mBio. 2014 May 06;5(3):e01021-14
pubmed: 24803517
PeerJ. 2019 Aug 16;7:e7502
pubmed: 31440436
Appl Environ Microbiol. 2011 Mar;77(5):1541-7
pubmed: 21193661
Gut Pathog. 2013 Aug 13;5(1):23
pubmed: 23941657
PLoS One. 2013 Nov 18;8(11):e79353
pubmed: 24260205
J Gen Appl Microbiol. 2014;60(4):140-6
pubmed: 25273987
Bioinformatics. 2006 Jun 15;22(12):1540-2
pubmed: 16595560
Proc Natl Acad Sci U S A. 2012 Jan 31;109(5):1691-6
pubmed: 22307632
mSystems. 2017 May 23;2(3):
pubmed: 28567446
Nucleic Acids Res. 2009 Jan;37(Database issue):D141-5
pubmed: 19004872
ISME J. 2014 Aug;8(8):1566-76
pubmed: 24522263
Pol J Vet Sci. 2016 Dec 1;19(4):917-924
pubmed: 28092617
Microbiome. 2019 Jul 30;7(1):109
pubmed: 31362781
Appl Environ Microbiol. 2002 Feb;68(2):673-90
pubmed: 11823207
J Microbiol Immunol Infect. 2017 Jun;50(3):265-269
pubmed: 28655571
J Anim Sci. 2019 Oct 3;97(10):4242-4247
pubmed: 31581297
Lancet Infect Dis. 2018 Nov;18(11):1229-1240
pubmed: 30266330
Anim Health Res Rev. 2012 Jun;13(1):100-9
pubmed: 22853934
Int J Obes (Lond). 2015 Sep;39(9):1331-8
pubmed: 25971927
Clin Microbiol Rev. 2017 Nov 29;31(1):
pubmed: 29187397
Sci Rep. 2018 Apr 16;8(1):6012
pubmed: 29662088
PLoS One. 2013 May 28;8(5):e65465
pubmed: 23724144
Nature. 2016 Jun 08;534(7606):213-7
pubmed: 27279214
PLoS One. 2015 Feb 17;10(2):e0117441
pubmed: 25688558