PGC-1s shape epidermal physiology by modulating keratinocyte proliferation and terminal differentiation.
dermatology
human metabolism
molecular mechanism of gene regulation
physiology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
21 Apr 2023
21 Apr 2023
Historique:
received:
21
07
2022
revised:
02
12
2022
accepted:
26
02
2023
medline:
4
4
2023
entrez:
3
4
2023
pubmed:
4
4
2023
Statut:
epublish
Résumé
Skin plays central roles in systemic physiology, and it undergoes significant functional changes during aging. Members of the peroxisome proliferator-activated receptor-gamma coactivator (PGC-1) family (PGC-1s) are key regulators of the biology of numerous tissues, yet we know very little about their impact on skin functions. Global gene expression profiling and gene silencing in keratinocytes uncovered that PGC-1s control the expression of metabolic genes as well as that of terminal differentiation programs. Glutamine emerged as a key substrate promoting mitochondrial respiration, keratinocyte proliferation, and the expression of PGC-1s and terminal differentiation programs. Importantly, gene silencing of PGC-1s reduced the thickness of a reconstructed living human epidermal equivalent. Exposure of keratinocytes to a salicylic acid derivative potentiated the expression of PGC-1s and terminal differentiation genes and increased mitochondrial respiration. Overall, our results show that the PGC-1s are essential effectors of epidermal physiology, revealing an axis that could be targeted in skin conditions and aging.
Identifiants
pubmed: 37009228
doi: 10.1016/j.isci.2023.106314
pii: S2589-0042(23)00391-7
pmc: PMC10064239
doi:
Types de publication
Journal Article
Langues
eng
Pagination
106314Informations de copyright
© 2023 The Authors.
Déclaration de conflit d'intérêts
The authors have no competing interests to declare.
Références
FEBS J. 2015 Feb;282(4):647-72
pubmed: 25495651
PLoS One. 2010 Nov 15;5(11):e13984
pubmed: 21085593
Methods Mol Biol. 2016;1458:273-90
pubmed: 27581029
Nucleic Acids Res. 2019 Jan 8;47(D1):D330-D338
pubmed: 30395331
Cancer Res. 2017 Sep 15;77(18):5054-5067
pubmed: 28765155
Science. 2012 May 18;336(6083):918-22
pubmed: 22517326
Science. 2014 Oct 3;346(6205):89-93
pubmed: 25147279
Clin Interv Aging. 2016 Feb 12;11:141-8
pubmed: 26929610
Lancet. 2009 Oct 3;374(9696):1196-208
pubmed: 19801098
Cell Metab. 2005 Jun;1(6):361-70
pubmed: 16054085
Cell. 2008 Jul 11;134(1):112-23
pubmed: 18614015
Biochem J. 1965 Oct;97(1):194-8
pubmed: 16749103
Aging (Albany NY). 2018 Jul 18;10(7):1609-1626
pubmed: 30021930
Maturitas. 2011 Jul;69(3):249-56
pubmed: 21612880
Maturitas. 2016 Nov;93:34-40
pubmed: 27215947
Expert Rev Endocrinol Metab. 2012 Jul;7(4):461-472
pubmed: 23144648
Nucleic Acids Res. 2022 Jul 5;50(W1):W216-W221
pubmed: 35325185
Cell Rep. 2016 Sep 13;16(11):2991-3002
pubmed: 27626667
Sci Rep. 2016 Oct 12;6:35065
pubmed: 27731355
Cell. 2013 Jun 6;153(6):1194-217
pubmed: 23746838
Cell. 2011 Mar 4;144(5):646-74
pubmed: 21376230
Genome Biol. 2013 Jul 26;14(7):R75
pubmed: 23889843
Exp Gerontol. 2015 Aug;68:59-65
pubmed: 25262846
Nat Protoc. 2009;4(1):44-57
pubmed: 19131956
J Biol Chem. 2005 Apr 22;280(16):16456-60
pubmed: 15716268
Diabetes. 2016 Nov;65(11):3352-3361
pubmed: 27554471
Biochem Biophys Res Commun. 2014 Sep 26;452(3):622-8
pubmed: 25181340
Diabetologia. 2015 Sep;58(9):1969-77
pubmed: 26109214
J Cachexia Sarcopenia Muscle. 2017 Jun;8(3):349-369
pubmed: 28432755
J Invest Dermatol. 2011 Sep;131(9):1793-805
pubmed: 21633368
Nucleic Acids Res. 2018 Jan 4;46(D1):D1284
pubmed: 29161433
Matrix Biol. 2015 Sep;47:85-97
pubmed: 25840344
IUBMB Life. 2012 Mar;64(3):231-41
pubmed: 22279035
Mech Ageing Dev. 2017 Mar;162:18-26
pubmed: 28163107
Nat Genet. 2000 May;25(1):25-9
pubmed: 10802651
Aging Cell. 2018 Dec;17(6):e12830
pubmed: 30192051
Am J Clin Nutr. 2011 Apr;93(4):884S-90
pubmed: 21289221
Exp Dermatol. 2014 Sep;23(9):607-14
pubmed: 24980550
Exp Dermatol. 2016 Feb;25(2):92-8
pubmed: 26284579
J Cell Mol Med. 2011 Jun;15(6):1239-53
pubmed: 21251211
J Clin Invest. 2004 Nov;114(10):1518-26
pubmed: 15546003
J Investig Dermatol Symp Proc. 2009 Aug;14(1):44-9
pubmed: 19675552
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
Cancer Res. 2004 Jan 15;64(2):472-81
pubmed: 14744759
Aging (Albany NY). 2014 Oct;6(10):856-78
pubmed: 25411231
Am J Physiol Endocrinol Metab. 2014 Jan 15;306(2):E157-67
pubmed: 24280126
Cancer Metab. 2013 Dec 05;1(1):22
pubmed: 24304688
Nat Genet. 2003 Jul;34(3):267-73
pubmed: 12808457
Nature. 2008 Feb 21;451(7181):1008-12
pubmed: 18288196
J Biol Chem. 2003 Sep 5;278(36):34277-85
pubmed: 12810719
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
Gerontologist. 2016 Apr;56 Suppl 2:S230-42
pubmed: 26994263
Nucleic Acids Res. 2009 Jul;37(Web Server issue):W247-52
pubmed: 19487240
Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4721-6
pubmed: 18347329
Methods Mol Biol. 2007;358:177-97
pubmed: 17035687
Bioinformatics. 2009 Apr 1;25(7):875-81
pubmed: 19189975
J Cosmet Dermatol. 2016 Dec;15(4):527-539
pubmed: 27496663
Biopreserv Biobank. 2015 Oct;13(5):311-9
pubmed: 26484571
Cell Metab. 2013 Nov 5;18(5):698-711
pubmed: 24206664
PLoS One. 2017 Jun 23;12(6):e0174469
pubmed: 28644888
Nucleic Acids Res. 2016 Jan 4;44(D1):D1251-7
pubmed: 26450961
J Biol Chem. 2016 Dec 2;291(49):25529-25541
pubmed: 27789709
Microvasc Res. 2015 Jul;100:25-31
pubmed: 25917013
J Biomed Sci. 2004 Sep-Oct;11(5):671-82
pubmed: 15316143
J Physiol. 2016 Apr 15;594(8):2025-42
pubmed: 26293414
FASEB J. 2003 Sep;17(12):1706-8
pubmed: 12958183
Cell. 2000 Jan 7;100(1):57-70
pubmed: 10647931
BMC Genomics. 2017 Feb 15;18(1):169
pubmed: 28201987
Age (Dordr). 2012 Jun;34(3):669-79
pubmed: 21590341
J Invest Dermatol. 2016 May;136(5):912-919
pubmed: 26829036
Biofactors. 2008;32(1-4):245-55
pubmed: 19096122
Skin Res Technol. 1995 Aug;1(3):115-22
pubmed: 27328438
Exp Gerontol. 2014 Aug;56:59-68
pubmed: 24699405
J Cell Sci. 2012 Nov 1;125(Pt 21):4963-71
pubmed: 23277535
Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12017-22
pubmed: 17609368
Gerontology. 2006;52(3):142-53
pubmed: 16645294
Curr Biol. 2012 Sep 11;22(17):R741-52
pubmed: 22975005
J Cosmet Dermatol. 2007 Mar;6(1):59-65
pubmed: 17348998
Aging Cell. 2015 Dec;14(6):1085-93
pubmed: 26365892
Biochim Biophys Acta. 2011 Jul;1813(7):1269-78
pubmed: 20933024
J Biol Chem. 2013 Jan 18;288(3):1685-90
pubmed: 23223238
Sci Signal. 2013 Feb 05;6(261):ra8
pubmed: 23386745
Mol Cell. 2011 Nov 18;44(4):621-34
pubmed: 22099309
Circ Res. 2016 May 13;118(10):1593-611
pubmed: 27174952
Sci Rep. 2017 Jun 19;7(1):3792
pubmed: 28630487