An MltA-Like Lytic Transglycosylase Secreted by Bdellovibrio bacteriovorus Cleaves the Prey Septum during Predatory Invasion.
Bdellovibrio bacteriovorus
MltA
bacterial cell division
bacterial septum
cell wall
lytic transglycosylase
peptidoglycan
peptidoglycan hydrolases
predatory bacteria
Journal
Journal of bacteriology
ISSN: 1098-5530
Titre abrégé: J Bacteriol
Pays: United States
ID NLM: 2985120R
Informations de publication
Date de publication:
25 04 2023
25 04 2023
Historique:
medline:
27
4
2023
pubmed:
4
4
2023
entrez:
3
4
2023
Statut:
ppublish
Résumé
Lytic transglycosylases cut peptidoglycan backbones, facilitating a variety of functions within bacteria, including cell division, pathogenesis, and insertion of macromolecular machinery into the cell envelope. Here, we identify a novel role of a secreted lytic transglycosylase associated with the predatory lifestyle of Bdellovibrio bacteriovorus strain HD100. During wild-type B. bacteriovorus prey invasion, the predator rounds up rod-shaped prey into spherical prey bdelloplasts, forming a spacious niche within which the predator grows. Deleting the MltA-like lytic transglycosylase Bd3285 still permitted predation but resulted in three different, invaded prey cell shapes: spheres, rods, and "dumbbells." Amino acid D321 within the catalytic C-terminal 3D domain of Bd3285 was essential for wild-type complementation. Microscopic analyses revealed that dumbbell-shaped bdelloplasts are derived from Escherichia coli prey undergoing cell division at the moment of Δ
Identifiants
pubmed: 37010281
doi: 10.1128/jb.00475-22
pmc: PMC10127604
doi:
Substances chimiques
Peptidoglycan
0
Amino Acids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0047522Subventions
Organisme : Wellcome Trust (WT)
ID : 215025/Z/18/Z
Organisme : Wellcome Trust (WT)
ID : 209437/Z/17/Z
Références
Mol Microbiol. 2006 Apr;60(2):274-86
pubmed: 16573680
Mol Microbiol. 2003 Jul;49(2):331-45
pubmed: 12828633
Microbiology (Reading). 2021 Jan;167(1):
pubmed: 33465024
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
J Mol Biol. 2005 Oct 7;352(5):1068-80
pubmed: 16139297
ACS Infect Dis. 2017 Sep 8;3(9):624-633
pubmed: 28585815
J Bacteriol. 1966 May;91(5):2006-17
pubmed: 5327913
J Bacteriol. 2010 Dec;192(24):6329-35
pubmed: 20935099
J Biol Chem. 2014 Nov 7;289(45):31029-42
pubmed: 25248745
Appl Environ Microbiol. 2015 Dec 28;82(6):1653-1661
pubmed: 26712556
J Bacteriol. 1972 Sep;111(3):664-73
pubmed: 4559819
Curr Biol. 2016 Dec 19;26(24):3343-3351
pubmed: 27889262
Infect Immun. 2002 Jun;70(6):2752-7
pubmed: 12010959
J Bacteriol. 1968 Oct;96(4):1366-81
pubmed: 4879563
Sci Rep. 2019 Mar 12;9(1):4293
pubmed: 30862785
Nat Commun. 2020 Sep 23;11(1):4817
pubmed: 32968056
Mol Microbiol. 2019 Oct;112(4):1100-1115
pubmed: 31286580
PLoS Pathog. 2012 Feb;8(2):e1002524
pubmed: 22346754
Cell Surf. 2021 May 01;7:100053
pubmed: 34036206
Biochemistry. 1989 Feb 21;28(4):1744-9
pubmed: 2541765
J Bacteriol. 1975 Aug;123(2):481-91
pubmed: 1097411
Antonie Van Leeuwenhoek. 1963;29:217-48
pubmed: 14068454
J Bacteriol. 1985 Sep;163(3):1087-94
pubmed: 4030692
Cell. 2014 Dec 4;159(6):1300-11
pubmed: 25480295
Nat Commun. 2022 Mar 21;13(1):1509
pubmed: 35314810
Genes Dev. 2010 Feb 15;24(4):411-22
pubmed: 20159959
Sci Rep. 2016 May 23;6:26010
pubmed: 27211869
Nat Microbiol. 2016 Jun 20;1(7):16077
pubmed: 27572972
Elife. 2022 Jan 24;11:
pubmed: 35073258
Science. 2004 Jan 30;303(5658):689-92
pubmed: 14752164
FEMS Microbiol Rev. 2008 Mar;32(2):149-67
pubmed: 18194336
Mol Microbiol. 2014 Jul;93(1):113-28
pubmed: 24806796
J Biol Chem. 2007 Jul 20;282(29):21197-205
pubmed: 17502382
J Bacteriol. 1997 Sep;179(17):5465-70
pubmed: 9287002
Crit Rev Biochem Mol Biol. 2017 Oct;52(5):503-542
pubmed: 28644060
Curr Protoc Microbiol. 2008 May;Chapter 7:Unit 7B.2
pubmed: 18770540
J Bacteriol. 2004 Nov;186(22):7811-4
pubmed: 15516597
Infect Immun. 2004 Apr;72(4):1914-9
pubmed: 15039310
Mol Microbiol. 2016 Dec;102(5):865-881
pubmed: 27608412
FEMS Microbiol Lett. 2013 Mar;340(2):109-16
pubmed: 23297829
J Bacteriol. 2007 Nov;189(22):7998-8004
pubmed: 17873041
Nat Microbiol. 2017 Dec;2(12):1648-1657
pubmed: 28974693
Angew Chem Int Ed Engl. 2012 Dec 7;51(50):12519-23
pubmed: 23055266
Nat Commun. 2019 Dec 5;10(1):5567
pubmed: 31804467
FEMS Microbiol Rev. 2008 Mar;32(2):259-86
pubmed: 18266855
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
Proc Natl Acad Sci U S A. 2015 Sep 8;112(36):11347-52
pubmed: 26305949