High-Throughput Venomics.
RP-HPLC
fractionation
high-throughput
high-throughput proteomics
mass spectrometry
proteomics
venomics
venoms
Journal
Journal of proteome research
ISSN: 1535-3907
Titre abrégé: J Proteome Res
Pays: United States
ID NLM: 101128775
Informations de publication
Date de publication:
02 06 2023
02 06 2023
Historique:
medline:
5
6
2023
pubmed:
4
4
2023
entrez:
3
4
2023
Statut:
ppublish
Résumé
In this study, we present high-throughput (HT) venomics, a novel analytical strategy capable of performing a full proteomic analysis of a snake venom within 3 days. This methodology comprises a combination of RP-HPLC-nanofractionation analytics, mass spectrometry analysis, automated in-solution tryptic digestion, and high-throughput proteomics. In-house written scripts were developed to process all the obtained proteomics data by first compiling all Mascot search results for a single venom into a single Excel sheet. Then, a second script plots each of the identified toxins in so-called Protein Score Chromatograms (PSCs). For this, for each toxin, identified protein scores are plotted on the
Identifiants
pubmed: 37010854
doi: 10.1021/acs.jproteome.2c00780
pmc: PMC10243144
doi:
Substances chimiques
Snake Venoms
0
Elapid Venoms
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1734-1746Subventions
Organisme : Medical Research Council
ID : MR/S00016X/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 221712/Z/20/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 221710/Z/20/Z
Pays : United Kingdom
Références
Toxins (Basel). 2019 Feb 06;11(2):
pubmed: 30736335
J Proteomics. 2017 Jul 5;164:33-42
pubmed: 28579478
Proteomics. 2004 Feb;4(2):327-38
pubmed: 14760702
Mol Biosyst. 2016 Nov 15;12(12):3530-3543
pubmed: 27787525
Toxins (Basel). 2016 Nov 24;8(12):
pubmed: 27886134
Br J Haematol. 2017 Jun;177(6):947-959
pubmed: 28233897
Toxicon. 2021 Mar;192:15-31
pubmed: 33417947
FEBS Lett. 2009 Jun 5;583(11):1736-43
pubmed: 19303875
Toxins (Basel). 2017 Sep 18;9(9):
pubmed: 28927001
Toxins (Basel). 2017 May 10;9(5):
pubmed: 28489039
J Mass Spectrom. 2007 Nov;42(11):1405-14
pubmed: 17621391
Biochem J. 2017 Feb 20;474(5):611-634
pubmed: 28219972
Commun Biol. 2018 Apr 19;1:34
pubmed: 30271920
Expert Rev Proteomics. 2018 Jul;15(7):555-568
pubmed: 30005583
J Proteomics. 2011 Aug 24;74(9):1768-80
pubmed: 21723969
Br J Clin Pharmacol. 2016 Mar;81(3):446-52
pubmed: 26256124
Arch Intern Med. 2001 Sep 10;161(16):2030-6
pubmed: 11525706
Lancet. 2010 Jan 2;375(9708):77-88
pubmed: 20109866
Science. 2021 Jan 22;371(6527):386-390
pubmed: 33479150
Toxicon X. 2021 Jun 12;9-10:100070
pubmed: 34195606
Anal Chem. 2016 Oct 4;88(19):9699-9706
pubmed: 27593495
Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9205-10
pubmed: 24927555
Front Pharmacol. 2022 Jan 14;12:768015
pubmed: 35095489