Palmitoylation regulates neuropilin-2 localization and function in cortical neurons and conveys specificity to semaphorin signaling via palmitoyl acyltransferases.
Sema3F
cortical neurons
mouse
neuropilin-2
neuropilins
neuroscience
palmitoyl acyltransferases
palmitoylation
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
03 04 2023
03 04 2023
Historique:
received:
02
09
2022
accepted:
22
02
2023
medline:
5
4
2023
entrez:
3
4
2023
pubmed:
4
4
2023
Statut:
epublish
Résumé
Secreted semaphorin 3F (Sema3F) and semaphorin 3A (Sema3A) exhibit remarkably distinct effects on deep layer excitatory cortical pyramidal neurons; Sema3F mediates dendritic spine pruning, whereas Sema3A promotes the elaboration of basal dendrites. Sema3F and Sema3A signal through distinct holoreceptors that include neuropilin-2 (Nrp2)/plexinA3 (PlexA3) and neuropilin-1 (Nrp1)/PlexA4, respectively. We find that Nrp2 and Nrp1 are S-palmitoylated in cortical neurons and that palmitoylation of select Nrp2 cysteines is required for its proper subcellular localization, cell surface clustering, and also for Sema3F/Nrp2-dependent dendritic spine pruning in cortical neurons, both in vitro and in vivo. Moreover, we show that the palmitoyl acyltransferase ZDHHC15 is required for Nrp2 palmitoylation and Sema3F/Nrp2-dependent dendritic spine pruning, but it is dispensable for Nrp1 palmitoylation and Sema3A/Nrp1-dependent basal dendritic elaboration. Therefore, palmitoyl acyltransferase-substrate specificity is essential for establishing compartmentalized neuronal structure and functional responses to extrinsic guidance cues.
Identifiants
pubmed: 37010951
doi: 10.7554/eLife.83217
pii: 83217
pmc: PMC10069869
doi:
pii:
Substances chimiques
Semaphorins
0
Semaphorin-3A
0
Neuropilin-2
0
Neuropilin-1
144713-63-3
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Howard Hughes Medical Institute
ID : Previous support
Pays : United States
Organisme : NIH HHS
ID : R21NS085358
Pays : United States
Organisme : NIH HHS
ID : MH100024-Project #3
Pays : United States
Organisme : NIMH NIH HHS
ID : P50 MH100024
Pays : United States
Organisme : NICHD NIH HHS
ID : P50 HD105351
Pays : United States
Organisme : NINDS NIH HHS
ID : R21 NS085358
Pays : United States
Informations de copyright
© 2023, Koropouli et al.
Déclaration de conflit d'intérêts
EK, QW, RM, TW, AK No competing interests declared, RH Randal Hand is affiliated with Prilenia Therapeutics Development LTD. The author has no financial interests to declare, DG Reviewing editor, eLife
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