FGF4 and FGF9 have synergistic effects on odontoblast differentiation.
Dmp1
Enamel knot
FGF4
FGF9
Mesenchymal cells
Odontoblast
Journal
Medical molecular morphology
ISSN: 1860-1499
Titre abrégé: Med Mol Morphol
Pays: Japan
ID NLM: 101239023
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
28
11
2022
accepted:
06
03
2023
medline:
23
10
2023
pubmed:
4
4
2023
entrez:
3
4
2023
Statut:
ppublish
Résumé
The purpose of this study was to investigate whether fibroblast growth factor 4 (FGF4) and FGF9 are active in dentin differentiation. Dentin matrix protein 1 (Dmp1) -2A-Cre transgenic mice, which express the Cre-recombinase in Dmp1-expressing cells, were crossed with CAG-tdTomato mice as reporter mouse. The cell proliferation and tdTomato expressions were observed. The mesenchymal cell separated from neonatal molar tooth germ were cultured with or without FGF4, FGF9, and with or without their inhibitors ferulic acid and infigratinib (BGJ398) for 21 days. Their phenotypes were evaluated by cell count, flow cytometry, and real-time PCR. Immunohistochemistry for FGFR1, 2, and 3 expression and the expression of DMP1 were performed. FGF4 treatment of mesenchymal cells obtained promoted the expression of all odontoblast markers. FGF9 failed to enhance dentin sialophosphoprotein (Dspp) expression levels. Runt-related transcription factor 2 (Runx2) was upregulated until day 14 but was downregulated on day 21. Compared to Dmp1-negative cells, Dmp1-positive cells expressed higher levels of all odontoblast markers, except for Runx2. Simultaneous treatment with FGF4 and FGF9 had a synergistic effect on odontoblast differentiation, suggesting that they may play a role in odontoblast maturation.
Identifiants
pubmed: 37012505
doi: 10.1007/s00795-023-00351-2
pii: 10.1007/s00795-023-00351-2
doi:
Substances chimiques
Core Binding Factor Alpha 1 Subunit
0
Fibroblast Growth Factor 4
0
Fgf4 protein, mouse
0
Fgf9 protein, mouse
0
Fibroblast Growth Factor 9
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
159-176Subventions
Organisme : Japan Society for the Promotion of Science
ID : 18H03007
Organisme : Japan Society for the Promotion of Science
ID : 21H03146
Organisme : Japan Society for the Promotion of Science
ID : 19K19074
Organisme : Exploratory Research for Advanced Technology
ID : Suematsu Gas Biology
Informations de copyright
© 2023. The Author(s) under exclusive licence to The Japanese Society for Clinical Molecular Morphology.
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