Cloning and Functional Characterization of 2-C-methyl-D-erythritol-4-phosphate cytidylyltransferase (LiMCT) Gene in Oriental Lily (Lilium 'Sorbonne').

Floral scent LiMCT Lilium Methylerythritol pathway Monoterpenes biosynthesis

Journal

Molecular biotechnology
ISSN: 1559-0305
Titre abrégé: Mol Biotechnol
Pays: Switzerland
ID NLM: 9423533

Informations de publication

Date de publication:
04 Apr 2023
Historique:
received: 04 02 2023
accepted: 21 03 2023
entrez: 4 4 2023
pubmed: 5 4 2023
medline: 5 4 2023
Statut: aheadofprint

Résumé

2-C-methyl-D-erythritol-phosphate cytidylyltransferase (MCT) is a key enzyme in the MEP pathway of monoterpene synthesis, catalyzing the generation of 4- (5'-pyrophosphate cytidine)-2-C-methyl-D-erythritol from 2-C-methyl-D-erythritol-4-phosphate. We used homologous cloning strategy to clone gene, LiMCT, in the MEP pathway that may be involved in the regulation of floral fragrance synthesis in the Lilium oriental hybrid 'Sorbonne.' The full-length ORF sequence was 837 bp, encoding 278 amino acids. Bioinformatics analysis showed that the relative molecular weight of LiMCT protein is 68.56 kD and the isoelectric point (pI) is 5.12. The expression pattern of LiMCT gene was found to be consistent with the accumulation sites and emission patterns of floral fragrance monoterpenes in transcriptome data (unpublished). Subcellular localization indicated that the LiMCT protein is located in chloroplasts, which is consistent with the location of MEP pathway genes functioning in plastids to produce isoprene precursors. Overexpression of LiMCT in Arabidopsis thaliana affected the expression levels of MEP and MVA pathway genes, suggesting that overexpression of the LiMCT in A. thaliana affected the metabolic flow of C5 precursors of two different terpene synthesis pathways. The expression of the monoterpene synthase AtTPS14 was elevated nearly fourfold in transgenic A. thaliana compared with the control, and the levels of carotenoids and chlorophylls, the end products of the MEP pathway, were significantly increased in the leaves at full bloom, indicating that LiMCT plays an important role in regulating monoterpene synthesis and in the synthesis of other isoprene-like precursors in transgenic A. thaliana flowers. However, the specific mechanism of LiMCT in promoting the accumulation of isoprene products of the MEP pathway and the biosynthesis of floral monoterpene volatile components needs further investigation.

Identifiants

pubmed: 37014586
doi: 10.1007/s12033-023-00729-8
pii: 10.1007/s12033-023-00729-8
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Fan Jiang (F)

College of Horticulture and Landscape, Northeast Agricultural University, Harbin, 150030, China.

Dongying Liu (D)

College of Horticulture and Landscape, Northeast Agricultural University, Harbin, 150030, China.

Jingqi Dai (J)

College of Horticulture and Landscape, Northeast Agricultural University, Harbin, 150030, China.

Tao Yang (T)

College of Horticulture and Landscape, Northeast Agricultural University, Harbin, 150030, China.

Jinzhu Zhang (J)

College of Horticulture and Landscape, Northeast Agricultural University, Harbin, 150030, China.

Daidi Che (D)

College of Horticulture and Landscape, Northeast Agricultural University, Harbin, 150030, China.

Jinping Fan (J)

College of Horticulture and Landscape, Northeast Agricultural University, Harbin, 150030, China. jinpingfan@neau.edu.cn.

Classifications MeSH