Biseugenol from Ocotea cymbarum (Lauraceae) attenuates inflammation, angiogenesis and collagen deposition of sponge-induced fibrovascular tissue in mice.
Anti-angiogenic
Anti-inflammatory
Chronic inflammation
Natural product
Journal
Inflammopharmacology
ISSN: 1568-5608
Titre abrégé: Inflammopharmacology
Pays: Switzerland
ID NLM: 9112626
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
received:
26
07
2022
accepted:
21
03
2023
medline:
1
6
2023
pubmed:
7
4
2023
entrez:
6
4
2023
Statut:
ppublish
Résumé
Several species of the genus Ocotea are used in traditional medicine due to their anti-inflammatory and analgesic properties. In this work we sought to investigate the effects of biseugenol, the main component of the hexane extract from the leaves of Ocotea cymbarum (Lauraceae), during a chronic inflammatory process induced by polyester-polyurethane sponge in mice. In addition to the inflammatory component, sponge discs also allowed us to evaluate parameters associated with the formation of new blood vessels and the deposition and organization of the extracellular matrix, processes that are related to the chronification of the inflammatory response. Daily treatment with biseugenol (0.1, 1 or 10 µg in 10 µl of 0.5% DMSO) inhibited the synthesis of inflammatory cytokines (TNF-α, CXCL-1 and CCL2) and the neutrophil and macrophage infiltrate into to the implants, indirectly evaluated by the activity of myeloperoxidase and N-acetyl-β-D-glycosaminidase enzymes, respectively. In implants treated with biseugenol, we observed a reduction in angiogenesis, assessed through histological quantification of mean number of blood vessels, the levels of the pro-angiogenic cytokines FGF and VEGF and the activity of metalloproteinases. Except for VEGF levels, all mentioned parameters showed significant reductions after treatment with biseugenol. Finally, the administration of the compound also reduced TGF-β1 levels, collagen synthesis and deposition, in addition to modifying the organization of the newly formed matrix, presenting a potential anti-fibrotic effect. Therefore, our results demonstrate the potential therapeutic use of biseugenol for the treatment of a series of pathological conditions, where parameters associated with inflammation, angiogenesis and fibrogenesis are deregulated.
Identifiants
pubmed: 37022573
doi: 10.1007/s10787-023-01210-3
pii: 10.1007/s10787-023-01210-3
doi:
Substances chimiques
Vascular Endothelial Growth Factor A
0
Collagen
9007-34-5
Cytokines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1539-1549Subventions
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 446480/2014-2
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 301354-2019-7
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 150814/2021-6
Organisme : Fundação de Amparo à Pesquisa do Estado de Minas Gerais
ID : APQ-04436-10
Organisme : Fundação de Amparo à Pesquisa do Estado de Minas Gerais
ID : APQ-02238-14
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2021/02789-7
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.
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