Application of TraDIS to define the core essential genome of Campylobacter jejuni and Campylobacter coli.
Campylobacter coli
Campylobacter jejuni
Campylobacter spp
Essential genes
TraDIS
Transposon mutagenesis
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
06 04 2023
06 04 2023
Historique:
received:
11
08
2022
accepted:
23
03
2023
medline:
10
4
2023
entrez:
6
4
2023
pubmed:
7
4
2023
Statut:
epublish
Résumé
Campylobacter species are the major cause of bacterial gastroenteritis. As there is no effective vaccine, combined with the rapid increase in antimicrobial resistant strains, there is a need to identify new targets for intervention. Essential genes are those that are necessary for growth and/or survival, making these attractive targets. In this study, comprehensive transposon mutant libraries were created in six C. jejuni strains, four C. coli strains and one C. lari and C. hyointestinalis strain, allowing for those genes that cannot tolerate a transposon insertion being called as essential. Comparison of essential gene lists using core genome analysis can highlight those genes which are common across multiple strains and/or species. Comparison of C. jejuni and C. coli, the two species that cause the most disease, identified 316 essential genes. Genes of interest highlighted members of the purine pathway being essential for C. jejuni whilst also finding that a functional potassium uptake system is essential. Protein-protein interaction networks using these essential gene lists also highlighted proteins in the purine pathway being major 'hub' proteins which have a large number of interactors across the network. When adding in two more species (C. lari and C. hyointestinalis) the essential gene list reduces to 261. Within these 261 essential genes, there are many genes that have been found to be essential in other bacteria. These include htrB and PEB4, which have previously been found as core virulence genes across Campylobacter species in other studies. There were 21 genes which have no known function with eight of these being associated with the membrane. These surface-associated essential genes may provide attractive targets. The essential gene lists presented will help to prioritise targets for the development of novel therapeutic and preventative interventions.
Identifiants
pubmed: 37024800
doi: 10.1186/s12866-023-02835-8
pii: 10.1186/s12866-023-02835-8
pmc: PMC10077673
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
97Informations de copyright
© 2023. The Author(s).
Références
BMC Genomics. 2006 Jul 04;7:167
pubmed: 16817973
BMC Genomics. 2017 Aug 14;18(1):616
pubmed: 28806924
J Clin Microbiol. 2002 Jul;40(7):2601-5
pubmed: 12089284
Proteins. 2023 Mar;91(3):293-299
pubmed: 36201627
Clin Vaccine Immunol. 2012 Feb;19(2):113-9
pubmed: 22155767
Bioinformatics. 2004 Feb 12;20(3):340-8
pubmed: 14960460
mSystems. 2019 Jun 25;4(4):
pubmed: 31239393
Science. 2008 Apr 11;320(5873):237-9
pubmed: 18403712
Res Microbiol. 2007 Oct-Nov;158(8-9):644-50
pubmed: 17890061
FEBS J. 2015 Sep;282(17):3395-3411
pubmed: 26084810
Iran J Pharm Res. 2020 Fall;19(4):121-134
pubmed: 33841528
BMC Syst Biol. 2014;8 Suppl 4:S11
pubmed: 25521941
Microbiol Spectr. 2022 Oct 26;10(5):e0102922
pubmed: 36069574
Nature. 2000 Feb 10;403(6770):665-8
pubmed: 10688204
PLoS Biol. 2017 May 19;15(5):e2001390
pubmed: 28542173
Bioinformatics. 2016 Apr 1;32(7):1109-11
pubmed: 26794317
Mol Omics. 2020 Aug 10;16(4):287-304
pubmed: 32347268
Int J Environ Res Public Health. 2013 Nov 26;10(12):6292-304
pubmed: 24287853
J Bacteriol. 2007 Apr;189(7):2844-53
pubmed: 17277072
Psychometrika. 1966 Dec;31(4):581-603
pubmed: 5232444
Mol Biol Evol. 2021 Dec 9;38(12):5825-5829
pubmed: 34597405
Mol Ecol. 2013 Feb;22(4):1051-64
pubmed: 23279096
Appl Environ Microbiol. 2006 Aug;72(8):5666-9
pubmed: 16885327
Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):16043-8
pubmed: 16230626
Front Microbiol. 2017 Mar 28;8:531
pubmed: 28400767
Microbiology (Reading). 2010 Apr;156(Pt 4):1134-1143
pubmed: 20035004
J Med Microbiol. 2006 Jun;55(Pt 6):741-746
pubmed: 16687593
Microb Genom. 2021 Nov;7(11):
pubmed: 34739369
PLoS One. 2016 Feb 05;11(2):e0148222
pubmed: 26848972
PLoS Pathog. 2020 Mar 9;16(3):e1008344
pubmed: 32150575
Trends Microbiol. 2022 Aug;30(8):793-804
pubmed: 35074276
PLoS Genet. 2006 Jun 2;2(6):e88
pubmed: 16751849
Microbiology (Reading). 2015 Dec;161(12):2316-27
pubmed: 26385289
Genome Res. 2009 Dec;19(12):2308-16
pubmed: 19826075
Microbiology (Reading). 2014 Sep;160(Pt 9):2053-2066
pubmed: 24961951
Genome Announc. 2013 Dec 12;1(6):
pubmed: 24336384
J Bacteriol. 2008 Feb;190(3):915-25
pubmed: 18065531
Microbiology (Reading). 2007 Jan;153(Pt 1):254-62
pubmed: 17185554
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
Nat Rev Gastroenterol Hepatol. 2011 Oct 25;8(12):669-85
pubmed: 22025030
Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11428-33
pubmed: 10500193
Nucleic Acids Res. 2021 Jan 8;49(D1):D545-D551
pubmed: 33125081
Mol Microbiol. 2006 Sep;61(6):1646-59
pubmed: 16899076
Microb Pathog. 2021 Sep;158:105059
pubmed: 34157412
BMC Genomics. 2011 Nov 01;12:535
pubmed: 22044676
J Bacteriol. 2021 Mar 8;203(7):
pubmed: 33257523
Appl Environ Microbiol. 2012 Mar;78(5):1411-5
pubmed: 22194296
Sci Rep. 2017 Apr 28;7(1):1251
pubmed: 28455506
mBio. 2018 Feb 20;9(1):
pubmed: 29463657
Appl Environ Microbiol. 2017 Mar 17;83(7):
pubmed: 28115376
Appl Environ Microbiol. 2005 Dec;71(12):8031-41
pubmed: 16332783
Curr Opin Infect Dis. 2014 Oct;27(5):444-50
pubmed: 25023741
Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613
pubmed: 30476243
BMC Microbiol. 2009 Aug 08;9:160
pubmed: 19664234
Biochem Pharmacol. 2017 Jun 15;134:74-86
pubmed: 27940263
FEMS Microbiol Lett. 2009 Nov;300(2):188-94
pubmed: 19824902
J Microbiol Methods. 2013 Oct;95(1):39-47
pubmed: 23810971
Front Plant Sci. 2018 Jun 05;9:694
pubmed: 29922309
J Clin Microbiol. 1985 May;21(5):715-20
pubmed: 3998100
Nat Rev Genet. 2020 Sep;21(9):526-540
pubmed: 32533119
Nucleic Acids Res. 2000 Jan 1;28(1):33-6
pubmed: 10592175
mBio. 2015 Sep 29;6(5):e00612-15
pubmed: 26419875
ISME J. 2016 Mar;10(3):721-9
pubmed: 26305157
Clin Infect Dis. 2009 Apr 15;48(8):1072-8
pubmed: 19275496
Front Microbiol. 2021 Jun 24;12:665858
pubmed: 34248875
Nucleic Acids Res. 2023 Jan 6;51(D1):D418-D427
pubmed: 36350672
PLoS One. 2010 Aug 26;5(8):e12253
pubmed: 20865039
Biochim Biophys Acta. 2016 May;1858(5):980-7
pubmed: 26514603
Nature. 2001 May 3;411(6833):41-2
pubmed: 11333967
Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):966-71
pubmed: 11805338
Clin Vaccine Immunol. 2010 Nov;17(11):1666-71
pubmed: 20861330
Genome Biol. 2020 Jul 22;21(1):180
pubmed: 32698896
Nucleic Acids Res. 2000 Jan 1;28(1):27-30
pubmed: 10592173
Clin Microbiol Rev. 2015 Jul;28(3):687-720
pubmed: 26062576
FEMS Microbiol Lett. 1997 May 1;150(1):141-7
pubmed: 9163918
Infect Immun. 2006 Aug;74(8):4694-707
pubmed: 16861657
J Clin Microbiol. 1987 Apr;25(4):685-91
pubmed: 3571477
Wellcome Open Res. 2018 Sep 24;3:124
pubmed: 30345391
BMC Bioinformatics. 2009 Dec 15;10:421
pubmed: 20003500
PLoS One. 2019 Jan 11;14(1):e0210351
pubmed: 30633767
Cell. 2016 Jun 2;165(6):1493-1506
pubmed: 27238023
Nucleic Acids Res. 2019 Jan 8;47(D1):D309-D314
pubmed: 30418610
J Infect Dis. 1988 Mar;157(3):472-9
pubmed: 3343522