The role of vitamin D receptor and IL-6 in COVID-19.
SARS-CoV-2
cytokine
gene expression
interleukin-6
vitamin D receptor
Journal
Molecular genetics & genomic medicine
ISSN: 2324-9269
Titre abrégé: Mol Genet Genomic Med
Pays: United States
ID NLM: 101603758
Informations de publication
Date de publication:
07 2023
07 2023
Historique:
revised:
26
02
2023
received:
14
07
2022
accepted:
14
03
2023
medline:
13
7
2023
pubmed:
8
4
2023
entrez:
7
4
2023
Statut:
ppublish
Résumé
Vitamin D (Vit.D) has an important role in protecting COVID-19 patients. This study investigated the changes in vitamin D receptor (VDR) expression and interleukin 6 levels in patients with COVID-19. 120 hospitalized patients and 120 healthy people participated in this study, both group adjusted by sex and age. Vit.D was measured with HPLC, the expression of VDR gene was done with Real-time PCR, and IL-6 was measured with ELISA assay. Our findings showed no significant difference in the case of Vit.D (25-OH-D3) between the two studied groups, interestingly the expression of VDR was statistically lower in the patients with COVID-19, p-value = 0.003. VDR expression was lower in the patient with diabetes, hypertension and cardiovascular disease, significantly, p-value = 0.002. The level of IL-6 was statistically higher in the COVID-19 group, p-value = 0.003. Alongside the important role of 25-OH-D3 in COVID-19 patients, the quality and quantity of the VDR expression and its role in the level of IL-6 are the promising risk factors in the future. Further studies are needed to determine the factors increasing the expression level of VDR, especially in the patients with diabetes, hypertension and cardiovascular disease.
Sections du résumé
BACKGROUND
Vitamin D (Vit.D) has an important role in protecting COVID-19 patients. This study investigated the changes in vitamin D receptor (VDR) expression and interleukin 6 levels in patients with COVID-19.
MATERIALS AND METHODS
120 hospitalized patients and 120 healthy people participated in this study, both group adjusted by sex and age. Vit.D was measured with HPLC, the expression of VDR gene was done with Real-time PCR, and IL-6 was measured with ELISA assay.
RESULTS
Our findings showed no significant difference in the case of Vit.D (25-OH-D3) between the two studied groups, interestingly the expression of VDR was statistically lower in the patients with COVID-19, p-value = 0.003. VDR expression was lower in the patient with diabetes, hypertension and cardiovascular disease, significantly, p-value = 0.002. The level of IL-6 was statistically higher in the COVID-19 group, p-value = 0.003.
CONCLUSION
Alongside the important role of 25-OH-D3 in COVID-19 patients, the quality and quantity of the VDR expression and its role in the level of IL-6 are the promising risk factors in the future. Further studies are needed to determine the factors increasing the expression level of VDR, especially in the patients with diabetes, hypertension and cardiovascular disease.
Identifiants
pubmed: 37025056
doi: 10.1002/mgg3.2172
pmc: PMC10337279
doi:
Substances chimiques
Interleukin-6
0
Receptors, Calcitriol
0
Vitamin D
1406-16-2
Vitamins
0
VDR protein, human
0
IL6 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2172Informations de copyright
© 2023 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals LLC.
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