Zebrafish dnm1a gene plays a role in the formation of axons and synapses in the nervous tissue.
DAB (RRID:AB_2335241)
Fiji software (RRID:SCR_002285)
Prism GraphPad Software (RRID:SCR_002798)
classical dynamins
developmental and epileptic encephalopathy (DEE) disease
dnm1a
mouse anti-acetylated tubulin (RRID:AB_609894)
nervous tissue
neurodevelopment
pBluescript SK- (RRID:Addgene_12306)
vesicular transport protein
zebrafish AB strain (RRID:ZIRC_ZL1)
zebrafish embryos
Journal
Journal of neuroscience research
ISSN: 1097-4547
Titre abrégé: J Neurosci Res
Pays: United States
ID NLM: 7600111
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
revised:
27
02
2023
received:
29
11
2022
accepted:
24
03
2023
medline:
23
6
2023
pubmed:
10
4
2023
entrez:
9
4
2023
Statut:
ppublish
Résumé
Classical dynamins (DNMs) are GTPase proteins engaged in endocytosis, a fundamental process for cargo internalization from the plasma membrane. In mammals, three DNM genes are present with different expression patterns. DNM1 is expressed at high levels in neurons, where it takes place in the recycling of synaptic vesicles; DNM2 is ubiquitously expressed, while DNM3 is found in the brain and in the testis. Due to the conservation of genes in comparison to mammals, we took advantage of a zebrafish model for functional characterization of dnm1a, ortholog of mammalian DNM1. Our data strongly demonstrated that dnm1a has a nervous tissue-specific expression pattern and plays a role in the formation of both axon and synapse. This is the first in vivo study that collects evidence about the effects of dnm1a loss of function in zebrafish, thus providing a new excellent model to be used in different scientific fields.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1345-1359Informations de copyright
© 2023 The Authors. Journal of Neuroscience Research published by Wiley Periodicals LLC.
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