A simple goniometer-compatible flow cell for serial synchrotron X-ray crystallography.
cytochrome c oxidase
goniometer-compatible flow cells
macromolecular crystallography
serial synchrotron X-ray crystallography
Journal
Journal of applied crystallography
ISSN: 0021-8898
Titre abrégé: J Appl Crystallogr
Pays: United States
ID NLM: 9876190
Informations de publication
Date de publication:
01 Apr 2023
01 Apr 2023
Historique:
received:
05
07
2022
accepted:
03
02
2023
medline:
11
4
2023
entrez:
10
4
2023
pubmed:
11
4
2023
Statut:
epublish
Résumé
Serial femtosecond crystallography was initially developed for room-temperature X-ray diffraction studies of macromolecules at X-ray free electron lasers. When combined with tools that initiate biological reactions within microcrystals, time-resolved serial crystallography allows the study of structural changes that occur during an enzyme catalytic reaction. Serial synchrotron X-ray crystallography (SSX), which extends serial crystallography methods to synchrotron radiation sources, is expanding the scientific community using serial diffraction methods. This report presents a simple flow cell that can be used to deliver microcrystals across an X-ray beam during SSX studies. This device consists of an X-ray transparent glass capillary mounted on a goniometer-compatible 3D-printed support and is connected to a syringe pump via light-weight tubing. This flow cell is easily mounted and aligned, and it is disposable so can be rapidly replaced when blocked. This system was demonstrated by collecting SSX data at MAX IV Laboratory from microcrystals of the integral membrane protein cytochrome
Identifiants
pubmed: 37032973
doi: 10.1107/S1600576723001036
pii: S1600576723001036
pmc: PMC10077854
doi:
Types de publication
Journal Article
Langues
eng
Pagination
449-460Informations de copyright
© Swagatha Ghosh et al. 2023.
Références
Proc Natl Acad Sci U S A. 2020 Feb 25;117(8):4142-4151
pubmed: 32047034
Nat Commun. 2014;5:3309
pubmed: 24525480
PLoS One. 2011;6(7):e22348
pubmed: 21814577
IUCrJ. 2020 Jan 16;7(Pt 2):207-219
pubmed: 32148849
Acta Crystallogr D Biol Crystallogr. 2001 Jan;57(Pt 1):122-33
pubmed: 11134934
Acta Crystallogr D Biol Crystallogr. 2015 Oct;71(Pt 10):1987-97
pubmed: 26457423
Curr Opin Struct Biol. 2020 Dec;65:168-174
pubmed: 32846363
Acta Crystallogr D Struct Biol. 2021 Jun 1;77(Pt 6):820-834
pubmed: 34076595
Science. 2021 Aug 27;373(6558):
pubmed: 34446579
Nature. 2015 Sep 3;525(7567):15-6
pubmed: 26333449
Nat Commun. 2017 Nov 3;8(1):1281
pubmed: 29097720
Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25634-25640
pubmed: 31801874
Acta Crystallogr D Struct Biol. 2016 Aug;72(Pt 8):944-55
pubmed: 27487825
Nat Methods. 2017 Apr;14(4):443-449
pubmed: 28250468
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42
pubmed: 21460441
Nat Methods. 2019 Oct;16(10):979-982
pubmed: 31527838
Science. 2014 Dec 5;346(6214):1242-6
pubmed: 25477465
Protein Sci. 2018 Jan;27(1):217-228
pubmed: 28921782
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67
pubmed: 21460454
Rev Sci Instrum. 2012 Mar;83(3):035108
pubmed: 22462961
Philos Trans R Soc Lond B Biol Sci. 2014 Jul 17;369(1647):20130313
pubmed: 24914146
IUCrJ. 2019 Jun 19;6(Pt 4):714-728
pubmed: 31316815
Acta Crystallogr D Struct Biol. 2022 Sep 1;78(Pt 9):1079-1089
pubmed: 36048148
Protein Expr Purif. 2005 Apr;40(2):299-318
pubmed: 15766872
BMC Biol. 2018 May 31;16(1):59
pubmed: 29848358
Anal Chem. 2019 Jun 4;91(11):7139-7144
pubmed: 31060352
J Appl Crystallogr. 2016 Mar 29;49(Pt 2):680-689
pubmed: 27047311
Nat Protoc. 2018 Feb;13(2):260-292
pubmed: 29300389
J Synchrotron Radiat. 2014 Nov;21(Pt 6):1364-6
pubmed: 25343806
Struct Dyn. 2015 Aug 18;2(5):054302
pubmed: 26798825
J Synchrotron Radiat. 2020 Sep 1;27(Pt 5):1415-1429
pubmed: 32876619
IUCrJ. 2014 Feb 10;1(Pt 2):87-94
pubmed: 25075324
Biochemistry. 2009 Feb 10;48(5):820-6
pubmed: 19140675
IUCrJ. 2014 May 30;1(Pt 4):204-12
pubmed: 25075341
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):861-877
pubmed: 31588918
J Synchrotron Radiat. 2022 Mar 1;29(Pt 2):331-346
pubmed: 35254295
J Biol Chem. 2020 Sep 25;295(39):13488-13501
pubmed: 32723869
Science. 2019 Oct 18;366(6463):334-338
pubmed: 31624207
Sci Rep. 2017 Jul 3;7(1):4518
pubmed: 28674417
Inorg Chem. 2020 Jul 6;59(13):8906-8915
pubmed: 32525689
Nat Methods. 2018 Nov;15(11):901-904
pubmed: 30377366
J Appl Crystallogr. 2019 Oct 17;52(Pt 6):1280-1288
pubmed: 31798359
Science. 2019 Sep 13;365(6458):1167-1170
pubmed: 31515393
J Synchrotron Radiat. 2015 Nov;22(6):1372-8
pubmed: 26524301
Struct Dyn. 2016 Aug 29;3(5):054301
pubmed: 27679802
IUCrJ. 2015 Feb 03;2(Pt 2):246-55
pubmed: 25866661
Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16169-73
pubmed: 19805275
Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):937-946
pubmed: 31588925
Nat Commun. 2017 Sep 14;8(1):542
pubmed: 28912485
J Synchrotron Radiat. 2020 Mar 1;27(Pt 2):360-370
pubmed: 32153274
IUCrJ. 2019 Jun 12;6(Pt 4):665-680
pubmed: 31316810
Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3397-402
pubmed: 25733886
Nature. 2000 Aug 17;406(6797):752-7
pubmed: 10963603
Science. 2019 Jul 5;365(6448):61-65
pubmed: 31273117
EMBO J. 2000 Apr 17;19(8):1766-76
pubmed: 10775261
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
Biochim Biophys Acta. 2007 Dec;1767(12):1383-92
pubmed: 17964277
IUCrJ. 2015 Jan 27;2(Pt 2):168-76
pubmed: 25866654
Biochim Biophys Acta. 1994 Jan 4;1183(3):504-12
pubmed: 8286399
Nature. 2017 Jan 12;541(7636):242-246
pubmed: 27841871
IUCrJ. 2017 Oct 09;4(Pt 6):769-777
pubmed: 29123679
J Synchrotron Radiat. 2009 Mar;16(Pt 2):152-62
pubmed: 19240327
Methods Mol Biol. 2017;1607:239-272
pubmed: 28573576
Acta Crystallogr D Biol Crystallogr. 2015 Feb;71(Pt 2):387-97
pubmed: 25664750
Nat Methods. 2015 Jan;12(1):61-3
pubmed: 25384243