β-defensin-4 as an endogenous biomarker in cows with mastitis.
antimicrobial peptide
biomarker
dairy cow
defensin
mastitis
Journal
Frontiers in veterinary science
ISSN: 2297-1769
Titre abrégé: Front Vet Sci
Pays: Switzerland
ID NLM: 101666658
Informations de publication
Date de publication:
2023
2023
Historique:
received:
30
01
2023
accepted:
09
03
2023
medline:
11
4
2023
entrez:
10
4
2023
pubmed:
11
4
2023
Statut:
epublish
Résumé
Defensins are peptides with antimicrobial and immunomodulatory effects. Their concentration could be altered during infections and thus provide information on the prognosis and course of the disease. The aim of the present study was to investigate the defensin concentration in cows with mastitis in order to find correlations between clinical expression and course of the disease and the defensin concentration in milk and blood. A total of 85 dairy cows were examined. Of these, 30 animals suffered from acute clinical mastitis, 25 animals were diagnosed with subclinical mastitis and 30 animals were considered a healthy comparison group. Beta-Defensin-4 (DEFB-4) was determined by a species-specific enzyme-linked immunosorbent assay (ELISA) (Bovine Defensin Beta 4 ELISA Kit, MyBioSource). The highest concentrations of DEFB-4 were detected in the animals with acute clinical mastitis. Values of 0 to 895 pg/mL (median: 115 pg/mL) were measured in milk and 40-1,016 pg/mL (median: 245 pg/mL) in serum. The concentrations of this group differed significantly from those of the animals with subclinical mastitis ( Our results also show that in cases of acute mastitis after 12 days of treatment there is still an active inflammatory process in the tissue, because no significant reduction of somatic cells and defensin could be found after re-examination. Since the DEFB-4 concentrations of animals with clinical mastitis that had to be treated with antibiotics differed significantly from those of animals with subclinical mastitis that did not require antibiotic treatment, it can be assumed that bovine DEFB-4 is an important endogenous parameter for the defense against bacterial infections of the udder.
Identifiants
pubmed: 37035820
doi: 10.3389/fvets.2023.1154386
pmc: PMC10079942
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1154386Informations de copyright
Copyright © 2023 Neumann, Siegert and Fischer.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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