Autophagy restricts Mycobacterium tuberculosis during acute infection in mice.
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
05 2023
05 2023
Historique:
received:
03
09
2021
accepted:
03
03
2023
medline:
8
5
2023
pubmed:
11
4
2023
entrez:
10
4
2023
Statut:
ppublish
Résumé
Whether or not autophagy has a role in defence against Mycobacterium tuberculosis infection remains unresolved. Previously, conditional knockdown of the core autophagy component ATG5 in myeloid cells was reported to confer extreme susceptibility to M. tuberculosis in mice, whereas depletion of other autophagy factors had no effect on infection. We show that doubling cre gene dosage to more robustly deplete ATG16L1 or ATG7 resulted in increased M. tuberculosis growth and host susceptibility in mice, although ATG5-depleted mice are more sensitive than ATG16L1- or ATG7-depleted mice. We imaged individual macrophages infected with M. tuberculosis and identified a shift from apoptosis to rapid necrosis in autophagy-depleted cells. This effect was dependent on phagosome permeabilization by M. tuberculosis. We monitored infected cells by electron microscopy, showing that autophagy protects the host macrophage by partially reducing mycobacterial access to the cytosol. We conclude that autophagy has an important role in defence against M. tuberculosis in mammals.
Identifiants
pubmed: 37037941
doi: 10.1038/s41564-023-01354-6
pii: 10.1038/s41564-023-01354-6
pmc: PMC11027733
mid: NIHMS1981926
doi:
Substances chimiques
Autophagy-Related Protein 5
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
819-832Subventions
Organisme : NIAID NIH HHS
ID : P01 AI063302
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI135990
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI162583
Pays : United States
Organisme : NIH HHS
ID : S10 OD010786
Pays : United States
Commentaires et corrections
Type : CommentIn
Type : ErratumIn
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature Limited.
Références
Cell Microbiol. 2008 Sep;10(9):1866-78
pubmed: 18503637
Nature. 2006 Jun 15;441(7095):885-9
pubmed: 16625204
Mol Immunol. 2018 May;97:16-19
pubmed: 29547747
Nat Microbiol. 2019 Dec;4(12):2128-2135
pubmed: 31611644
Front Immunol. 2020 Jan 09;10:3030
pubmed: 31998312
Nat Immunol. 2007 Jul;8(7):665-8
pubmed: 17579640
J Exp Med. 2008 Nov 24;205(12):2791-801
pubmed: 18955568
Cell. 2019 Jan 10;176(1-2):11-42
pubmed: 30633901
Nat Rev Immunol. 2015 Apr;15(4):255-63
pubmed: 25765201
J Cell Biol. 2017 Mar 6;216(3):583-594
pubmed: 28242744
J Cell Biol. 2005 May 9;169(3):425-34
pubmed: 15866887
Cell. 2015 Apr 23;161(3):501-512
pubmed: 25865481
Nat Microbiol. 2018 Dec;3(12):1472-1485
pubmed: 30478389
Mol Ther Methods Clin Dev. 2017 Sep 22;7:11-14
pubmed: 29057281
Cell Microbiol. 2012 Aug;14(8):1287-98
pubmed: 22524898
Cell Host Microbe. 2012 Apr 19;11(4):397-409
pubmed: 22520467
EMBO Rep. 2001 Apr;2(4):292-7
pubmed: 11306549
PLoS One. 2016 Mar 03;11(3):e0150606
pubmed: 26938654
ACS Infect Dis. 2017 Aug 11;3(8):564-574
pubmed: 28537707
Nat Cell Biol. 2021 May;23(5):450-456
pubmed: 33903736
Virulence. 2019 Dec;10(1):448-459
pubmed: 30322337
Cell Host Microbe. 2015 Jun 10;17(6):799-810
pubmed: 26048138
Genesis. 2003 Jul;36(3):162-7
pubmed: 12872248
Nature. 2016 May 5;533(7601):115-9
pubmed: 27096368
Mol Cell. 2020 Mar 5;77(5):951-969.e9
pubmed: 31995728
J Immunol Methods. 2014 Jun;408:89-100
pubmed: 24857755
Mucosal Immunol. 2011 May;4(3):279-87
pubmed: 21307848
J Cell Biol. 2022 Jul 4;221(7):
pubmed: 35699692
Nature. 2015 Dec 24;528(7583):482-3
pubmed: 26649822
Nature. 2014 Jul 3;511(7507):99-103
pubmed: 24990750
Mol Cell. 2018 Aug 16;71(4):637-648.e5
pubmed: 30118682
PLoS Pathog. 2018 Dec 31;14(12):e1007501
pubmed: 30596802
Front Immunol. 2017 Nov 24;8:1618
pubmed: 29225599
Nature. 2015 Dec 24;528(7583):565-9
pubmed: 26649827
Nature. 2012 Jan 15;482(7385):414-8
pubmed: 22246324
Nat Commun. 2020 Nov 4;11(1):5566
pubmed: 33149141
Cell. 2004 Dec 17;119(6):753-66
pubmed: 15607973
Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):E3168-76
pubmed: 23093667
PLoS Pathog. 2012 Feb;8(2):e1002507
pubmed: 22319448
Cell. 2012 Aug 17;150(4):803-15
pubmed: 22901810
Nucleic Acids Res. 2016 Jun 20;44(11):5356-64
pubmed: 27166376
Nat Cell Biol. 2006 Oct;8(10):1124-32
pubmed: 16998475
Autophagy. 2021 Jan;17(1):1-382
pubmed: 33634751
Infect Immun. 2008 Dec;76(12):5478-87
pubmed: 18852239
Nat Commun. 2013;4:2130
pubmed: 23945651
Mucosal Immunol. 2017 Nov;10(6):1553-1568
pubmed: 28401933
J Leukoc Biol. 2006 Jan;79(1):80-6
pubmed: 16275894
Nat Commun. 2020 May 8;11(1):2270
pubmed: 32385301
Nat Immunol. 2010 Aug;11(8):751-8
pubmed: 20622882
J Immunol. 2007 Mar 1;178(5):3143-52
pubmed: 17312162
Cell Host Microbe. 2016 Jan 13;19(1):102-13
pubmed: 26764600
J Immunol. 2010 Apr 1;184(7):3326-30
pubmed: 20200276
Proc Natl Acad Sci U S A. 2017 Feb 7;114(6):1371-1376
pubmed: 28119503
Cell Host Microbe. 2016 Jan 13;19(1):91-101
pubmed: 26764599
Elife. 2017 Jan 28;6:
pubmed: 28130921
Microbes Infect. 2015 Aug;17(8):564-74
pubmed: 25862076
Dev Cell. 2020 Jan 6;52(1):69-87.e8
pubmed: 31813797
Cell Host Microbe. 2012 Sep 13;12(3):289-300
pubmed: 22980326
Front Microbiol. 2017 Nov 23;8:2284
pubmed: 29218036
Nat Commun. 2012;3:1218
pubmed: 23169059
Cell Host Microbe. 2017 Oct 11;22(4):519-530.e3
pubmed: 29024644
Cell Host Microbe. 2015 Jun 10;17(6):811-819
pubmed: 26048136