Dynamic and Rapid Detection of Guanosine during Ischemia.
FSCV
guanosine
ischemia
neuroprotection
purines
voltammetry
Journal
ACS chemical neuroscience
ISSN: 1948-7193
Titre abrégé: ACS Chem Neurosci
Pays: United States
ID NLM: 101525337
Informations de publication
Date de publication:
03 05 2023
03 05 2023
Historique:
pmc-release:
03
05
2024
medline:
4
5
2023
pubmed:
12
4
2023
entrez:
11
4
2023
Statut:
ppublish
Résumé
Guanosine acts in both neuroprotective and neurosignaling pathways in the central nervous system; in this paper, we present the first fast voltammetric measurements of endogenous guanosine release during pre- and post-ischemic conditions. We discuss the metric of our measurements via analysis of event concentration, duration, and interevent time of rapid guanosine release. We observe changes across all three metrics from our normoxic to ischemic conditions. Pharmacological studies were performed to confirm that guanosine release is a calcium-dependent process and that the signaling observed is purinergic. Finally, we show the validity of our ischemic model via staining and fluorescent imaging. Overall, this paper sets the tone for rapid monitoring of guanosine and provides a platform to investigate the extent to which guanosine accumulates at the site of brain injury, i.e., ischemia.
Identifiants
pubmed: 37040534
doi: 10.1021/acschemneuro.3c00048
pmc: PMC10265669
mid: NIHMS1907010
doi:
Substances chimiques
Caffeine
3G6A5W338E
Calcium
SY7Q814VUP
Guanosine
12133JR80S
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1646-1658Subventions
Organisme : NINDS NIH HHS
ID : R01 NS121426
Pays : United States
Organisme : NINDS NIH HHS
ID : T32 NS007453
Pays : United States
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