Positive selection in cytochrome P450 genes is associated with gonad phenotype and mating strategy in social bees.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
11 04 2023
Historique:
received: 04 10 2022
accepted: 04 04 2023
medline: 13 4 2023
entrez: 11 4 2023
pubmed: 12 4 2023
Statut: epublish

Résumé

The honey bee, Apis mellifera differs from all other social bees in its gonad phenotype and mating strategy. Honey bee queens and drones have tremendously enlarged gonads, and virgin queens mate with several males. In contrast, in all the other bees, the male and female gonads are small, and the females mate with only one or very few males, thus, suggesting an evolutionary and developmental link between gonad phenotype and mating strategy. RNA-seq comparisons of A. mellifera larval gonads revealed 870 genes as differentially expressed in queens versus workers and drones. Based on Gene Ontology enrichment we selected 45 genes for comparing the expression levels of their orthologs in the larval gonads of the bumble bee Bombus terrestris and the stingless bee, Melipona quadrifasciata, which revealed 24 genes as differentially represented. An evolutionary analysis of their orthologs in 13 solitary and social bee genomes revealed four genes with evidence of positive selection. Two of these encode cytochrome P450 proteins, and their gene trees indicated a lineage-specific evolution in the genus Apis, indicating that cytochrome P450 genes may be involved in the evolutionary association of polyandry and the exaggerated gonad phenotype in social bees.

Identifiants

pubmed: 37041178
doi: 10.1038/s41598-023-32898-6
pii: 10.1038/s41598-023-32898-6
pmc: PMC10090045
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

5921

Informations de copyright

© 2023. The Author(s).

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Auteurs

Denyse Cavalcante Lago (DC)

Department of Genetics, Ribeirão Preto School of Medicine (FMRP), University of São Paulo (USP), Ribeirão Preto, SP, Brazil.

Luísa Czamanski Nora (LC)

Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto School of Medicine (FMRP), University of São Paulo (USP), Ribeirão Preto, SP, Brazil.

Martin Hasselmann (M)

Department of Livestock Population Genomics, Institute of Animal Science, University of Hohenheim, Stuttgart, Germany.

Klaus Hartfelder (K)

Department of Genetics, Ribeirão Preto School of Medicine (FMRP), University of São Paulo (USP), Ribeirão Preto, SP, Brazil. klaus@fmrp.usp.br.
Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto School of Medicine (FMRP), University of São Paulo (USP), Ribeirão Preto, SP, Brazil. klaus@fmrp.usp.br.

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