FXR1 regulates vascular smooth muscle cell cytoskeleton, VSMC contractility, and blood pressure by multiple mechanisms.
CP: Cell biology
WRC proteins
blood pressure
cytoskeletal
mRNA stability
vascular smooth muscle cell
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
25 04 2023
25 04 2023
Historique:
received:
12
10
2022
revised:
24
02
2023
accepted:
24
02
2023
medline:
4
10
2023
pubmed:
13
4
2023
entrez:
12
4
2023
Statut:
ppublish
Résumé
Appropriate cytoskeletal organization is essential for vascular smooth muscle cell (VSMC) conditions such as hypertension. This study identifies FXR1 as a key protein linking cytoskeletal dynamics with mRNA stability. RNA immunoprecipitation sequencing (RIP-seq) in human VSMCs identifies that FXR1 binds to mRNA associated with cytoskeletal dynamics, and FXR1 depletion decreases their mRNA stability. FXR1 binds and regulates actin polymerization. Mass spectrometry identifies that FXR1 interacts with cytoskeletal proteins, particularly Arp2, a protein crucial for VSMC contraction, and CYFIP1, a WASP family verprolin-homologous protein (WAVE) regulatory complex (WRC) protein that links mRNA processing with actin polymerization. Depletion of FXR1 decreases the cytoskeletal processes of adhesion, migration, contraction, and GTPase activation. Using telemetry, conditional FXR1
Identifiants
pubmed: 37043351
pii: S2211-1247(23)00392-3
doi: 10.1016/j.celrep.2023.112381
pmc: PMC10564969
mid: NIHMS1895598
pii:
doi:
Substances chimiques
Actins
0
Cytoskeletal Proteins
0
Muscle Proteins
0
FXR1 protein, human
0
RNA-Binding Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
112381Subventions
Organisme : NHLBI NIH HHS
ID : F31 HL160211
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL117724
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL141108
Pays : United States
Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors have no interests to disclose.
Références
Am J Physiol Cell Physiol. 2010 Dec;299(6):C1418-29
pubmed: 20881234
Neuron. 2013 Sep 18;79(6):1169-82
pubmed: 24050404
Arterioscler Thromb Vasc Biol. 2001 Mar;21(3):300-11
pubmed: 11231907
Cardiovasc Res. 2021 Feb 22;117(3):971-982
pubmed: 32384150
Circ Res. 2013 Oct 12;113(9):1065-75
pubmed: 23963726
Proc Natl Acad Sci U S A. 2013 Oct 15;110(42):16880-5
pubmed: 24085844
Nucleic Acids Res. 2022 Jan 7;50(D1):D497-D508
pubmed: 34718738
Biomol Concepts. 2011 Oct 1;2(5):343-52
pubmed: 25962042
J Biol Chem. 2005 Feb 18;280(7):5750-63
pubmed: 15548538
Nature. 2010 Nov 25;468(7323):533-8
pubmed: 21107423
Front Cell Neurosci. 2014 Mar 27;8:81
pubmed: 24733999
Brain Res Bull. 2001 Oct-Nov 1;56(3-4):375-82
pubmed: 11719275
Arterioscler Thromb Vasc Biol. 2018 Jun;38(6):1297-1308
pubmed: 29674474
Am J Physiol Cell Physiol. 2008 Sep;295(3):C576-87
pubmed: 18596210
RNA. 1999 Sep;5(9):1248-58
pubmed: 10496225
Curr Biol. 2021 May 24;31(10):R512-R517
pubmed: 34033782
Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8844-9
pubmed: 11438699
Sci Rep. 2017 Apr 19;7(1):962
pubmed: 28424484
Gen Pharmacol. 1996 Oct;27(7):1079-89
pubmed: 8981052
Proc Natl Acad Sci U S A. 2011 Aug 30;108(35):14449-54
pubmed: 21844371
Nat Rev Cardiol. 2010 Nov;7(11):637-47
pubmed: 20808285
Am J Pathol. 2016 May;186(5):1361-74
pubmed: 26952642
Nucleic Acids Res. 2007;35(1):299-306
pubmed: 17170008
J Mol Cell Cardiol. 2017 Apr;105:38-48
pubmed: 28257760
J Hum Hypertens. 2010 Jan;24(1):19-26
pubmed: 19474798
J Physiol. 2018 Aug;596(16):3617-3635
pubmed: 29746010
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Am J Physiol Cell Physiol. 2008 Sep;295(3):C768-78
pubmed: 18596213
Circ Res. 2003 Mar 7;92(4):411-8
pubmed: 12600888
Nat Rev Genet. 2012 Mar 06;13(4):246-59
pubmed: 22392217
Nat Med. 2008 Jan;14(1):64-8
pubmed: 18084302
J Proteome Res. 2011 Apr 1;10(4):1794-805
pubmed: 21254760
J Mol Cell Cardiol. 2015 Feb;79:157-68
pubmed: 25446180
Nat Methods. 2013 Aug;10(8):730-6
pubmed: 23921808
Trends Biochem Sci. 2003 Feb;28(2):69-74
pubmed: 12575993
Hum Mol Genet. 2004 Jul 1;13(13):1291-302
pubmed: 15128702
Hum Mol Genet. 1995 May;4(5):783-9
pubmed: 7633436
Am J Physiol Heart Circ Physiol. 2010 May;298(5):H1391-405
pubmed: 20154258
FASEB J. 2001 Apr;15(6):1062-4
pubmed: 11292668
Cell Rep. 2018 Jul 31;24(5):1176-1189
pubmed: 30067974
Arterioscler Thromb Vasc Biol. 2020 Apr;40(4):943-957
pubmed: 32075416
Nat Genet. 1993 Aug;4(4):335-40
pubmed: 8401578
Am J Physiol Cell Physiol. 2011 Apr;300(4):C896-906
pubmed: 21209363
Cell. 2001 Nov 16;107(4):477-87
pubmed: 11719188
J Biol Chem. 2012 Oct 5;287(41):33996-4008
pubmed: 22893699
Cell. 2011 Jul 22;146(2):247-61
pubmed: 21784246
FASEB J. 2019 Mar;33(3):3304-3316
pubmed: 30383449
Cardiovasc Res. 1995 Oct;30(4):544-56
pubmed: 8575003
Essays Biochem. 2019 Oct 31;63(5):483-495
pubmed: 31551324
Methods Mol Biol. 2006;341:103-9
pubmed: 16799192
J Mol Cell Cardiol. 2010 Oct;49(4):647-54
pubmed: 20451530
Hum Mol Genet. 2005 Mar 15;14(6):835-44
pubmed: 15703194
Nat Med. 2010 Feb;16(2):183-90
pubmed: 20098430
RNA. 2013 Nov;19(11):1483-96
pubmed: 24062571
Mol Cell Proteomics. 2012 Jun;11(6):M111.016444
pubmed: 22337587
Arterioscler Thromb Vasc Biol. 2019 Oct;39(10):2014-2027
pubmed: 31462091
Hum Mol Genet. 1995 Dec;4(12):2209-18
pubmed: 8634689
PLoS Biol. 2005 Nov;3(11):e383
pubmed: 16262445
J Cardiovasc Pharmacol Ther. 2008 Jun;13(2):130-40
pubmed: 18212360