OpcA and PorB are novel bactericidal antigens of the 4CMenB vaccine in mice and humans.
Journal
NPJ vaccines
ISSN: 2059-0105
Titre abrégé: NPJ Vaccines
Pays: England
ID NLM: 101699863
Informations de publication
Date de publication:
12 Apr 2023
12 Apr 2023
Historique:
received:
04
11
2022
accepted:
23
03
2023
medline:
13
4
2023
entrez:
12
4
2023
pubmed:
13
4
2023
Statut:
epublish
Résumé
The ability of Neisseria meningitidis Outer Membrane Vesicles (OMV) to induce protective responses in humans is well established and mainly attributed to Porin A (PorA). However, the contribution of additional protein antigens to protection remains to be elucidated. In this study we dissected the immunogenicity of antigens originating from the OMV component of the 4CMenB vaccine in mice and humans. We collected functional data on a panel of strains for which bactericidal responses to 4CMenB in infants was attributable to the OMV component and evaluated the role of 30 OMV-specific protein antigens in cross-coverage. By using tailor-made protein microarrays, the immunosignature of OMV antigens was determined. Three of these proteins, OpcA, NspA, and PorB, triggered mouse antibodies that were bactericidal against several N. meningitidis strains. Finally, by genetic deletion and/or serum depletion studies, we demonstrated the ability of OpcA and PorB to induce functional immune responses in infant sera after vaccination. In conclusion, while confirming the role of PorA in eliciting protective immunity, we identified two OMV antigens playing a key role in protection of infants vaccinated with the 4CMenB vaccine against different N. meningitidis serogroup B strains.
Identifiants
pubmed: 37045859
doi: 10.1038/s41541-023-00651-9
pii: 10.1038/s41541-023-00651-9
pmc: PMC10097807
doi:
Types de publication
Journal Article
Langues
eng
Pagination
54Informations de copyright
© 2023. The Author(s).
Références
Infect Immun. 1995 Dec;63(12):4642-52
pubmed: 7591118
Clin Vaccine Immunol. 2006 Apr;13(4):486-91
pubmed: 16603616
J Biotechnol. 2000 Sep 29;83(1-2):27-31
pubmed: 11000456
Sci Rep. 2019 May 2;9(1):6843
pubmed: 31048732
Vaccine. 2014 Mar 5;32(11):1280-6
pubmed: 24486354
J Infect Dis. 1993 May;167(5):1065-73
pubmed: 8486938
Infect Immun. 1994 Apr;62(4):1437-43
pubmed: 8132350
Scand J Immunol. 2013 Jun;77(6):452-9
pubmed: 23521186
Infect Immun. 1998 Oct;66(10):4755-61
pubmed: 9746575
Vaccine. 2017 Jul 24;35(33):4236-4244
pubmed: 28651840
Hum Vaccin. 2005 Mar-Apr;1(2):80-4
pubmed: 17038831
Epidemiol Infect. 1987 Dec;99(3):591-601
pubmed: 3123263
Vaccines (Basel). 2020 Sep 17;8(3):
pubmed: 32957610
NIPH Ann. 1991 Dec;14(2):195-207; discussion 208-10
pubmed: 1812432
Clin Infect Dis. 2010 Nov 15;51(10):1127-37
pubmed: 20954968
FASEB J. 2020 Aug;34(8):10329-10341
pubmed: 32725956
Expert Rev Vaccines. 2022 May;21(5):645-658
pubmed: 35257644
Vaccine. 2001 Jan 8;19(11-12):1327-46
pubmed: 11163654
Expert Rev Vaccines. 2013 Sep;12(9):981-4
pubmed: 24053391
Mol Med Rep. 2014 Sep;10(3):1619-25
pubmed: 24926810
Vaccine. 2010 Jul 12;28(31):5023-30
pubmed: 20493284
J Exp Med. 2003 Mar 17;197(6):789-99
pubmed: 12642606
Vaccine. 2005 Mar 18;23(17-18):2222-7
pubmed: 15755600
FEBS Lett. 2020 Aug;594(16):2670-2694
pubmed: 32058583
Clin Diagn Lab Immunol. 2005 Aug;12(8):970-6
pubmed: 16085915
Vaccine. 2010 Oct 8;28(43):6970-6
pubmed: 20732470
Lancet. 2017 Sep 30;390(10102):1603-1610
pubmed: 28705462
Vaccine. 2014 Mar 5;32(11):1273-9
pubmed: 24462403
Vaccine. 2001 Jan 8;19(11-12):1526-33
pubmed: 11163678
Expert Rev Vaccines. 2011 Mar;10(3):323-34
pubmed: 21434800
Sci Rep. 2018 Feb 27;8(1):3700
pubmed: 29487324
Mol Cell Proteomics. 2008 Mar;7(3):473-85
pubmed: 17982123
Vaccine. 2005 Mar 18;23(17-18):2191-6
pubmed: 15755593
Vaccine. 2011 Feb 24;29(10):1968-73
pubmed: 21241734
J Immunol. 2006 Jul 1;177(1):501-10
pubmed: 16785547
Proteomics. 2006 Jun;6(11):3400-13
pubmed: 16645985
Lancet. 1991 Nov 2;338(8775):1093-6
pubmed: 1682541
PLoS One. 2009 Jun 16;4(6):e5940
pubmed: 19529772
Expert Rev Vaccines. 2005 Jun;4(3):373-9
pubmed: 16026250
FEMS Immunol Med Microbiol. 2000 Feb;27(2):103-9
pubmed: 10640604
Infect Immun. 2007 Nov;75(11):5434-42
pubmed: 17664268
J Biol Chem. 1998 Sep 25;273(39):25329-38
pubmed: 9737999
Postgrad Med. 2016 Aug;128(6):548-56
pubmed: 27467048
Infect Immun. 1999 Nov;67(11):5664-75
pubmed: 10531214
Methods Mol Biol. 2009;498:91-103
pubmed: 18988020
J Biol Chem. 2003 Jul 4;278(27):24825-30
pubmed: 12716881
Vaccine. 2007 Jan 5;25(3):450-7
pubmed: 17052819
Vaccine. 2009 Mar 18;27(13):1912-22
pubmed: 19368771
Hum Vaccin Immunother. 2014;10(7):2005-14
pubmed: 25424810
Sex Transm Dis. 2021 Jul 1;48(7):453-457
pubmed: 33201019
Proteomics Clin Appl. 2007 Sep;1(9):1198-210
pubmed: 21136768
Infect Immun. 2002 Aug;70(8):4028-34
pubmed: 12117908
NPJ Vaccines. 2021 Oct 29;6(1):130
pubmed: 34716336
Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3770-5
pubmed: 20133713
Mol Microbiol. 1994 Jul;13(2):207-17
pubmed: 7984102
Infect Immun. 2015 Nov 23;84(2):452-8
pubmed: 26597984
Infect Immun. 2005 Apr;73(4):2424-32
pubmed: 15784588
PLoS Pathog. 2011 May;7(5):e1002027
pubmed: 21589640
mSphere. 2021 Feb 3;6(1):
pubmed: 33536323
Expert Opin Biol Ther. 2011 Jul;11(7):969-85
pubmed: 21615224
Lancet. 1983 Aug 13;2(8346):355-7
pubmed: 6135869
JAMA. 1999 Apr 28;281(16):1520-7
pubmed: 10227322
Infect Immun. 1998 Jul;66(7):3223-31
pubmed: 9632589
Gene. 2012 Jan 15;492(1):325-8
pubmed: 22037605
Lancet Infect Dis. 2010 Dec;10(12):853-61
pubmed: 21075057
J Extracell Vesicles. 2014 Aug 11;3:
pubmed: 25147647
Expert Rev Proteomics. 2014 Oct;11(5):573-91
pubmed: 25017717
Microb Cell. 2015 Mar 02;2(3):88-90
pubmed: 28357281
Clin Vaccine Immunol. 2007 Jul;14(7):830-8
pubmed: 17494638
Infect Immun. 1994 Oct;62(10):4419-24
pubmed: 7927704
Infect Immun. 1993 Nov;61(11):4734-42
pubmed: 8406872
Wellcome Open Res. 2018 Nov 29;3:151
pubmed: 30687793
Vaccine. 2022 Jun 21;40(28):3835-3842
pubmed: 35610106
Front Immunol. 2019 Apr 16;10:751
pubmed: 31040844
Vaccine. 2011 Feb 4;29(7):1413-20
pubmed: 21199704
Pediatr Infect Dis J. 2010 Nov;29(11):e71-9
pubmed: 20844462
Infect Immun. 1999 Mar;67(3):1267-76
pubmed: 10024570
NIPH Ann. 1991 Dec;14(2):67-79; discussion 79-80
pubmed: 1812438
J Immunol. 2011 Mar 15;186(6):3606-14
pubmed: 21325619
J Exp Med. 1997 Apr 7;185(7):1173-83
pubmed: 9104804
Expert Rev Vaccines. 2019 Jan;18(1):15-30
pubmed: 30526162
Infect Immun. 2001 Jun;69(6):3762-71
pubmed: 11349041
PLoS Pathog. 2020 Dec 8;16(12):e1008602
pubmed: 33290434
Hum Vaccin Immunother. 2013 Jun;9(6):1241-53
pubmed: 23857274
Lancet Infect Dis. 2022 Jul;22(7):1011-1020
pubmed: 35427492
Infect Immun. 1983 Apr;40(1):398-406
pubmed: 6131872
Proc Natl Acad Sci U S A. 2006 Jul 18;103(29):10834-9
pubmed: 16825336
J Extracell Vesicles. 2013 May 06;2:
pubmed: 24009891
Methods Mol Biol. 2017;1611:59-73
pubmed: 28451972