Transcriptional Regulation of hmsB, A Temperature-Dependent Small RNA, by RovM in Yersinia pestis Biovar Microtus.
Journal
Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448
Informations de publication
Date de publication:
13 Apr 2023
13 Apr 2023
Historique:
received:
09
09
2022
accepted:
04
11
2022
medline:
14
4
2023
entrez:
12
4
2023
pubmed:
13
4
2023
Statut:
epublish
Résumé
HmsB, a temperature-dependent sRNA, promotes biofilm formation by Yersinia pestis, but whether its own expression is regulated by other regulators is still poorly understood. RovM is a global regulator that activates biofilm formation but represses the virulence of Y. pestis. In this work, the results of primer extension, quantitative real-time PCR (qRT-PCR), and LacZ fusion demonstrated that RovM was able to activate hmsB expression. However, the results of electrophoretic mobility shift assay (EMSA) showed that His-RovM did not bind to the upstream DNA region of hmsB. Thus, RovM may exert its regulatory action on hmsB expression in an indirect manner. The data presented here enriched the content of the regulatory circuits that control gene expression in Y. pestis.
Identifiants
pubmed: 37046126
doi: 10.1007/s00284-023-03293-1
pii: 10.1007/s00284-023-03293-1
doi:
Substances chimiques
RNA
63231-63-0
Bacterial Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
182Subventions
Organisme : Zhejiang Province Public Welfare Technology Application Research Project
ID : 2020-ZJ-788
Organisme : National Natural Science Foundation of China
ID : 81801984
Organisme : China Postdoctoral Science Foundation
ID : 2019M664008
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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