Transmembrane Mucin Response in Conjunctival Epithelial Cells Exposed to Wall Shear Stresses.
F-actin
MUC1
MUC16
mechanobiology
membrane-tethered mucins
primary human cells
steady airflow
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
01 Apr 2023
01 Apr 2023
Historique:
received:
26
01
2023
revised:
08
03
2023
accepted:
20
03
2023
medline:
14
4
2023
entrez:
13
4
2023
pubmed:
14
4
2023
Statut:
epublish
Résumé
Human conjunctival epithelium cells (HCEC) line the inner surface of the eyelid and cover the sclera and are continuously subjected to wall shear stresses (WSS). The effects of external forces on the conjunctival epithelium are not fully known. The conjunctival epithelium contains stratified squamous cells that synthesize the membrane-spanning mucins MUC1 and MUC16, which play important roles in protecting the ocular surface. Alterations in both gel-forming and membrane-tethered mucins occur in drying ocular surface diseases. The aim of this study was to explore the mechanobiological characteristics of transmembrane mucin secretion and cellular alterations of primary HCEC exposed to airflow-induced WSS perturbations. We exposed the HCEC to a steady WSS of 0.5 dyne/cm
Identifiants
pubmed: 37047561
pii: ijms24076589
doi: 10.3390/ijms24076589
pmc: PMC10095083
pii:
doi:
Substances chimiques
Mucins
0
Mucin-1
0
CA-125 Antigen
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Israel Science Foundation
ID : 1481/18
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