In Vitro Effect of Enzymes and Human Milk Oligosaccharides on FODMAP Digestion and Fecal Microbiota Composition.

FODMAP digestive enzymes human milk oligosaccharide inflammatory bowel syndrome invertase α-galactosidase β-galactosidase

Journal

Nutrients
ISSN: 2072-6643
Titre abrégé: Nutrients
Pays: Switzerland
ID NLM: 101521595

Informations de publication

Date de publication:
28 Mar 2023
Historique:
received: 23 12 2022
revised: 15 01 2023
accepted: 07 02 2023
medline: 14 4 2023
entrez: 13 4 2023
pubmed: 14 4 2023
Statut: epublish

Résumé

Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) cause intestinal discomfort in patients with irritable bowel syndrome (IBS). An enzyme mix (2500 SU invertase, 2400 GalU α-galactosidase, 10,000 ALU β-galactosidase) optimized for FODMAP digestion, and/or human milk oligosaccharides (HMO) (2'-FL, DFL, and LNnT), were evaluated for effects on microbial community activity and composition in short-term colonic incubations using the fecal microbiota of four patients with IBS-D symptoms under the following test conditions: (i) FODMAP, (ii) pre-digested (with enzyme mix) FODMAP, (iii) FODMAP + HMO, and (iv) pre-digested FODMAP + HMO. Pre-digested FODMAP reduced short-chain fatty acid (SCFA) production versus FODMAP; HMO restored this. A 10-day experiment with the simulator of the human intestinal microbial ecosystem (SHIME

Identifiants

pubmed: 37049481
pii: nu15071637
doi: 10.3390/nu15071637
pmc: PMC10097142
pii:
doi:

Substances chimiques

Oligosaccharides 0
Fatty Acids, Volatile 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : DSM
ID : Company funds

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Auteurs

Van T Pham (VT)

DSM Nutritional Products, Wurmisweg 576, 4303 Kaiseraugst, Switzerland.

Robert E Steinert (RE)

DSM Nutritional Products, Wurmisweg 576, 4303 Kaiseraugst, Switzerland.

Cindy Duysburgh (C)

ProDigest, Technologiepark 82, Zwijnaarde, 9052 Ghent, Belgium.

Jonas Ghyselinck (J)

ProDigest, Technologiepark 82, Zwijnaarde, 9052 Ghent, Belgium.

Massimo Marzorati (M)

ProDigest, Technologiepark 82, Zwijnaarde, 9052 Ghent, Belgium.
Center for Microbial Ecology and Technology, Faculty of Bioscience Engineering, University of Ghent, Coupure Links 653, 9000 Ghent, Belgium.

Peter J T Dekker (PJT)

DSM Food & Beverage, Center for Food Innovation, Fleminglaan 1, 2613 AX Delft, The Netherlands.

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Classifications MeSH