Metastatic Melanoma: A Preclinical Model Standardization and Development of a Chitosan-Coated Nanoemulsion Containing Temozolomide to Treat Brain Metastasis.
Brain metastasis
Chitosan
Melanoma
Nanoemulsion
Temozolomide
Journal
Cellular and molecular neurobiology
ISSN: 1573-6830
Titre abrégé: Cell Mol Neurobiol
Pays: United States
ID NLM: 8200709
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
received:
09
03
2022
accepted:
13
03
2023
medline:
12
7
2023
pubmed:
14
4
2023
entrez:
13
4
2023
Statut:
ppublish
Résumé
Melanoma is the most aggressive type of skin cancer. Brain metastasis is the worst scenario in metastatic melanoma and the treatment options for these patients are limited. Temozolomide (TMZ) is a chemotherapy agent used to treat primary central nervous system tumors. Our objective was to develop chitosan-coated nanoemulsion containing temozolomide (CNE-TMZ) for nasal route administration to melanoma brain metastasis treatment. A preclinical model of metastatic brain melanoma was standardized, and the efficiency of the developed formulation was further determined in vitro and in vivo. The nanoemulsion was done by spontaneous emulsification method and the formulation was characterized by size, pH, polydispersity index, and zeta potential. Culture assessments to determine cell viability were done in the A375 human melanoma cell line. To determine the safety of formulation, healthy C57/BL6 mice were treated with a nanoemulsion without TMZ. The model in vivo used B16-F10 cells implanted by stereotaxic surgery in C57/BL6 mice brains. The results demonstrate that the preclinical model used showed to be useful to analyze the efficiency of new candidate drugs to treat melanoma brain metastasis. The chitosan-coated nanoemulsions with TMZ showed the expected physicochemical characteristics and demonstrated safety and efficacy, reducing around 70% the tumor size compared to control mice, and presenting a tendency in mitotic index reduction, becoming an interesting approach to treat melanoma brain metastasis.
Identifiants
pubmed: 37055607
doi: 10.1007/s10571-023-01338-4
pii: 10.1007/s10571-023-01338-4
doi:
Substances chimiques
Temozolomide
YF1K15M17Y
Chitosan
9012-76-4
Dacarbazine
7GR28W0FJI
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2939-2951Subventions
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : 001
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 422298/2016-6
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 310846/2014-5
Organisme : Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul
ID : 16/2551-0000265-7
Organisme : Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul
ID : 16/2551-0000473-0
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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