Storage stability and HILIC-UHPLC-FLR analysis of immunoglobulin G N-glycome from saliva.
Biomarker
Immunoglobulin G
N-Glycosylation
Saliva
Ultra-high-performance liquid chromatography
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
03
02
2023
accepted:
31
03
2023
revised:
17
03
2023
medline:
10
11
2023
pubmed:
15
4
2023
entrez:
14
4
2023
Statut:
ppublish
Résumé
Immunoglobulin G (IgG) is the most abundant antibody in the blood and plays a critical role in host immune defense against infectious agents. Glycosylation is known to modulate the effector functions of IgG and is involved in disease development and progression. It is no surprise that the N-glycome of IgG from plasma has already been proposed as a biomarker for various physiological and pathological conditions. However, because saliva is easy to collect, it could be useful for exploring the functional role of salivary IgG N-glycosylation and its potential as a diagnostic biomarker. Therefore, in this study, we described a method for N-glycome analysis of IgG from saliva samples. Salivary IgG N-glycans were analyzed by ultra-high-performance liquid chromatography based on hydrophilic interactions with fluorescence detection (HILIC-UHPLC-FLR). In addition, we compared IgG N-glycan profiles from saliva with those from plasma, assessed the stability of salivary IgG N-glycan profiles under different storage conditions, and evaluated the effects of using a saliva preservation medium. This study provides an ultrasensitive UHPLC method for the analysis of total IgG N-glycosylation from saliva, gives insight into storage stability of salivary IgG, and highlights its (dis)advantages for further biomarker-related research.
Identifiants
pubmed: 37058166
doi: 10.1007/s00216-023-04682-y
pii: 10.1007/s00216-023-04682-y
pmc: PMC10101819
doi:
Substances chimiques
Immunoglobulin G
0
Polysaccharides
0
Biomarkers
0
Immunoglobulin A, Secretory
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6985-6993Subventions
Organisme : Hrvatska Zaklada za Znanost
ID : UIP-2019-04-5692
Informations de copyright
© 2023. Springer-Verlag GmbH Germany, part of Springer Nature.
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